COGEME_extraction_mod-chemostat_v1
Version 1

Biomass samples (20ml) were taken via the sample port of the Applikon fermenters. The cells were pelleted by centrifugation for 5min at 5000rpm. The supernatant was removed and the RNA pellet resuspended in the residual medium to form a slurry. This was added in a dropwise manner directly into a 5ml Teflon flask (B. Braun Biotech, Germany) containing liquid nitrogen and a 7mm-diameter tungsten carbide ball.

Taken from ArrayExpress: http://www.ebi.ac.uk/microarray-as/ae/browse.html?keywords=E-MEXP-115

SEEK ID: https://testing.sysmo-db.org/sops/15?version=1

Filename: COGEME_BII_1SOP.doc  Download

Format: Word document

Size: 25 KB

help Creators and Submitter
Activity

Views: 1398   Downloads: 66

Created: 3rd Dec 2009 at 11:24

Last updated: 5th Apr 2013 at 08:58

help Attributions

None

Version History

Version 1 (earliest) Created 3rd Dec 2009 at 11:24 by Katy Wolstencroft

No revision comments

Powered by
(v.1.15.0-pre)
Copyright © 2008 - 2024 The University of Manchester and HITS gGmbH