The effects of Redox Charge on Glycolytic Flux

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A study which combines mathematical modelling and experimental measurements to determine the effects of redox charge on glycolytic flux

SEEK ID: https://testing.sysmo-db.org/studies/6

Investigation: Glycolitic Flux in Lactococcus lactis

Projects: SysMO DB

Study position:

Experimentalists: Ana Rute Neves, Rita Ventura, Nahla Mansour, Claire Shearman, Michael J. Gasson, Christopher Maycock, Ana Ramos, and Helena Santos

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Creators and Submitter

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Submitter

Jacky Snoep

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Views: 1101

Created: 8th Aug 2011 at 13:50

Last updated: 10th Nov 2011 at 17:14

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People (1)
Programmes (1)
Projects (1)
Investigations (1)
Assays (1+1)
Data files (3)
Models (1)
Publications (1)

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Jacky Snoep

Projects: PSYSMO, MOSES, SysMOLAB, SulfoSys, SysMO DB

Institutions: Manchester Centre for Integrative Systems Biology, University of Manchester, University of Stellenbosch, HITS gGmbH

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Dummy programme

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Projects: SysMO DB

Web page: Not specified

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SysMO DB

The main objectives of SysMO-DB are to: facilitate the web-based exchange of data between research groups within- and inter- consortia, and to provide an integrated platform for the dissemination of the results of the SysMO projects to the scientific community. We aim to devise a progressive and scalable solution to the data management needs of the SysMO initiative, that:

maximises the ‘shelf life’ and utility of data generated by SysMO;
provides an integrated platform for the dissemination ...

Programme: Dummy programme

Public web page: http://www.sysmo-db.org/

Organisms: Lactic Acid Bacteria, Agapornis

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Glycolitic Flux in Lactococcus lactis

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Jacky Snoep

An investigation into the control mechanisms of glycolytic flux in Lactococcus lactis.

This investigation represents the data and models published by Hoefnagel et al in JWS Online and in M.H.N. Hoefnagel, J. Hugenholtz and J.L. SnoepMol. Biol. Reports 29, 157-161 (2002)

Submitter: Jacky Snoep

Studies: The effects of Redox Charge on Glycolytic Flux

Assays: Time dependent responses of glycolytic intermediates in a detailed glyco..., example assay

Snapshots: No snapshots

Created: 8th Aug 2011 at 13:47, Last updated: 10th Nov 2011 at 17:14

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Time dependent responses of glycolytic intermediates in a detailed glycolytic model of Lactococcus lactis during glucose run-out experiments

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Katy Wolstencroft

Detailed glycolytic model in Lactococcus lactis

Submitter: Katy Wolstencroft

Biological problem addressed: Metabolic Network

Investigation: Glycolitic Flux in Lactococcus lactis

Study: The effects of Redox Charge on Glycolytic Flux

Organisms: Lactococcus lactis

Models: Lactococcus lactis glycolysis

SOPs: No SOPs

Data files: Neves_timeSeries

Snapshots: No snapshots

Created: 8th Aug 2011 at 14:03, Last updated: 26th Sep 2017 at 10:54

1 hidden item

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Excell sheet template : concentrations of intracellular metabolites

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Olga Krebs

Excell sheet template : concentrations of intracellular metabolites

Creator: Olga Krebs

Submitter: Olga Krebs

Investigations: Glycolitic Flux in Lactococcus lactis, Growth control of the eukaryote cell: a systems..., Methods for interrelating 'omics data

Studies: Metabolomics data capture, The effects of Redox Charge on Glycolytic Flux, test study

Assays: Excel template for metabolomics data, test for biol problem and 1 hidden item

Created: 10th Jun 2009 at 17:35, Last updated: 26th Jun 2015 at 10:27

Neves_timeSeries

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Jacky Snoep

No description specified

Creator: Jacky Snoep

Submitter: Katy Wolstencroft

Investigations: Glycolitic Flux in Lactococcus lactis

Studies: The effects of Redox Charge on Glycolytic Flux

Assays: Time dependent responses of glycolytic intermed...

