GLC incubation

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SEEK ID: https://testing.sysmo-db.org/assays/57

Class: Modelling analysis

Projects: SysMO DB

Investigation: Methods for interrelating 'omics data

Study: Metabolomics data capture

Assay position:

Biological problem addressed: Metabolism

Organisms: Bacillus subtilis, Clostridium acetobutylicum, Bacillus subtilis : 168b (del ase / dasfadsf)

Model:

Kinetic model for incubation

Data:

5mM GLC runout data.
Quyen data

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Creators and Submitter

Creator

Quyen Yamanaka

Submitter

Quyen Yamanaka

Activity

Views: 1169

Created: 16th Nov 2015 at 16:49

Last updated: 26th Sep 2017 at 10:54

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People (1)
Programmes (1)
Projects (1)
Investigations (1)
Studies (1)
Strains (1)
Data files (2)
Models (1)
SOPs (3)
Publications (1)

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Quyen Yamanaka

Projects: SysMO DB, SysMOLAB, SilicoTryp, maggy's project

Institutions: HITS gGmbH, University of Heidelberg, Somewhere Else

Expertise: Bioinformatics

Tools: Ruby on Rails

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Dummy programme

No description specified

Projects: SysMO DB

Web page: Not specified

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SysMO DB

The main objectives of SysMO-DB are to: facilitate the web-based exchange of data between research groups within- and inter- consortia, and to provide an integrated platform for the dissemination of the results of the SysMO projects to the scientific community. We aim to devise a progressive and scalable solution to the data management needs of the SysMO initiative, that:

maximises the ‘shelf life’ and utility of data generated by SysMO;
provides an integrated platform for the dissemination ...

Programme: Dummy programme

Public web page: http://www.sysmo-db.org/

Organisms: Lactic Acid Bacteria, Agapornis

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Methods for interrelating 'omics data

SysMO DB

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Olga Krebs

This Investigation was performed by SySMO DB team together with PALS in order to crete methods and best practices for capturing experimental data.

Submitter: Olga Krebs

Studies: Metabolomics data capture

Assays: Excel template for metabolomics data, GLC incubation

Snapshots: No snapshots

Created: 10th Jun 2009 at 17:09, Last updated: 10th Nov 2011 at 17:14

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Metabolomics data capture

SysMO DB

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Olga Krebs

No description specified

Submitter: Olga Krebs

Investigation: Methods for interrelating 'omics data

Assays: Excel template for metabolomics data, GLC incubation

Snapshots: No snapshots

Created: 10th Jun 2009 at 17:10, Last updated: 10th Nov 2011 at 17:14

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168b

SysMO DB

Submitter: queeny Nguyen

Provider Name: Not specified

Provider's strain ID: Not specified

Organism: Bacillus subtilis

Genotypes: del ase

Phenotypes: dasfadsf

Comment: ss

Created: 5th Jun 2012 at 11:30

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5mM GLC runout data.

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Stuart Owen

No description specified

Creator: Stuart Owen

Submitter: Stuart Owen

Relationship type: Not specified

Investigations: Glucose metabolism in Plasmodium falciparum tro..., Methods for interrelating 'omics data

Studies: Metabolomics data capture, Model validation

Assays: GLC incubation, GLC incubation

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Created: 10th Jul 2015 at 14:24, Last updated: 10th Jul 2015 at 14:29

Quyen data

SysMO DB

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Quyen Yamanaka

No description specified

Creator: Quyen Yamanaka

Submitter: Quyen Yamanaka

DOI: 10.5072/seek.datafile.80.2

Relationship type: Construction data

Investigations: Growth control of the eukaryote cell: a systems..., Methods for interrelating 'omics data

Studies: Metabolomics data capture and 1 hidden item

Assays: GLC incubation, test assay

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Created: 11th Jun 2012 at 09:55, Last updated: 26th Jun 2015 at 10:27

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Kinetic model for incubation

