_A_04_stages-qPCR
Single PageAssay: _A_04_stages-qPCR Short Name: 04_stages-qPCR Assay Class: WET Assay Type: qPCR Title: qPCR of stages samples Description: qPCR of stages samples for target genes pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Phenodata: ../../phenodata_20210113.txt Featuredata: Data: Lab manager: Kristina Gruden Assay chemistry: mixed Assay optimisation protocol (file path, link or citation): Pippeting: liquid handling station and manual Reaction volume: 5 ul qPCR platform: HT7900 Fast, ABI (SDS); Viia7, ABI (QuantStudio); LightCycler 480, Roche (LightCycler 480) qPCR protocol (file path, link or citation): https://dx.doi.org/10.3389%2Ffpls.2020.01250 One-step RT-PCR (RNA as input): No Well format: 384 MasterMix: FastStart Universal Probe Master (ROX), Roche (4914058001); TaqMan Universal PCR Master mix, ABI (4318157); Power SYBR® Green PCR Master Mix, ABI (4367659) No. sample technical replicates: 2 No. sample dilutions: 2 PCR controls used: NTC only No. standard curve dilutions (if using): 5 Operator: Marko Petek, Katja Stare Cq value determination software (if different from PCR platform): Quantification software: quantGenius qPCR quantification method: relative standard curve Notes:
SEEK ID: https://testing.sysmo-db.org/assays/805
Experimental assay
Projects: _p_Demo
Investigation: _I_01_LabTrials-test
Study: _S_04_Stages
Assay position:
Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: No organisms
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Created: 11th Nov 2021 at 10:40
Last updated: 11th Nov 2021 at 19:59
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Projects: testUpload, Test project 2019-10-30 11:13:00, Test project 2019-10-30 11:09:39, Test project 2019-11-02 19:40:30, Test project for pISA (104), _p_stRT, _p_Demo, Bla, Test project abcd, Test project 2021-07-18 22:00:39, Test project 2021-07-18 22:45:54, Test project 2021-07-18 23:08:33, Test project 2021-07-18 23:23:02, Test project 2021-07-21 00:08:04, Test project 2021-07-21 00:17:06, Test project 2021-07-21 00:18:25, Test project 2021-08-20 22:56:43, Testing of upload, _p_RNAinVAL
Institutions: National Institute of Biology, NIB, <script>alert('xss-institution-title')</script> & Test Institution
https://orcid.org/0000-0001-7484-6031
Local data management; testing uploading/downloading using API
Projects: testUpload, *, New project, New project 1, Test Project 7, Test Project, Test Project 8, Test Project 9, Test Project 10, Test Project 11, Test Project 12, Test Project 14, Test Project 13, Test Project 15, Test Project 16, Test Project 17, Test Project 18, Test: investigations 2019-10-30 11:00:08, Test: investigations 2019-10-30 11:02:00, Test project 2019-10-30 11:04:02, Test project 2019-10-30 11:08:47, Test project 2019-10-30 11:09:39, Test project 2019-10-30 11:10:03, Test project 2019-10-30 11:13:00, Test project 2019-11-01 11:41:43, Test project 2019-11-01 11:44:23, Test Project 20, Test project 21 2019-11-01 11:44:23, Test Project 21, Test project 23 2019-11-01 11:44:23, Test project 24 2019-11-01 11:44:23, Test project 33 2019-11-01 11:57:55, Test project 2019-11-01 12:01:35, Test project 40 2019-11-01 11:57:55, Test project 41 2019-11-01 11:57:55, Test project 42 2019-11-01 11:57:55, Test project 43 2019-11-01 11:57:55, Test project 44 2019-11-01 11:57:55, Test project 47 2019-11-01 11:57:55, Test project 48 2019-11-01 11:57:55, Test project 2019-11-01 19:36:07, Test project 2019-11-02 19:35:45, Test project 2019-11-02 19:40:30, Test project 50 2019-11-01 11:57:55, Test project 2019-11-02 19:47:45, Test project 51 2019-11-01 11:57:55, Test project 2019-11-02 20:22:36, Test project 2019-11-02 22:06:58, Test project 2019-11-02 22:10:04, Test project for pISA (104), _p_stRT, Test project 2019-11-13 21:46:46, Test project 2019-11-13 21:57:39, Test project 2019-11-13 21:59:51, Test project 2021-07-12 18:25:58, _p_Demo, Bla, Test project 2021-07-14 20:52:52, Test project 2021-07-14 20:54:23, Test project 2021-07-14 20:57:21, Test project 2021-07-15 23:13:46, Test project 2021-07-15 23:14:58, Test project 2021-07-15 23:16:29, Test project 2021-07-16 18:42:56, Test project 2021-07-16 18:43:56, Test project 2021-07-16 18:58:32, Test project 2021-07-16 19:00:28, Test project 2021-07-16 19:14:43, Test project 2021-07-16 19:17:03, Test project 2021-07-16 20:07:34, Test project 2021-07-17 20:31:23, Test project 2021-07-17 20:49:21, Test project abc, Test project abcd, Test project abcde, Test project 2021-07-18 21:54:46, Test project 2021-07-18 22:00:39, Test project 2021-07-18 23:08:33, Test project 2021-07-18 23:23:02, Test project 2021-07-21 00:08:04, Test project 2021-07-21 00:17:06, Test project 2021-07-21 00:18:25, Test project 2021-08-20 22:56:43, Testing of upload, _p_RNAinVAL
Web page: Not specified
This is a demo project used for the demonstration of R package seekr functionality. R package seekr is based on SEEKR-API and enables batch upload of files organized in pISA-tree structure.
Programme: pISA-tree
Public web page: https://github.com/NIB-SI/seekr
Investigation: _I_01_LabTrials Short Name: 01_LabTrials Title: Laboratory trials Description: Selection of targets and their validation in trials performed in the laboratories and greenhouse at NIB Phenodata: ./phenodata_20210113.txt pISA Investigation creation date: 2021-01-13 pISA Investigation creator: Marko Petek Principal investigator: Marko Petek License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Andrej Blejec
Studies: _S_01_TargetSelect, _S_02_dsRNAorder, _S_03_dsRNAprod, _S_04_Stages, _S_05_jun2016, _S_06_oct2016, _S_07_dec2016, _S_08_jan2017, _S_09_jun2017, _S_10_apr2018, _S_11_may2018
Assays: _A_00_dsRNA_stabil-wet, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01_AgroRNA-wet, _A_01_LitData-dry, _A_01_pIsol-wet, _A_02-RNAisol, _A_02-RNAisol, _A_02-RNAisol, _A_02_UlrichTop100-BLAST, _A_02_plasmid-SangerSeq, _A_02_qPCR_ampl_test-wet, _A_03-qPCR, _A_03-qPCR, _A_03_RNaseItreat-wet, _A_03_patentDB-BLAST, _A_03_stages-RNAisol, _A_04_ortho-BLAST, _A_04_stages-qPCR, _A_05_CPB_gene-annot, _A_08_SelTargetsA-dry
Study: _S_04_Stages Short Name: 04_Stages Title: Expression of target genes in CPB developmental stages and body parts Description: Expression of target genes in CPB developmental stages and body parts Raw Data: pISA Study creation date: 2021-05-10 pISA Study creator: Marko Petek Principal investigator: Marko Petek License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Andrej Blejec
Investigation: _I_01_LabTrials-test
Assays: _A_02_qPCR_ampl_test-wet, _A_03_stages-RNAisol, _A_04_stages-qPCR
_p_RNAinVAL/_I_01_LabTrials/_S_04_Stages/_A_04_stages-qPCR/
_p_RNAinVAL/_I_01_LabTrials/_S_04_Stages/_A_04_stages-qPCR/
_p_RNAinVAL/_I_01_LabTrials/_S_04_Stages/_A_04_stages-qPCR/
_p_RNAinVAL/_I_01_LabTrials/_S_04_Stages/_A_04_stages-qPCR/
_p_RNAinVAL/_I_01_LabTrials/_S_04_Stages/_A_04_stages-qPCR/
