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Biomass samples (20ml) were taken via the sample port of the Applikon fermenters. The cells were pelleted by centrifugation for 5min at 5000rpm. The supernatant was removed and the RNA pellet resuspended in the residual medium to form a slurry. This was added in a dropwise manner directly into a 5ml Teflon flask (B. Braun Biotech, Germany) containing liquid nitrogen and a 7mm-diameter tungsten carbide ball.
Taken from ArrayExpress: http://www.ebi.ac.uk/microarray-as/ae/browse.html?keywords=E-MEXP-115 ...
Creator: Katy Wolstencroft
Submitter: Katy Wolstencroft
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