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Stuart Owen

Glycolytic model for Plasmodium falciparum; closed system

Creator: Stuart Owen

Submitter: Stuart Owen

Model type: Not specified

Model format: Not specified

Environment: Not specified

DOI: 10.5072/seek.model.98.1

Organism: Not specified

Investigations: Glucose metabolism in Plasmodium falciparum tro..., Methods for interrelating 'omics data

Studies: Metabolomics data capture, Model validation

Assays: GLC incubation, GLC incubation

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Created: 10th Jul 2015 at 14:23, Last updated: 10th Jul 2015 at 14:29

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Validation experiments

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Stuart Owen

Finn Bacall

SOP for the determination of external metabolites (Glc, Pyr, Gly, Lac) in intact trophozoite incubations, and for the determination of intracellular metabolite concentrations.

Creators: Stuart Owen, Finn Bacall

Submitter: Stuart Owen

Investigations: Glucose metabolism in Plasmodium falciparum tro..., Investigation with some private bits, Methods for interrelating 'omics data

Studies: Metabolomics data capture, Model validation, Public Study for OPSK-457

Assays: GLC incubation, GLC incubation, testing extraction title

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Created: 10th Jul 2015 at 14:22, Last updated: 2nd Mar 2017 at 14:18

Quyen's sop

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Quyen Yamanaka

edited to test subscription emails

Creators: None

Submitter: Quyen Yamanaka

Investigations: An example Investigation with Scale, Methods for interrelating 'omics data

Studies: Metabolomics data capture, a test study

Assays: Assay with a SOP, GLC incubation, Modelling Assay with a SOP

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Created: 11th Jun 2012 at 08:24, Last updated: 5th Apr 2013 at 09:02

^K blabla

SysMO DB

No description specified

Creators: None

Submitter: Deleted submitter

Investigations: Methods for interrelating 'omics data

Studies: Metabolomics data capture

Assays: GLC incubation

Created: 9th Mar 2012 at 14:27

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Phenomenon of hot-cold hemolysis: chelator-induced lysis of sphingomyelinase-treated erythrocytes

SysMO DB

Abstract (Expand)

Staphylococcus aureus produces a phospholipase C specific for sphingomyelin (beta-hemolysin). Erythrocytes with approximately 50% sphingomyelin in their membranes, e.g., from sheep, have been shown to … have up to 60% of this phospholipid hydrolyzed by this enzyme at 37 C in isotonic buffered saline without hemolysis. Cooling of sphingomyelinase C-treated erythrocytes to 4 C causes complete lysis of the cells, a phenomenon known as hot-cold hemolysis. The addition of ethylenediaminetetraacetate (EDTA) to sheep erythrocytes preincubated with sphingomyelinase C was found to induce rapid hemolysis at 37 C. The treated cells became susceptible to chelator-induced hemolysis and to hot-cold hemolysis simultaneously, and the degree of lysis of both mechanisms increased equally with prolonged preincubation with sphingomyelinase C. Erythrocytes of species not readily susceptible to hot-cold hemolysis were equally insusceptible to chelator-induced lysis. Chelators of the EDTA series were the most effective, whereas chelators more specific for Ca2+, Zn2+, Fe2+, Cu2+, and Mg2+ were without effect. The rate of chelator-induced lysis was dependent on the preincubation period with beta-hemolysin and on the concentration of chelator added. The optimal concentration of EDTA was found to equal the amount of exogenously added Mg2+, a cation necessary for sphingomyelinase C activity. Hypotonicity increased the rate of chelator-induced hemolysis, whereas increasing the osmotic pressure to twice isotonic completely inhibited chelator-induced lysis. The data suggest that exogenously added and/or membrane-bound divalent cations are important for the stability of sphingomyelin-depleted membranes. The phenomenon of hot-cold hemolysis may be a consequence of the temperature dependence of divalent ion stabilization.

Authors: C J Smyth, R Möllby, T Wadström

Date Published: 1st Nov 1975

Publication Type: Not specified

PubMed ID: 333

Citation:

Created: 21st Sep 2012 at 13:53, Last updated: 24th Mar 2022 at 10:39