COGEME_extraction_mod-chemostat_v1 Version 1
Biomass samples (20ml) were taken via the sample port of the Applikon fermenters. The cells were pelleted by centrifugation for 5min at 5000rpm. The supernatant was removed and the RNA pellet resuspended in the residual medium to form a slurry. This was added in a dropwise manner directly into a 5ml Teflon flask (B. Braun Biotech, Germany) containing liquid nitrogen and a 7mm-diameter tungsten carbide ball.
Taken from ArrayExpress: http://www.ebi.ac.uk/microarray-as/ae/browse.html?keywords=E-MEXP-115
SEEK ID: https://testing.sysmo-db.org/sops/15?version=1
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COGEME_BII_1SOP.doc
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Created: 3rd Dec 2009 at 11:24
Last updated: 5th Apr 2013 at 08:58
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Version 1 (earliest) Created 3rd Dec 2009 at 11:24 by Katy Wolstencroft
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Projects: SysMO DB, Virtual Liver Network Developers
Institutions: School of Computer Science, University of Manchester, HITS gGmbH
I am a Research Fellow at the University of Manchester, working in Bioinformatics and Computer Science. I am also a guest researcher at the Vrije Universiteit.My research interests include scientific workflows, semantic discovery, and applying ontology technologies to biological data
The main objectives of SysMO-DB are to: facilitate the web-based exchange of data between research groups within- and inter- consortia, and to provide an integrated platform for the dissemination of the results of the SysMO projects to the scientific community. We aim to devise a progressive and scalable solution to the data management needs of the SysMO initiative, that:
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Public web page: http://www.sysmo-db.org/
Cell growth underlies many key cellular and developmental processes, yet a limited number of studies have been carried out on cell-growth regulation. Comprehensive studies at the transcriptional, proteomic and metabolic levels under defined controlled conditions are currently lacking.
This investigation shows public data taken from the BioInvestigation Index. (http://www.ebi.ac.uk/bioinvindex/study.seam?studyId=BII-S-1) Organism: Saccharomyces Cerevisiae
Submitter: Katy Wolstencroft
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Assay to measure gene expression of yeast saccharomyces cerevisiae under different nutrient limitations
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This data is public data taken from the BioInvestigation Index.
(http://www.ebi.ac.uk/bioinvindex/study.seam?studyId=BII-S-1)
Submitter: Katy Wolstencroft
Assay type: Transcriptional Profiling
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