Detailed kinetic model for glycolysis of L. lactis. The model is basically the same model as that published by Hoefnagel et al 2002 (Mol. Biol. Reports 29, 157-161). The change made to the model is to make external glucose a variable such that a glucose pulse can be simulated.
Creators: Jacky Snoep, Ana Rute Neves
Submitter: Katy Wolstencroft
Model type: Ordinary differential equations (ODE)
Model format: SBML
Environment: Not specified
Organism: Lactococcus lactis
Studies: The effects of Redox Charge on Glycolytic Flux and 1 hidden item
Assays: Time dependent responses of glycolytic intermed... and 1 hidden item
Biomass samples (20ml) were taken via the sample port of the Applikon fermenters. The cells were pelleted by centrifugation for 5min at 5000rpm. The supernatant was removed and the RNA pellet resuspended in the residual medium to form a slurry. This was added in a dropwise manner directly into a 5ml Teflon flask (B. Braun Biotech, Germany) containing liquid nitrogen and a 7mm-diameter tungsten carbide ball.
Taken from ArrayExpress: http://www.ebi.ac.uk/microarray-as/ae/browse.html?keywords=E-MEXP-115 ...
Authors: Firstname Lastname, J Passarge, C A Reijenga, E Esgalhado, C C van der Weijden, M Schepper, M C Walsh, B M Bakker, K van Dam, H V Westerhoff, Firstname Lastname
Date Published: 22nd Aug 2000
Publication Type: Not specified
PubMed ID: 10951190