_A_03-qPCR
Single PageAssay: _A_03-qPCR Short Name: 03-qPCR Assay Class: WET Assay Type: qPCR Title: qPCR for target genes Description: Quantitative PCR (qPCR) for selected target genes using the established methodology at NIB. pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Phenodata: ../../phenodata_20210113.txt Featuredata: Data: Lab manager: Kristina Gruden Assay chemistry: mixed Assay optimisation protocol (file path, link or citation): Pippeting: liquid handling station Reaction volume: 5 ul qPCR platform: HT7900 Fast, ABI (SDS) qPCR protocol (file path, link or citation): https://dx.doi.org/10.3389%2Ffpls.2020.01250 One-step RT-PCR (RNA as input): No Well format: 384 MasterMix: TaqMan Universal PCR Master mix, ABI (4318157); Power SYBR® Green PCR Master Mix, ABI (4367659) No. sample technical replicates: 2 No. sample dilutions: 2 PCR controls used: NTC only No. standard curve dilutions (if using): 5 Operator: Katja Stare Cq value determination software (if different from PCR platform): Quantification software: quantGenius qPCR quantification method: relative standard curve Notes:
SEEK ID: https://testing.sysmo-db.org/assays/823
Experimental assay
Projects: _p_Demo
Investigation: _I_01_LabTrials-test
Study: _S_07_dec2016
Assay position:
Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: No organisms
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Created: 11th Nov 2021 at 19:57
Last updated: 11th Nov 2021 at 19:57
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Projects: testUpload, Test project 2019-10-30 11:13:00, Test project 2019-10-30 11:09:39, Test project 2019-11-02 19:40:30, Test project for pISA (104), _p_stRT, _p_Demo, Bla, Test project abcd, Test project 2021-07-18 22:00:39, Test project 2021-07-18 22:45:54, Test project 2021-07-18 23:08:33, Test project 2021-07-18 23:23:02, Test project 2021-07-21 00:08:04, Test project 2021-07-21 00:17:06, Test project 2021-07-21 00:18:25, Test project 2021-08-20 22:56:43, Testing of upload, _p_RNAinVAL
Institutions: National Institute of Biology, NIB, <script>alert('xss-institution-title')</script> & Test Institution
https://orcid.org/0000-0001-7484-6031
Local data management; testing uploading/downloading using API
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Web page: Not specified
This is a demo project used for the demonstration of R package seekr functionality. R package seekr is based on SEEKR-API and enables batch upload of files organized in pISA-tree structure.
Programme: pISA-tree
Public web page: https://github.com/NIB-SI/seekr
Investigation: _I_01_LabTrials Short Name: 01_LabTrials Title: Laboratory trials Description: Selection of targets and their validation in trials performed in the laboratories and greenhouse at NIB Phenodata: ./phenodata_20210113.txt pISA Investigation creation date: 2021-01-13 pISA Investigation creator: Marko Petek Principal investigator: Marko Petek License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Andrej Blejec
Studies: _S_01_TargetSelect, _S_02_dsRNAorder, _S_03_dsRNAprod, _S_04_Stages, _S_05_jun2016, _S_06_oct2016, _S_07_dec2016, _S_08_jan2017, _S_09_jun2017, _S_10_apr2018, _S_11_may2018
Assays: _A_00_dsRNA_stabil-wet, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01_AgroRNA-wet, _A_01_LitData-dry, _A_01_pIsol-wet, _A_02-RNAisol, _A_02-RNAisol, _A_02-RNAisol, _A_02_UlrichTop100-BLAST, _A_02_plasmid-SangerSeq, _A_02_qPCR_ampl_test-wet, _A_03-qPCR, _A_03-qPCR, _A_03_RNaseItreat-wet, _A_03_patentDB-BLAST, _A_03_stages-RNAisol, _A_04_ortho-BLAST, _A_04_stages-qPCR, _A_05_CPB_gene-annot, _A_08_SelTargetsA-dry
Study: _S_07_dec2016 Short Name: 07_dec2016 Title: CPB feeding experiment dec2016 Description: Colorado potato beetle dsRNA feeding trial performed in December 2016 Raw Data: pISA Study creation date: 2021-05-10 pISA Study creator: Marko Petek Principal investigator: Marko Petek License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Andrej Blejec
Investigation: _I_01_LabTrials-test
Assays: _A_01-phenotyping, _A_02-RNAisol, _A_03-qPCR
_p_RNAinVAL/_I_01_LabTrials/_S_07_dec2016/_A_03-qPCR/
Creator: Andrej Blejec
Submitter: Andrej Blejec
