output/qPCR_mesh_alas_JHBPTqMan_vATPaseE/20170407_alas.txt Version 1
_p_RNAinVAL/_I_01_LabTrials/_S_04_Stages/_A_04_stages-qPCR/
SEEK ID: https://testing.sysmo-db.org/data_files/1975?version=1
Filename:
output/qPCR_mesh_alas_JHBPTqMan_vATPaseE/20170407_alas.txt
Format: Plain text document
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Experiment: 04072017_RNAinVAL_stages-mesh_alas Selected Filter: FAM (483-533) Include Color Pos Name Cp Concentration Standard Status True 255 A13 Sample 13 29.87 4.68E1 0 True 255 A14 Sample 14 30.02 4.18E1 0 True 255 A15 Sample 15 33.19 1.27E0 0 E - Extrapolated concentration in standard curve True 255 A16 Sample 16 33.40 9.43E-1 0 E - Extrapolated concentration in standard curve True 255 A17 Sample 17 23.24 2.49E3 0 True 255 A18 Sample 18 23.33 2.36E3 0 True 255 A19 Sample 19 27.24 2.42E2 0 True 255 A20 Sample 20 27.19 2.49E2 0 True 255 A21 Sample 21 22.49 3.85E3 0 True 255 A22 Sample 22 22.70 3.41E3 0 True 255 A23 Sample 23 26.05 4.84E2 0 True 255 A24 Sample 24 26.08 4.75E2 0 True 255 B13 Sample 37 29.93 4.50E1 0 True 255 B14 Sample 38 29.30 7.12E1 0 True 255 B15 Sample 39 32.77 2.26E0 0 E - Extrapolated concentration in standard curve True 255 B16 Sample 40 32.60 2.80E0 0 E - Extrapolated concentration in standard curve True 255 B17 Sample 41 20.55 1.19E4 0 E - Extrapolated concentration in standard curve True 255 B18 Sample 42 20.67 1.11E4 0 E - Extrapolated concentration in standard curve True 255 B19 Sample 43 24.44 1.24E3 0 True 255 B20 Sample 44 24.34 1.31E3 0 True 255 B21 Sample 45 23.19 2.57E3 0 True 255 B22 Sample 46 23.17 2.59E3 0 True 255 B23 Sample 47 26.69 3.34E2 0 True 255 B24 Sample 48 26.59 3.53E2 0 True 255 C13 Sample 61 24.36 1.30E3 0 True 255 C14 Sample 62 24.29 1.35E3 0 True 255 C15 Sample 63 28.05 1.51E2 0 True 255 C16 Sample 64 27.81 1.74E2 0 True 255 C17 Sample 65 21.47 6.98E3 0 True 255 C18 Sample 66 21.24 8.00E3 0 True 255 C19 Sample 67 25.69 5.98E2 0 True 255 C20 Sample 68 25.58 6.35E2 0 True 255 C21 Sample 69 21.70 6.12E3 0 True 255 C22 Sample 70 21.70 6.09E3 0 True 255 C23 Sample 71 24.70 1.06E3 0 True 255 C24 Sample 72 24.84 9.80E2 0 True 255 D13 Sample 85 28.48 1.18E2 0 True 255 D14 Sample 86 28.14 1.43E2 0 True 255 D15 Sample 87 31.96 6.17E0 0 E - Extrapolated concentration in standard curve True 255 D16 Sample 88 32.11 5.17E0 0 E - Extrapolated concentration in standard curve True 255 D17 Sample 89 22.16 4.68E3 0 True 255 D18 Sample 90 22.21 4.55E3 0 True 255 D19 Sample 91 26.06 4.81E2 0 True 255 D20 Sample 92 26.33 4.12E2 0 True 255 D21 Sample 93 28.57 1.12E2 0 True 255 D22 Sample 94 28.58 1.11E2 0 True 255 D23 Sample 95 31.66 8.61E0 0 E - Extrapolated concentration in standard curve True 255 D24 Sample 96 31.27 1.31E1 0 True 255 E13 Sample 109 22.78 3.24E3 0 True 255 E14 Sample 110 22.67 3.47E3 0 True 255 E15 Sample 111 26.58 3.56E2 0 True 255 E16 Sample 112 26.58 3.55E2 0 True 255 E17 Sample 113 20.52 1.21E4 0 E - Extrapolated concentration in standard curve True 255 E18 Sample 114 20.58 1.17E4 0 E - Extrapolated concentration in standard curve True 255 E19 Sample 115 24.57 1.15E3 0 True 255 E20 Sample 116 24.62 1.11E3 0 True 255 E21 Sample 117 22.14 4.71E3 0 True 255 E22 Sample 118 22.14 4.73E3 0 True 255 E23 Sample 119 25.54 6.50E2 0 True 255 E24 Sample 120 25.80 5.58E2 0 True 255 F13 Sample 133 22.73 3.35E3 0 True 255 F14 Sample 134 22.93 2.99E3 0 True 255 F15 Sample 135 26.70 3.32E2 0 True 255 F16 Sample 136 26.61 3.50E2 0 True 255 F17 Sample 137 23.52 2.11E3 0 True 255 F18 Sample 138 23.52 2.11E3 0 True 255 F19 Sample 139 27.45 2.14E2 0 True 255 F20 Sample 140 27.49 2.10E2 0 True 255 F21 Sample 141 21.34 7.52E3 0 True 255 F22 Sample 142 21.65 6.29E3 0 True 255 F23 Sample 143 24.80 1.00E3 0 True 255 F24 Sample 144 24.60 1.13E3 0 True 255 G13 Sample 157 23.37 2.30E3 0 True 255 G14 Sample 158 23.36 2.32E3 0 True 255 G15 Sample 159 27.10 2.62E2 0 True 255 G16 Sample 160 27.32 2.31E2 0 True 255 G17 Sample 161 22.67 3.46E3 0 True 255 G18 Sample 162 22.61 3.59E3 0 True 255 G19 Sample 163 26.52 3.69E2 0 True 255 G20 Sample 164 26.54 3.64E2 0 True 255 G21 Sample 165 23.08 2.72E3 0 True 255 G22 Sample 166 23.44 2.22E3 0 True 255 G23 Sample 167 26.66 3.39E2 0 True 255 G24 Sample 168 26.59 3.52E2 0 True 255 H13 Sample 181 22.94 2.97E3 0 True 255 H14 Sample 182 22.92 3.01E3 0 True 255 H15 Sample 183 26.74 3.23E2 0 True 255 H16 Sample 184 26.79 3.14E2 0 True 255 H17 Sample 185 23.11 2.69E3 0 True 255 H18 Sample 186 23.12 2.67E3 0 True 255 H19 Sample 187 27.05 2.70E2 0 True 255 H20 Sample 188 27.08 2.65E2 0 True 255 I13 Sample 205 23.63 1.98E3 0 True 255 I14 Sample 206 23.28 2.43E3 0 True 255 I15 Sample 207 26.43 3.88E2 0 True 255 I16 Sample 208 26.78 3.16E2 0 True 255 I17 Sample 209 23.00 2.86E3 0 True 255 I18 Sample 210 22.99 2.88E3 0 True 255 I19 Sample 211 26.67 3.38E2 0 True 255 I20 Sample 212 26.45 3.83E2 0 True 255 J13 Sample 229 22.53 3.77E3 0 True 255 J14 Sample 230 22.33 4.23E3 0 True 255 J15 Sample 231 26.55 3.62E2 0 True 255 J16 Sample 232 26.37 4.01E2 0 True 255 J17 Sample 233 27.16 2.54E2 0 True 255 J18 Sample 234 27.26 2.39E2 0 True 255 J19 Sample 235 30.58 2.58E1 0 True 255 J20 Sample 236 30.82 2.07E1 0 True 255 K13 Sample 253 21.64 6.31E3 0 True 255 K14 Sample 254 22.12 4.77E3 0 True 255 K15 Sample 255 25.64 6.15E2 0 True 255 K16 Sample 256 25.69 5.96E2 0 True 255 K17 Sample 257 26.65 3.41E2 0 True 255 K18 Sample 258 26.80 3.13E2 0 True 255 K19 Sample 259 30.22 3.53E1 0 True 255 K20 Sample 260 30.13 3.81E1 0 True 255 L13 Sample 277 21.23 8.04E3 0 True 255 L14 Sample 278 21.19 8.23E3 0 True 255 L15 Sample 279 25.20 7.95E2 0 True 255 L16 Sample 280 25.21 7.91E2 0 True 255 L17 Sample 281 27.11 2.61E2 0 True 255 L18 Sample 282 26.96 2.84E2 0 True 255 L19 Sample 283 30.56 2.63E1 0 True 255 L20 Sample 284 30.69 2.33E1 0 True 255 M13 Sample 301 20.72 1.08E4 0 E - Extrapolated concentration in standard curve True 255 M14 Sample 302 21.02 9.08E3 0 True 255 M15 Sample 303 24.62 1.12E3 0 True 255 M16 Sample 304 24.77 1.02E3 0 True 255 M17 Sample 305 27.18 2.50E2 0 True 255 M18 Sample 306 27.18 2.51E2 0 True 255 M19 Sample 307 30.93 1.85E1 0 True 255 M20 Sample 308 30.94 1.83E1 0 True 255 N13 Sample 325 22.54 3.74E3 0 True 255 N14 Sample 326 21.91 5.39E3 0 True 255 N15 Sample 327 25.97 5.07E2 0 True 255 N16 Sample 328 26.10 4.71E2 0 True 255 N17 Sample 329 22.30 4.30E3 0 True 255 N18 Sample 330 22.27 4.37E3 0 True 255 N19 Sample 331 26.18 4.49E2 0 True 255 N20 Sample 332 26.10 4.71E2 0 True 65280 N23 Sample 335 0 True 65280 N24 Sample 336 0 True 255 O13 Sample 349 23.28 2.43E3 0 True 255 O14 Sample 350 23.58 2.04E3 0 True 255 O15 Sample 351 27.13 2.58E2 0 True 255 O16 Sample 352 27.20 2.48E2 0 True 255 O17 Sample 353 22.62 3.57E3 0 True 255 O18 Sample 354 22.63 3.55E3 0 True 255 O19 Sample 355 26.55 3.61E2 0 True 255 O20 Sample 356 26.65 3.41E2 0 True 128 O21 Sample 357 20.85 1.00E4 10000 True 128 O22 Sample 358 20.95 9.46E3 10000 True 128 O23 Sample 359 24.68 1.07E3 1000 True 128 O24 Sample 360 24.75 1.03E3 1000 True 255 P13 Sample 373 20.97 9.34E3 0 True 255 P14 Sample 374 20.68 1.11E4 0 E - Extrapolated concentration in standard curve True 255 P15 Sample 375 24.72 1.05E3 0 True 255 P16 Sample 376 24.91 9.42E2 0 True 255 P17 Sample 377 22.13 4.75E3 0 True 255 P18 Sample 378 22.07 4.92E3 0 True 255 P19 Sample 379 25.95 5.14E2 0 True 255 P20 Sample 380 25.95 5.12E2 0 True 128 P21 Sample 381 28.69 1.04E2 100 True 128 P22 Sample 382 28.90 9.16E1 100 True 128 P23 Sample 383 31.57 9.50E0 10 True 128 P24 Sample 384 31.48 1.05E1 10
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Created: 11th Nov 2021 at 19:59
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Version 1 (earliest) Created 11th Nov 2021 at 19:59 by Andrej Blejec
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https://orcid.org/0000-0001-7484-6031
Local data management; testing uploading/downloading using API
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Web page: Not specified
This is a demo project used for the demonstration of R package seekr functionality. R package seekr is based on SEEKR-API and enables batch upload of files organized in pISA-tree structure.
Programme: pISA-tree
Public web page: https://github.com/NIB-SI/seekr
Investigation: _I_01_LabTrials Short Name: 01_LabTrials Title: Laboratory trials Description: Selection of targets and their validation in trials performed in the laboratories and greenhouse at NIB Phenodata: ./phenodata_20210113.txt pISA Investigation creation date: 2021-01-13 pISA Investigation creator: Marko Petek Principal investigator: Marko Petek License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Andrej Blejec
Studies: _S_01_TargetSelect, _S_02_dsRNAorder, _S_03_dsRNAprod, _S_04_Stages, _S_05_jun2016, _S_06_oct2016, _S_07_dec2016, _S_08_jan2017, _S_09_jun2017, _S_10_apr2018, _S_11_may2018
Assays: _A_00_dsRNA_stabil-wet, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01_AgroRNA-wet, _A_01_LitData-dry, _A_01_pIsol-wet, _A_02-RNAisol, _A_02-RNAisol, _A_02-RNAisol, _A_02_UlrichTop100-BLAST, _A_02_plasmid-SangerSeq, _A_02_qPCR_ampl_test-wet, _A_03-qPCR, _A_03-qPCR, _A_03_RNaseItreat-wet, _A_03_patentDB-BLAST, _A_03_stages-RNAisol, _A_04_ortho-BLAST, _A_04_stages-qPCR, _A_05_CPB_gene-annot, _A_08_SelTargetsA-dry
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Submitter: Andrej Blejec
Investigation: _I_01_LabTrials-test
Assays: _A_02_qPCR_ampl_test-wet, _A_03_stages-RNAisol, _A_04_stages-qPCR
Assay: _A_04_stages-qPCR Short Name: 04_stages-qPCR Assay Class: WET Assay Type: qPCR Title: qPCR of stages samples Description: qPCR of stages samples for target genes pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Phenodata: ../../phenodata_20210113.txt Featuredata: Data: Lab manager: Kristina Gruden Assay chemistry: mixed Assay optimisation protocol (file path, link or citation): Pippeting: liquid handling station and manual Reaction volume: 5 ul qPCR platform: HT7900 ...
Submitter: Andrej Blejec
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_01_LabTrials-test
Study: _S_04_Stages
