example assay
No description specified

Submitter: Quyen Yamanaka

Biological problem addressed: Model Analysis Type

Investigation: Glycolitic Flux in Lactococcus lactis

Study: 1 hidden item

Aitor's data file

Some text file Aitor uploaded

Creator: Aitor Apaolaza

Submitter: Aitor Apaolaza

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test exploring
No description specified

Creators: None

Submitter: Lihua Angel

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Spreadsheet Indexing Test

To see if the google crawler will pick out the keys in the spreadsheet metadata.

1ygrVB04jL

Creator: Stuart Owen

Submitter: Stuart Owen

External Link
large file
No description specified

Creator: Stuart Owen

Submitter: Stuart Owen

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Teusink Model
No description specified

Creator: Jacky Snoep

Submitter: Guest

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my model
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Creator: Quyen Yamanaka

Submitter: Quyen Yamanaka

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new sop
No description specified

Creator: Lihua Angel

Submitter: queeny Nguyen

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COGEME_extraction_mod-chemostat_v1

Biomass samples (20ml) were taken via the sample port of the Applikon fermenters. The cells were pelleted by centrifugation for 5min at 5000rpm. The supernatant was removed and the RNA pellet resuspended in the residual medium to form a slurry. This was added in a dropwise manner directly into a 5ml Teflon flask (B. Braun Biotech, Germany) containing liquid nitrogen and a 7mm-diameter tungsten carbide ball.

Taken from ArrayExpress: http://www.ebi.ac.uk/microarray-as/ae/browse.html?keywords=E-MEXP-115 ...

Creator: Katy Wolstencroft

Submitter: Katy Wolstencroft

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Phenomenon of hot-cold hemolysis: chelator-induced lysis of sphingomyelinase-treated erythrocytes

Abstract (Expand)

Staphylococcus aureus produces a phospholipase C specific for sphingomyelin (beta-hemolysin). Erythrocytes with approximately 50% sphingomyelin in their membranes, e.g., from sheep, have been shown to … have up to 60% of this phospholipid hydrolyzed by this enzyme at 37 C in isotonic buffered saline without hemolysis. Cooling of sphingomyelinase C-treated erythrocytes to 4 C causes complete lysis of the cells, a phenomenon known as hot-cold hemolysis. The addition of ethylenediaminetetraacetate (EDTA) to sheep erythrocytes preincubated with sphingomyelinase C was found to induce rapid hemolysis at 37 C. The treated cells became susceptible to chelator-induced hemolysis and to hot-cold hemolysis simultaneously, and the degree of lysis of both mechanisms increased equally with prolonged preincubation with sphingomyelinase C. Erythrocytes of species not readily susceptible to hot-cold hemolysis were equally insusceptible to chelator-induced lysis. Chelators of the EDTA series were the most effective, whereas chelators more specific for Ca2+, Zn2+, Fe2+, Cu2+, and Mg2+ were without effect. The rate of chelator-induced lysis was dependent on the preincubation period with beta-hemolysin and on the concentration of chelator added. The optimal concentration of EDTA was found to equal the amount of exogenously added Mg2+, a cation necessary for sphingomyelinase C activity. Hypotonicity increased the rate of chelator-induced hemolysis, whereas increasing the osmotic pressure to twice isotonic completely inhibited chelator-induced lysis. The data suggest that exogenously added and/or membrane-bound divalent cations are important for the stability of sphingomyelin-depleted membranes. The phenomenon of hot-cold hemolysis may be a consequence of the temperature dependence of divalent ion stabilization.

Authors: C J Smyth, R Möllby, T Wadström

Date Published: 1st Nov 1975

Publication Type: Not specified

a test
No description specified

Creator: Stuart Owen

Submitter: Stuart Owen

New features in SEEK

David's presentation from PALs meeting in Heidelberg

Creators: Olga Krebs, David Jones, Quyen Yamanaka, Miss X

Submitter: Olga Krebs

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