_A_02-RNAisol
Single PageAssay: _A_02-RNAisol Short Name: 02-RNAisol Assay Class: WET Assay Type: RNAisol Title: RNA isolation and pre-qPCR sample prep Description: RNA isolation and sample preparation for qPCR pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Lab manager: Marko Petek Phenodata: ../../phenodata_20210113.txt Featuredata: RNA ID: $_RNA Homogenisation protocol: handheld electric rotor-stator homogenization in tubes with Trizol Date Homogenisation: 2017-07-17 Isolation Protocol: ZymoRNA Date Isolation: 2017-07-17 Storage RNA: NA Dnase treatment protocol: Zymo DNase I Dnase ID: $_DNase Date DNAse_treatment: 2017-07-27 Storage_DNAse_treated: NA Operator: Marko Petek cDNA ID: $_cDNA DateRT: 2017-07-27 Notes: Data:
SEEK ID: https://testing.sysmo-db.org/assays/807
Experimental assay
Projects: _p_Demo
Investigation: _I_01_LabTrials-test
Study: _S_09_jun2017
Assay position:
Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: No organisms
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Created: 11th Nov 2021 at 10:40
Last updated: 11th Nov 2021 at 10:40
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Projects: testUpload, Test project 2019-10-30 11:13:00, Test project 2019-10-30 11:09:39, Test project 2019-11-02 19:40:30, Test project for pISA (104), _p_stRT, _p_Demo, Bla, Test project abcd, Test project 2021-07-18 22:00:39, Test project 2021-07-18 22:45:54, Test project 2021-07-18 23:08:33, Test project 2021-07-18 23:23:02, Test project 2021-07-21 00:08:04, Test project 2021-07-21 00:17:06, Test project 2021-07-21 00:18:25, Test project 2021-08-20 22:56:43, Testing of upload, _p_RNAinVAL
Institutions: National Institute of Biology, NIB, <script>alert('xss-institution-title')</script> & Test Institution
https://orcid.org/0000-0001-7484-6031
Local data management; testing uploading/downloading using API
Projects: testUpload, *, New project, New project 1, Test Project 7, Test Project, Test Project 8, Test Project 9, Test Project 10, Test Project 11, Test Project 12, Test Project 14, Test Project 13, Test Project 15, Test Project 16, Test Project 17, Test Project 18, Test: investigations 2019-10-30 11:00:08, Test: investigations 2019-10-30 11:02:00, Test project 2019-10-30 11:04:02, Test project 2019-10-30 11:08:47, Test project 2019-10-30 11:09:39, Test project 2019-10-30 11:10:03, Test project 2019-10-30 11:13:00, Test project 2019-11-01 11:41:43, Test project 2019-11-01 11:44:23, Test Project 20, Test project 21 2019-11-01 11:44:23, Test Project 21, Test project 23 2019-11-01 11:44:23, Test project 24 2019-11-01 11:44:23, Test project 33 2019-11-01 11:57:55, Test project 2019-11-01 12:01:35, Test project 40 2019-11-01 11:57:55, Test project 41 2019-11-01 11:57:55, Test project 42 2019-11-01 11:57:55, Test project 43 2019-11-01 11:57:55, Test project 44 2019-11-01 11:57:55, Test project 47 2019-11-01 11:57:55, Test project 48 2019-11-01 11:57:55, Test project 2019-11-01 19:36:07, Test project 2019-11-02 19:35:45, Test project 2019-11-02 19:40:30, Test project 50 2019-11-01 11:57:55, Test project 2019-11-02 19:47:45, Test project 51 2019-11-01 11:57:55, Test project 2019-11-02 20:22:36, Test project 2019-11-02 22:06:58, Test project 2019-11-02 22:10:04, Test project for pISA (104), _p_stRT, Test project 2019-11-13 21:46:46, Test project 2019-11-13 21:57:39, Test project 2019-11-13 21:59:51, Test project 2021-07-12 18:25:58, _p_Demo, Bla, Test project 2021-07-14 20:52:52, Test project 2021-07-14 20:54:23, Test project 2021-07-14 20:57:21, Test project 2021-07-15 23:13:46, Test project 2021-07-15 23:14:58, Test project 2021-07-15 23:16:29, Test project 2021-07-16 18:42:56, Test project 2021-07-16 18:43:56, Test project 2021-07-16 18:58:32, Test project 2021-07-16 19:00:28, Test project 2021-07-16 19:14:43, Test project 2021-07-16 19:17:03, Test project 2021-07-16 20:07:34, Test project 2021-07-17 20:31:23, Test project 2021-07-17 20:49:21, Test project abc, Test project abcd, Test project abcde, Test project 2021-07-18 21:54:46, Test project 2021-07-18 22:00:39, Test project 2021-07-18 23:08:33, Test project 2021-07-18 23:23:02, Test project 2021-07-21 00:08:04, Test project 2021-07-21 00:17:06, Test project 2021-07-21 00:18:25, Test project 2021-08-20 22:56:43, Testing of upload, _p_RNAinVAL
Web page: Not specified
This is a demo project used for the demonstration of R package seekr functionality. R package seekr is based on SEEKR-API and enables batch upload of files organized in pISA-tree structure.
Programme: pISA-tree
Public web page: https://github.com/NIB-SI/seekr
Investigation: _I_01_LabTrials Short Name: 01_LabTrials Title: Laboratory trials Description: Selection of targets and their validation in trials performed in the laboratories and greenhouse at NIB Phenodata: ./phenodata_20210113.txt pISA Investigation creation date: 2021-01-13 pISA Investigation creator: Marko Petek Principal investigator: Marko Petek License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Andrej Blejec
Studies: _S_01_TargetSelect, _S_02_dsRNAorder, _S_03_dsRNAprod, _S_04_Stages, _S_05_jun2016, _S_06_oct2016, _S_07_dec2016, _S_08_jan2017, _S_09_jun2017, _S_10_apr2018, _S_11_may2018
Assays: _A_00_dsRNA_stabil-wet, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01_AgroRNA-wet, _A_01_LitData-dry, _A_01_pIsol-wet, _A_02-RNAisol, _A_02-RNAisol, _A_02-RNAisol, _A_02_UlrichTop100-BLAST, _A_02_plasmid-SangerSeq, _A_02_qPCR_ampl_test-wet, _A_03-qPCR, _A_03-qPCR, _A_03_RNaseItreat-wet, _A_03_patentDB-BLAST, _A_03_stages-RNAisol, _A_04_ortho-BLAST, _A_04_stages-qPCR, _A_05_CPB_gene-annot, _A_08_SelTargetsA-dry
Study: _S_09_jun2017 Short Name: 09_jun2017 Title: CPB feeding experiment jun2017 Description: Colorado potato beetle dsRNA feeding trial performed in June 2017 Raw Data: pISA Study creation date: 2021-05-10 pISA Study creator: Marko Petek Principal investigator: Marko Petek License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Andrej Blejec
Investigation: _I_01_LabTrials-test
Assays: _A_00_dsRNA_stabil-wet, _A_01-phenotyping, _A_02-RNAisol, _A_03-qPCR
_p_RNAinVAL/_I_01_LabTrials/_S_09_jun2017/_A_02-RNAisol/
_p_RNAinVAL/_I_01_LabTrials/_S_09_jun2017/_A_02-RNAisol/
Creator: Andrej Blejec
Submitter: Andrej Blejec
