_ASSAY_METADATA.TXT Version 1
_p_RNAinVAL/_I_01_LabTrials/_S_09_jun2017/_A_03-qPCR/
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Assay: _A_03-qPCR Short Name: 03-qPCR Assay Class: WET Assay Type: qPCR Title: qPCR for target genes Description: Quantitative PCR (qPCR) for selected target genes using the established methodology at NIB. pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Phenodata: ../../phenodata_20210113.txt Featuredata: Data: Lab manager: Kristina Gruden Assay chemistry: mixed Assay optimisation protocol (file path, link or citation): Pippeting: liquid handling station Reaction volume: 5 ul qPCR platform: HT7900 Fast, ABI (SDS) qPCR protocol (file path, link or citation): https://dx.doi.org/10.3389%2Ffpls.2020.01250 One-step RT-PCR (RNA as input): No Well format: 384 MasterMix: TaqMan Universal PCR Master mix, ABI (4318157); Power SYBR® Green PCR Master Mix, ABI (4367659) No. sample technical replicates: 2 No. sample dilutions: 2 PCR controls used: NTC only No. standard curve dilutions (if using): 5 Operator: Katja Stare Cq value determination software (if different from PCR platform): Quantification software: quantGenius qPCR quantification method: relative standard curve Notes:
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Created: 10th Nov 2021 at 09:36
Last updated: 10th Nov 2021 at 09:36
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Version 1 (earliest) Created 10th Nov 2021 at 09:36 by Andrej Blejec
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Institutions: National Institute of Biology, NIB, <script>alert('xss-institution-title')</script> & Test Institution
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Test upload using seekr before upload to FAIRDOMHub
Programme: pISA-tree
Public web page: Not specified
Short Name: 01_LabTrials Title: Laboratory trials Description: Selection of targets and their validation in trials performed in the laboratories and greenhouse at NIB Phenodata: ./phenodata_20210113.txt pISA Investigation creation date: 2021-01-13 pISA Investigation creator: Marko Petek Principal investigator: Marko Petek License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Andrej Blejec
Studies: Investigation files, _S_01_TargetSelect, _S_02_dsRNAorder, _S_03_dsRNAprod, _S_04_Stages, _S_05_jun2016, _S_06_oct2016, _S_07_dec2016, _S_08_jan2017, _S_09_jun2017, _S_10_apr2018, _S_11_may2018
Assays: _A_00_Ecoli-dry, _A_00_dsRNA_stabil-wet, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01_AgroRNA-wet, _A_01_LitData-dry, _A_01_pIsol-wet, _A_02-RNAisol, _A_02-RNAisol, _A_02-RNAisol, _A_02_UlrichTop100-BLAST, _A_02_plasmid-SangerSeq, _A_02_qPCR_ampl_test-wet, _A_03-qPCR, _A_03-qPCR, _A_03-qPCR, _A_03_RNaseItreat-wet, _A_03_patentDB-BLAST, _A_03_stages-RNAisol, _A_04_ortho-BLAST, _A_04_prod-qPCR, _A_04_stages-qPCR, _A_05_CPB_gene-annot, _A_06_splitter_BLAST-dry, _A_07_MergeEvi-dry, _A_08_SelTargetsA-dry, _I_01_LabTrials-files, _S_01_TargetSelect-files, _S_02_dsRNAorder-files, _S_03_dsRNAprod-files, _S_04_Stages-files, _S_05_jun2016-files, _S_06_oct2016-files, _S_07_dec2016-files, _S_08_jan2017-files, _S_09_jun2017-files, _S_10_apr2018-files, _S_11_may2018-files
Short Name: 09_jun2017 Title: CPB feeding experiment jun2017 Description: Colorado potato beetle dsRNA feeding trial performed in June 2017 Raw Data: pISA Study creation date: 2021-05-10 pISA Study creator: Marko Petek Principal investigator: Marko Petek License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Andrej Blejec
Investigation: _I_01_LabTrials
Assays: _A_00_dsRNA_stabil-wet, _A_01-phenotyping, _A_02-RNAisol, _A_03-qPCR, _S_09_jun2017-files
Short Name: 03-qPCR Assay Class: WET Assay Type: qPCR Title: qPCR for target genes Description: Quantitative PCR (qPCR) for selected target genes using the established methodology at NIB. pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Phenodata: ../../phenodata_20210113.txt Featuredata: Data: Lab manager: Kristina Gruden Assay chemistry: mixed Assay optimisation protocol (file path, link or citation): Pippeting: liquid handling station Reaction volume: 5 ul qPCR platform: ...
Submitter: Andrej Blejec
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_01_LabTrials
Study: _S_09_jun2017