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Created: 17th Apr 2012 at 16:15, Last updated: 4th Feb 2013 at 14:00

Umlaut only in cells

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Stuart Owen

No description specified

Creator: Stuart Owen

Submitter: Stuart Owen

Investigations: Glycolitic Flux in Lactococcus lactis, Methods for interrelating 'omics data

Studies: Metabolomics data capture, The effects of Redox Charge on Glycolytic Flux

Assays: Excel template for metabolomics data and 1 hidden item

Created: 9th Mar 2012 at 09:34, Last updated: 9th Mar 2012 at 09:50

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Lactococcus lactis glycolysis

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Jacky Snoep

Detailed kinetic model for glycolysis of L. lactis. The model is basically the same model as that published by Hoefnagel et al 2002 (Mol. Biol. Reports 29, 157-161). The change made to the model is to make external glucose a variable such that a glucose pulse can be simulated.

Creators: Jacky Snoep, Ana Rute Neves

Submitter: Katy Wolstencroft

Model type: Ordinary differential equations (ODE)

Model format: SBML

Environment: Not specified

Organism: Lactococcus lactis

Investigations: Glycolitic Flux in Lactococcus lactis, inv1

Studies: The effects of Redox Charge on Glycolytic Flux and 1 hidden item

Assays: Time dependent responses of glycolytic intermed... and 1 hidden item

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Created: 19th Aug 2011 at 08:53, Last updated: 19th Aug 2011 at 08:59

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Is the glycolytic flux in Lactococcus lactis primarily controlled by the redox charge? Kinetics of NAD(+) and NADH pools determined in vivo by 13C NMR

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Abstract (Expand)

The involvement of nicotinamide adenine nucleotides (NAD(+), NADH) in the regulation of glycolysis in Lactococcus lactis was investigated by using (13)C and (31)P NMR to monitor in vivo the kinetics … of the pools of NAD(+), NADH, ATP, inorganic phosphate (P(i)), glycolytic intermediates, and end products derived from a pulse of glucose. Nicotinic acid specifically labeled on carbon 5 was synthesized and used in the growth medium as a precursor of pyridine nucleotides to allow for in vivo detection of (13)C-labeled NAD(+) and NADH. The capacity of L. lactis MG1363 to regenerate NAD(+) was manipulated either by turning on NADH oxidase activity or by knocking out the gene encoding lactate dehydrogenase (LDH). An LDH(-) deficient strain was constructed by double crossover. Upon supply of glucose, NAD(+) was constant and maximal (approximately 5 mm) in the parent strain (MG1363) but decreased abruptly in the LDH(-) strain both under aerobic and anaerobic conditions. NADH in MG1363 was always below the detection limit as long as glucose was available. The rate of glucose consumption under anaerobic conditions was 7-fold lower in the LDH(-) strain and NADH reached high levels (2.5 mm), reflecting severe limitation in regenerating NAD(+). However, under aerobic conditions the glycolytic flux was nearly as high as in MG1363 despite the accumulation of NADH up to 1.5 mm. Glyceraldehyde-3-phosphate dehydrogenase was able to support a high flux even in the presence of NADH concentrations much higher than those of the parent strain. We interpret the data as showing that the glycolytic flux in wild type L. lactis is not primarily controlled at the level of glyceraldehyde-3-phosphate dehydrogenase by NADH. The ATP/ADP/P(i) content could play an important role.

Authors: Ana Rute Neves, Rita Ventura, Nahla Mansour, Claire Shearman, Michael J Gasson, Christopher Maycock, Ana Ramos, Helena Santos

Date Published: 13th May 2002

Publication Type: Not specified

PubMed ID: 12011086

Citation:

Created: 8th Aug 2011 at 13:53, Last updated: 24th Mar 2022 at 10:39