_A_04_prod-qPCR
Single PageShort Name: 04_prod-qPCR Assay Class: WET Assay Type: qPCR Title: qPCR for quantification of dsRNA production in E coli Description: qPCR for quantification of dsRNA production in E coli pISA Assay creation date: 2021-01-14 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data:
SEEK ID: https://testing.sysmo-db.org/assays/781
Experimental assay
Projects: _p_RNAinVAL
Investigation: _I_01_LabTrials
Study: _S_03_dsRNAprod
Assay position:
Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: No organisms
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Created: 10th Nov 2021 at 09:34
Last updated: 10th Nov 2021 at 15:20
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Projects: testUpload, Test project 2019-10-30 11:13:00, Test project 2019-10-30 11:09:39, Test project 2019-11-02 19:40:30, Test project for pISA (104), _p_stRT, _p_Demo, Bla, Test project abcd, Test project 2021-07-18 22:00:39, Test project 2021-07-18 22:45:54, Test project 2021-07-18 23:08:33, Test project 2021-07-18 23:23:02, Test project 2021-07-21 00:08:04, Test project 2021-07-21 00:17:06, Test project 2021-07-21 00:18:25, Test project 2021-08-20 22:56:43, Testing of upload, _p_RNAinVAL
Institutions: National Institute of Biology, NIB, <script>alert('xss-institution-title')</script> & Test Institution
https://orcid.org/0000-0001-7484-6031
Local data management; testing uploading/downloading using API
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Web page: Not specified
Test upload using seekr before upload to FAIRDOMHub
Programme: pISA-tree
Public web page: Not specified
Short Name: 01_LabTrials Title: Laboratory trials Description: Selection of targets and their validation in trials performed in the laboratories and greenhouse at NIB Phenodata: ./phenodata_20210113.txt pISA Investigation creation date: 2021-01-13 pISA Investigation creator: Marko Petek Principal investigator: Marko Petek License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Andrej Blejec
Studies: Investigation files, _S_01_TargetSelect, _S_02_dsRNAorder, _S_03_dsRNAprod, _S_04_Stages, _S_05_jun2016, _S_06_oct2016, _S_07_dec2016, _S_08_jan2017, _S_09_jun2017, _S_10_apr2018, _S_11_may2018
Assays: _A_00_Ecoli-dry, _A_00_dsRNA_stabil-wet, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01_AgroRNA-wet, _A_01_LitData-dry, _A_01_pIsol-wet, _A_02-RNAisol, _A_02-RNAisol, _A_02-RNAisol, _A_02_UlrichTop100-BLAST, _A_02_plasmid-SangerSeq, _A_02_qPCR_ampl_test-wet, _A_03-qPCR, _A_03-qPCR, _A_03-qPCR, _A_03_RNaseItreat-wet, _A_03_patentDB-BLAST, _A_03_stages-RNAisol, _A_04_ortho-BLAST, _A_04_prod-qPCR, _A_04_stages-qPCR, _A_05_CPB_gene-annot, _A_06_splitter_BLAST-dry, _A_07_MergeEvi-dry, _A_08_SelTargetsA-dry, _I_01_LabTrials-files, _S_01_TargetSelect-files, _S_02_dsRNAorder-files, _S_03_dsRNAprod-files, _S_04_Stages-files, _S_05_jun2016-files, _S_06_oct2016-files, _S_07_dec2016-files, _S_08_jan2017-files, _S_09_jun2017-files, _S_10_apr2018-files, _S_11_may2018-files
Short Name: 03_dsRNAprod Title: Production of selected desigend dsRNAs in-vivo Description: We established the production of two selected designed dsRNAs in-vivo in bacterial cultures of E. coli TH115 (DE3), a strain that does not produce RNases and is thus suitable for production of long dsRNA Raw Data: pISA Study creation date: 2021-01-14 pISA Study creator: Marko Petek Principal investigator: Marko Petek License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Andrej Blejec
Investigation: _I_01_LabTrials
Assays: _A_00_Ecoli-dry, _A_01_pIsol-wet, _A_02_plasmid-SangerSeq, _A_03_RNaseItreat-wet, _A_04_prod-qPCR, _S_03_dsRNAprod-files
_p_RNAinVAL/_I_01_LabTrials/_S_03_dsRNAprod/_A_04_prod-qPCR/
_p_RNAinVAL/_I_01_LabTrials/_S_03_dsRNAprod/_A_04_prod-qPCR/
_p_RNAinVAL/_I_01_LabTrials/_S_03_dsRNAprod/_A_04_prod-qPCR/
_p_RNAinVAL/_I_01_LabTrials/_S_03_dsRNAprod/_A_04_prod-qPCR/
_p_RNAinVAL/_I_01_LabTrials/_S_03_dsRNAprod/_A_04_prod-qPCR/
_p_RNAinVAL/_I_01_LabTrials/_S_03_dsRNAprod/_A_04_prod-qPCR/
_p_RNAinVAL/_I_01_LabTrials/_S_03_dsRNAprod/_A_04_prod-qPCR/
_p_RNAinVAL/_I_01_LabTrials/_S_03_dsRNAprod/_A_04_prod-qPCR/
Creator: Andrej Blejec
Submitter: Andrej Blejec
