_p_RNAinVAL/_I_01_LabTrials/_S_01_TargetSelect/_A_08_SelTargetsA-dry/
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Created: 10th Nov 2021 at 11:25
Last updated: 10th Nov 2021 at 11:25
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Version 1 (earliest) Created 10th Nov 2021 at 11:25 by Andrej Blejec
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Projects: testUpload, Test project 2019-10-30 11:13:00, Test project 2019-10-30 11:09:39, Test project 2019-11-02 19:40:30, Test project for pISA (104), _p_stRT, _p_Demo, Bla, Test project abcd, Test project 2021-07-18 22:00:39, Test project 2021-07-18 22:45:54, Test project 2021-07-18 23:08:33, Test project 2021-07-18 23:23:02, Test project 2021-07-21 00:08:04, Test project 2021-07-21 00:17:06, Test project 2021-07-21 00:18:25, Test project 2021-08-20 22:56:43, Testing of upload, _p_RNAinVAL
Institutions: National Institute of Biology, NIB, <script>alert('xss-institution-title')</script> & Test Institution
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Test upload using seekr before upload to FAIRDOMHub
Programme: pISA-tree
Public web page: Not specified
Organisms: Not specified
Short Name: 01_LabTrials Title: Laboratory trials Description: Selection of targets and their validation in trials performed in the laboratories and greenhouse at NIB Phenodata: ./phenodata_20210113.txt pISA Investigation creation date: 2021-01-13 pISA Investigation creator: Marko Petek Principal investigator: Marko Petek License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Andrej Blejec
Studies: Investigation files, _S_01_TargetSelect, _S_02_dsRNAorder, _S_03_dsRNAprod, _S_04_Stages, _S_05_jun2016, _S_06_oct2016, _S_07_dec2016, _S_08_jan2017, _S_09_jun2017, _S_10_apr2018, _S_11_may2018
Assays: _A_00_Ecoli-dry, _A_00_dsRNA_stabil-wet, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01_AgroRNA-wet, _A_01_LitData-dry, _A_01_pIsol-wet, _A_02-RNAisol, _A_02-RNAisol, _A_02-RNAisol, _A_02_UlrichTop100-BLAST, _A_02_plasmid-SangerSeq, _A_02_qPCR_ampl_test-wet, _A_03-qPCR, _A_03-qPCR, _A_03-qPCR, _A_03_RNaseItreat-wet, _A_03_patentDB-BLAST, _A_03_stages-RNAisol, _A_04_ortho-BLAST, _A_04_prod-qPCR, _A_04_stages-qPCR, _A_05_CPB_gene-annot, _A_06_splitter_BLAST-dry, _A_07_MergeEvi-dry, _A_08_SelTargetsA-dry, _I_01_LabTrials-files, _S_01_TargetSelect-files, _S_02_dsRNAorder-files, _S_03_dsRNAprod-files, _S_04_Stages-files, _S_05_jun2016-files, _S_06_oct2016-files, _S_07_dec2016-files, _S_08_jan2017-files, _S_09_jun2017-files, _S_10_apr2018-files, _S_11_may2018-files
Snapshots: No snapshots
Short Name: 01_TargetSelect Title: Search for genes that might be good targets for control using dsRNAs (RNAi pesticides) Description: We will examine the results of RNAi screening experiments in model organisms (e.g. Drosophila melanogaster, Tribolium castaneum) as well as employ our previous data and literature knowledge on CPB gene expression and physiology Raw Data: pISA Study creation date: 2021-01-13 pISA Study creator: Marko Petek Principal investigator: Marko Petek License: CC BY 4.0 ...
Submitter: Andrej Blejec
Investigation: _I_01_LabTrials
Assays: _A_01_LitData-dry, _A_02_UlrichTop100-BLAST, _A_03_patentDB-BLAST, _A_04_ortho-BLAST, _A_05_CPB_gene-annot, _A_06_splitter_BLAST-dry, _A_07_MergeEvi-dry, _A_08_SelTargetsA-dry, _S_01_TargetSelect-files
Snapshots: No snapshots
Short Name: 08_SelTargetsA-dry Assay Class: DRY Assay Type: dry Title: Bioinformatics analyses for individual selected target genes Description: In depth bioinformatic analysis for each target gene using various tools to predict possible off-target effects and select a fragment sequence for dsRNA synthesis pISA Assay creation date: 2021-01-14 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data:
Submitter: Andrej Blejec
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_01_LabTrials
Study: _S_01_TargetSelect
Organisms: No organisms
SOPs: No SOPs
Data files: _ASSAY_METADATA.TXT, input/12864_2015_1880_MOESM1_ESM Top100 Tc RNAi..., other/NotInFinalSelection/Actin_genome-mRNA_Nuc..., other/NotInFinalSelection/smt3_dsRNA_region.txt, other/NotInFinalSelection/smt3_dsRNA_region_spl..., output/ALAS/Alas_blastn_Human_RefSeq_mRNA-HitTa..., output/ALAS/Alas_dsRNA_region.txt, output/ALAS/Alas_dsRNA_region_splitter.tsv, output/EGFP/Egfp_dsRNA_region.txt, output/EGFP/Egfp_dsRNA_region_splitter.tsv, output/JHBP/JHBP-like_blastn_Human_RefSeq_mRNA-..., output/JHBP/JHBP-like_dsRNA_region.txt, output/JHBP/JHBP-like_dsRNA_region_splitter.tsv, output/JHBP/JHBP-like_dsRNA_region_splitter_Ref..., output/MESH/Mesh_blastn_Human_RefSeq_mRNA-HitTa..., output/MESH/Mesh_mRNA.txt, output/MESH/Mesh_mRNA_splitter.tsv, reports/order_primers_dsRNA_syn/Order_primers&p..., reports/order_primers_dsRNA_syn/ordered_primers..., scripts/splitter_ablast_commands.txt
Snapshots: No snapshots