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Study: _S_01_TargetSelect Short Name: 01_TargetSelect Title: Search for genes that might be good targets for control using dsRNAs (RNAi pesticides) Description: We will examine the results of RNAi screening experiments in model organisms (e.g. Drosophila melanogaster, Tribolium castaneum) as well as employ our previous data and literature knowledge on CPB gene expression and physiology Raw Data: pISA Study creation date: 2021-01-13 pISA Study creator: Marko Petek Principal investigator: Marko Petek License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
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Created: 11th Nov 2021 at 10:40
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Projects: testUpload, Test project 2019-10-30 11:13:00, Test project 2019-10-30 11:09:39, Test project 2019-11-02 19:40:30, Test project for pISA (104), _p_stRT, _p_Demo, Bla, Test project abcd, Test project 2021-07-18 22:00:39, Test project 2021-07-18 22:45:54, Test project 2021-07-18 23:08:33, Test project 2021-07-18 23:23:02, Test project 2021-07-21 00:08:04, Test project 2021-07-21 00:17:06, Test project 2021-07-21 00:18:25, Test project 2021-08-20 22:56:43, Testing of upload, _p_RNAinVAL
Institutions: National Institute of Biology, NIB, <script>alert('xss-institution-title')</script> & Test Institution
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Expertise: STatistics, R, Programming, Data Management
Local data management; testing uploading/downloading using API
Projects: testUpload, *, New project, New project 1, Test Project 7, Test Project, Test Project 8, Test Project 9, Test Project 10, Test Project 11, Test Project 12, Test Project 14, Test Project 13, Test Project 15, Test Project 16, Test Project 17, Test Project 18, Test: investigations 2019-10-30 11:00:08, Test: investigations 2019-10-30 11:02:00, Test project 2019-10-30 11:04:02, Test project 2019-10-30 11:08:47, Test project 2019-10-30 11:09:39, Test project 2019-10-30 11:10:03, Test project 2019-10-30 11:13:00, Test project 2019-11-01 11:41:43, Test project 2019-11-01 11:44:23, Test Project 20, Test project 21 2019-11-01 11:44:23, Test Project 21, Test project 23 2019-11-01 11:44:23, Test project 24 2019-11-01 11:44:23, Test project 33 2019-11-01 11:57:55, Test project 2019-11-01 12:01:35, Test project 40 2019-11-01 11:57:55, Test project 41 2019-11-01 11:57:55, Test project 42 2019-11-01 11:57:55, Test project 43 2019-11-01 11:57:55, Test project 44 2019-11-01 11:57:55, Test project 47 2019-11-01 11:57:55, Test project 48 2019-11-01 11:57:55, Test project 2019-11-01 19:36:07, Test project 2019-11-02 19:35:45, Test project 2019-11-02 19:40:30, Test project 50 2019-11-01 11:57:55, Test project 2019-11-02 19:47:45, Test project 51 2019-11-01 11:57:55, Test project 2019-11-02 20:22:36, Test project 2019-11-02 22:06:58, Test project 2019-11-02 22:10:04, Test project for pISA (104), _p_stRT, Test project 2019-11-13 21:46:46, Test project 2019-11-13 21:57:39, Test project 2019-11-13 21:59:51, Test project 2021-07-12 18:25:58, _p_Demo, Bla, Test project 2021-07-14 20:52:52, Test project 2021-07-14 20:54:23, Test project 2021-07-14 20:57:21, Test project 2021-07-15 23:13:46, Test project 2021-07-15 23:14:58, Test project 2021-07-15 23:16:29, Test project 2021-07-16 18:42:56, Test project 2021-07-16 18:43:56, Test project 2021-07-16 18:58:32, Test project 2021-07-16 19:00:28, Test project 2021-07-16 19:14:43, Test project 2021-07-16 19:17:03, Test project 2021-07-16 20:07:34, Test project 2021-07-17 20:31:23, Test project 2021-07-17 20:49:21, Test project abc, Test project abcd, Test project abcde, Test project 2021-07-18 21:54:46, Test project 2021-07-18 22:00:39, Test project 2021-07-18 23:08:33, Test project 2021-07-18 23:23:02, Test project 2021-07-21 00:08:04, Test project 2021-07-21 00:17:06, Test project 2021-07-21 00:18:25, Test project 2021-08-20 22:56:43, Testing of upload, _p_RNAinVAL
Web page: Not specified
This is a demo project used for the demonstration of R package seekr functionality. R package seekr is based on SEEKR-API and enables batch upload of files organized in pISA-tree structure.
Programme: pISA-tree
Public web page: https://github.com/NIB-SI/seekr
Organisms: Not specified
Investigation: _I_01_LabTrials Short Name: 01_LabTrials Title: Laboratory trials Description: Selection of targets and their validation in trials performed in the laboratories and greenhouse at NIB Phenodata: ./phenodata_20210113.txt pISA Investigation creation date: 2021-01-13 pISA Investigation creator: Marko Petek Principal investigator: Marko Petek License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Andrej Blejec
Studies: _S_01_TargetSelect, _S_02_dsRNAorder, _S_03_dsRNAprod, _S_04_Stages, _S_05_jun2016, _S_06_oct2016, _S_07_dec2016, _S_08_jan2017, _S_09_jun2017, _S_10_apr2018, _S_11_may2018
Assays: _A_00_dsRNA_stabil-wet, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01_AgroRNA-wet, _A_01_LitData-dry, _A_01_pIsol-wet, _A_02-RNAisol, _A_02-RNAisol, _A_02-RNAisol, _A_02_UlrichTop100-BLAST, _A_02_plasmid-SangerSeq, _A_02_qPCR_ampl_test-wet, _A_03-qPCR, _A_03-qPCR, _A_03_RNaseItreat-wet, _A_03_patentDB-BLAST, _A_03_stages-RNAisol, _A_04_ortho-BLAST, _A_04_stages-qPCR, _A_05_CPB_gene-annot, _A_08_SelTargetsA-dry
Snapshots: No snapshots
Assay: _A_05_CPB_gene-annot Short Name: 05_CPB_gene-annot Assay Class: DRY Assay Type: annot Title: CPB gene annotation using Blast2GO Description: Annotation of CPB predicted genes/proteins using Blast2GO (B2G) suite pISA Assay creation date: 2021-01-14 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data:
Submitter: Andrej Blejec
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_01_LabTrials-test
Study: _S_01_TargetSelect
Organisms: No organisms
SOPs: No SOPs
Data files: No Data files
Snapshots: No snapshots
Assay: _A_01_LitData-dry Short Name: 01_LitData-dry Assay Class: DRY Assay Type: dry Title: Compiling literature data on RNAi screens in insects Description: Compiling articles and final results of insect RNAi screens in model organisms (e.g. Drosophila melanogaster, Tribolium castaneum) pISA Assay creation date: 2021-01-13 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data:
Submitter: Andrej Blejec
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_01_LabTrials-test
Study: _S_01_TargetSelect
Organisms: No organisms
SOPs: No SOPs
Data files: output/Schnorrer_2010_Dm/Dm_lethal phenotypes n..., output/Tenerius_2011/Terenius 2011 mmc2.xls
Snapshots: No snapshots
Assay: _A_08_SelTargetsA-dry Short Name: 08_SelTargetsA-dry Assay Class: DRY Assay Type: dry Title: Bioinformatics analyses for individual selected target genes Description: In depth bioinformatic analysis for each target gene using various tools to predict possible off-target effects and select a fragment sequence for dsRNA synthesis pISA Assay creation date: 2021-01-14 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data:
Submitter: Andrej Blejec
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_01_LabTrials-test
Study: _S_01_TargetSelect
Organisms: No organisms
SOPs: No SOPs
Data files: output/JHBP/JHBP-like_dsRNA_region_splitter.tsv
Snapshots: No snapshots
Assay: _A_02_UlrichTop100-BLAST Short Name: 02_UlrichTop100-BLAST Assay Class: DRY Assay Type: BLAST Title: BLAST Ulrich top100 hits from Tribolium against CPB Description: Analysis of BLAST results from a query of top100 differentially expressed genes of Tribolium castaneum against Colorado potato beetle transcripts pISA Assay creation date: 2021-01-13 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data:
Submitter: Andrej Blejec
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_01_LabTrials-test
Study: _S_01_TargetSelect
Organisms: No organisms
SOPs: No SOPs
Data files: output/Tctop100cds__CPB_genome_nucl.xlsx
Snapshots: No snapshots
Assay: _A_04_ortho-BLAST Short Name: 04_ortho-BLAST Assay Class: DRY Assay Type: BLAST Title: BLAST to determine orthologs to model organisms Description: BLAST against D melanogaster and T castaneum to identify putative orthologous genes of CPB pISA Assay creation date: 2021-01-14 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data:
Submitter: Andrej Blejec
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_01_LabTrials-test
Study: _S_01_TargetSelect
Organisms: No organisms
SOPs: No SOPs
Data files: No Data files
Snapshots: No snapshots
Assay: _A_03_patentDB-BLAST Short Name: 03_patentDB-BLAST Assay Class: DRY Assay Type: BLAST Title: NCBI patent BLAST Description: This is to find CPB nucleotide and peptide sequences that are already protected by patents pISA Assay creation date: 2021-01-14 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data:
Submitter: Andrej Blejec
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_01_LabTrials-test
Study: _S_01_TargetSelect
Organisms: No organisms
SOPs: No SOPs
Data files: No Data files
Snapshots: No snapshots
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_02_UlrichTop100-BLAST/
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_01_LitData-dry/
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_08_SelTargetsA-dry/
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_01_LitData-dry/
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_05_CPB_gene-annot/
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_05_CPB_gene-annot/
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_05_CPB_gene-annot/
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_05_CPB_gene-annot/
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_05_CPB_gene-annot/
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_05_CPB_gene-annot/
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_05_CPB_gene-annot/
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_05_CPB_gene-annot/
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_05_CPB_gene-annot/
Creator: Andrej Blejec
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_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_05_CPB_gene-annot/
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_05_CPB_gene-annot/
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_05_CPB_gene-annot/
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_05_CPB_gene-annot/
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_05_CPB_gene-annot/
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_05_CPB_gene-annot/
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_05_CPB_gene-annot/
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_05_CPB_gene-annot/
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_05_CPB_gene-annot/
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_05_CPB_gene-annot/
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_05_CPB_gene-annot/
Creator: Andrej Blejec
Submitter: Andrej Blejec