_A_03-qPCR

Short Name: 03-qPCR Assay Class: WET Assay Type: qPCR Title: qPCR for target genes Description: Quantitative PCR (qPCR) for selected target genes using the established methodology at NIB. pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Phenodata: ../../phenodata_20210113.txt Featuredata: Data: ../../_S_07_dec2016/_A_03-qPCR/output Lab manager: Kristina Gruden Assay chemistry: mixed Assay optimisation protocol (file path, link or citation): Pippeting: liquid handling station Reaction volume: 5 ul qPCR platform: HT7900 Fast, ABI (SDS) qPCR protocol (file path, link or citation): https://dx.doi.org/10.3389%2Ffpls.2020.01250 One-step RT-PCR (RNA as input): No Well format: 384 MasterMix: TaqMan Universal PCR Master mix, ABI (4318157); Power SYBR® Green PCR Master Mix, ABI (4367659) No. sample technical replicates: 2 No. sample dilutions: 2 PCR controls used: NTC only No. standard curve dilutions (if using): 5 Operator: Katja Stare Cq value determination software (if different from PCR platform): Quantification software: quantGenius qPCR quantification method: relative standard curve Notes: