protocols/02CFG_Pos23_Permanent_culture.doc Version 1
_p_RNAinVAL/_I_01_LabTrials/_S_03_dsRNAprod/_A_01_pIsol-wet/
SEEK ID: https://testing.sysmo-db.org/documents/654?version=1
Filename:
protocols/02CFG_Pos23_Permanent_culture.doc
Format: Word document
Size: 30.5 KB
Export PNG
Creators and Submitter
Views: 385 Downloads: 4
Created: 10th Nov 2021 at 11:26
Last updated: 10th Nov 2021 at 11:26
AttributionsNone
Version History
Version 1 (earliest) Created 10th Nov 2021 at 11:26 by Andrej Blejec
No revision comments
Related items
Projects: testUpload, Test project 2019-10-30 11:13:00, Test project 2019-10-30 11:09:39, Test project 2019-11-02 19:40:30, Test project for pISA (104), _p_stRT, _p_Demo, Bla, Test project abcd, Test project 2021-07-18 22:00:39, Test project 2021-07-18 22:45:54, Test project 2021-07-18 23:08:33, Test project 2021-07-18 23:23:02, Test project 2021-07-21 00:08:04, Test project 2021-07-21 00:17:06, Test project 2021-07-21 00:18:25, Test project 2021-08-20 22:56:43, Testing of upload, _p_RNAinVAL
Institutions: National Institute of Biology, NIB, <script>alert('xss-institution-title')</script> & Test Institution
https://orcid.org/0000-0001-7484-6031
Local data management; testing uploading/downloading using API
Projects: testUpload, *, New project, New project 1, Test Project 7, Test Project, Test Project 8, Test Project 9, Test Project 10, Test Project 11, Test Project 12, Test Project 14, Test Project 13, Test Project 15, Test Project 16, Test Project 17, Test Project 18, Test: investigations 2019-10-30 11:00:08, Test: investigations 2019-10-30 11:02:00, Test project 2019-10-30 11:04:02, Test project 2019-10-30 11:08:47, Test project 2019-10-30 11:09:39, Test project 2019-10-30 11:10:03, Test project 2019-10-30 11:13:00, Test project 2019-11-01 11:41:43, Test project 2019-11-01 11:44:23, Test Project 20, Test project 21 2019-11-01 11:44:23, Test Project 21, Test project 23 2019-11-01 11:44:23, Test project 24 2019-11-01 11:44:23, Test project 33 2019-11-01 11:57:55, Test project 2019-11-01 12:01:35, Test project 40 2019-11-01 11:57:55, Test project 41 2019-11-01 11:57:55, Test project 42 2019-11-01 11:57:55, Test project 43 2019-11-01 11:57:55, Test project 44 2019-11-01 11:57:55, Test project 47 2019-11-01 11:57:55, Test project 48 2019-11-01 11:57:55, Test project 2019-11-01 19:36:07, Test project 2019-11-02 19:35:45, Test project 2019-11-02 19:40:30, Test project 50 2019-11-01 11:57:55, Test project 2019-11-02 19:47:45, Test project 51 2019-11-01 11:57:55, Test project 2019-11-02 20:22:36, Test project 2019-11-02 22:06:58, Test project 2019-11-02 22:10:04, Test project for pISA (104), _p_stRT, Test project 2019-11-13 21:46:46, Test project 2019-11-13 21:57:39, Test project 2019-11-13 21:59:51, Test project 2021-07-12 18:25:58, _p_Demo, Bla, Test project 2021-07-14 20:52:52, Test project 2021-07-14 20:54:23, Test project 2021-07-14 20:57:21, Test project 2021-07-15 23:13:46, Test project 2021-07-15 23:14:58, Test project 2021-07-15 23:16:29, Test project 2021-07-16 18:42:56, Test project 2021-07-16 18:43:56, Test project 2021-07-16 18:58:32, Test project 2021-07-16 19:00:28, Test project 2021-07-16 19:14:43, Test project 2021-07-16 19:17:03, Test project 2021-07-16 20:07:34, Test project 2021-07-17 20:31:23, Test project 2021-07-17 20:49:21, Test project abc, Test project abcd, Test project abcde, Test project 2021-07-18 21:54:46, Test project 2021-07-18 22:00:39, Test project 2021-07-18 23:08:33, Test project 2021-07-18 23:23:02, Test project 2021-07-21 00:08:04, Test project 2021-07-21 00:17:06, Test project 2021-07-21 00:18:25, Test project 2021-08-20 22:56:43, Testing of upload, _p_RNAinVAL
Web page: Not specified
Test upload using seekr before upload to FAIRDOMHub
Programme: pISA-tree
Public web page: Not specified
Short Name: 01_LabTrials Title: Laboratory trials Description: Selection of targets and their validation in trials performed in the laboratories and greenhouse at NIB Phenodata: ./phenodata_20210113.txt pISA Investigation creation date: 2021-01-13 pISA Investigation creator: Marko Petek Principal investigator: Marko Petek License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Andrej Blejec
Studies: Investigation files, _S_01_TargetSelect, _S_02_dsRNAorder, _S_03_dsRNAprod, _S_04_Stages, _S_05_jun2016, _S_06_oct2016, _S_07_dec2016, _S_08_jan2017, _S_09_jun2017, _S_10_apr2018, _S_11_may2018
Assays: _A_00_Ecoli-dry, _A_00_dsRNA_stabil-wet, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01-phenotyping, _A_01_AgroRNA-wet, _A_01_LitData-dry, _A_01_pIsol-wet, _A_02-RNAisol, _A_02-RNAisol, _A_02-RNAisol, _A_02_UlrichTop100-BLAST, _A_02_plasmid-SangerSeq, _A_02_qPCR_ampl_test-wet, _A_03-qPCR, _A_03-qPCR, _A_03-qPCR, _A_03_RNaseItreat-wet, _A_03_patentDB-BLAST, _A_03_stages-RNAisol, _A_04_ortho-BLAST, _A_04_prod-qPCR, _A_04_stages-qPCR, _A_05_CPB_gene-annot, _A_06_splitter_BLAST-dry, _A_07_MergeEvi-dry, _A_08_SelTargetsA-dry, _I_01_LabTrials-files, _S_01_TargetSelect-files, _S_02_dsRNAorder-files, _S_03_dsRNAprod-files, _S_04_Stages-files, _S_05_jun2016-files, _S_06_oct2016-files, _S_07_dec2016-files, _S_08_jan2017-files, _S_09_jun2017-files, _S_10_apr2018-files, _S_11_may2018-files
Short Name: 03_dsRNAprod Title: Production of selected desigend dsRNAs in-vivo Description: We established the production of two selected designed dsRNAs in-vivo in bacterial cultures of E. coli TH115 (DE3), a strain that does not produce RNases and is thus suitable for production of long dsRNA Raw Data: pISA Study creation date: 2021-01-14 pISA Study creator: Marko Petek Principal investigator: Marko Petek License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Andrej Blejec
Investigation: _I_01_LabTrials
Assays: _A_00_Ecoli-dry, _A_01_pIsol-wet, _A_02_plasmid-SangerSeq, _A_03_RNaseItreat-wet, _A_04_prod-qPCR, _S_03_dsRNAprod-files
Short Name: 01_pIsol-wet Assay Class: WET Assay Type: wet Title: Plasmid isolation from bacterial culture Description: Plasmids were obtained as bacterial culture from Addgene and isolated to be used for cloning pISA Assay creation date: 2021-01-14 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data:
Submitter: Andrej Blejec
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_01_LabTrials
Study: _S_03_dsRNAprod
