Assays

Assay: _A_03-qPCR Short Name: 03-qPCR Assay Class: WET Assay Type: qPCR Title: qPCR for target genes Description: Quantitative PCR (qPCR) for selected target genes using the established methodology at NIB. pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Phenodata: ../../phenodata_20210113.txt Featuredata: Data: Lab manager: Kristina Gruden Assay chemistry: mixed Assay optimisation protocol (file path, link or citation): Pippeting: liquid handling station Reaction volume: 5 ...

Assay: _A_04_ortho-BLAST Short Name: 04_ortho-BLAST Assay Class: DRY Assay Type: BLAST Title: BLAST to determine orthologs to model organisms Description: BLAST against D melanogaster and T castaneum to identify putative orthologous genes of CPB pISA Assay creation date: 2021-01-14 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data:

Assay: _A_00_dsRNA_stabil-wet Short Name: 00_dsRNA_stabil-wet Assay Class: WET Assay Type: wet Title: Stability testing of in-vitro synthetised dsRNA Description: Stability testing by gel electrophoresis before/after DNase and RNase reactions pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data:

Assay: _A_01-phenotyping Short Name: 01-phenotyping Assay Class: WET Assay Type: phenotyping Title: Measuring feeding trial parameters (using dsRNA produced in vivo in E coli) Description: Weight gain, survival, photos and other phenotypic observations pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek, Rok Ferenc Phenodata: ../../phenodata_20210113.txt Featuredata: Data:

Assay: _A_03-qPCR Short Name: 03-qPCR Assay Class: WET Assay Type: qPCR Title: qPCR for target genes Description: Quantitative PCR (qPCR) for selected target genes using the established methodology at NIB. pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Phenodata: ../../phenodata_20210113.txt Featuredata: Data: Lab manager: Kristina Gruden Assay chemistry: mixed Assay optimisation protocol (file path, link or citation): Pippeting: liquid handling station Reaction volume: 5 ...

Assay: _A_01_AgroRNA-wet Short Name: 01_AgroRNA-wet Assay Class: WET Assay Type: wet Title: Order dsRNA in-vitro synthesis at AgroRNA Description: Materials associated with the order of in-vitro synthesis of designed dsRNA, results of cleaning procedure and DNase I test to ensure the material is not DNA pISA Assay creation date: 2021-01-14 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data:

Assay: _A_03_RNaseItreat-wet Short Name: 03_RNaseItreat-wet Assay Class: WET Assay Type: wet Title: RNase If treatment of dsRNA Description: RNase If treatment of dsRNA produced in-vivo pISA Assay creation date: 2021-01-14 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data: