Assays

Short Name: 03_patentDB-BLAST Assay Class: DRY Assay Type: BLAST Title: NCBI patent BLAST Description: This is to find CPB nucleotide and peptide sequences that are already protected by patents pISA Assay creation date: 2021-01-14 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data:

Short Name: 01-phenotyping Assay Class: WET Assay Type: phenotyping Title: Measuring feeding trial parameters (using dsRNA produced in vivo in E coli) Description: Weight gain, survival, photos and other phenotypic observations pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek, Rok Ferenc Phenodata: ../../phenodata_20210113.txt Featuredata: Data:

Short Name: 03-qPCR Assay Class: WET Assay Type: qPCR Title: qPCR for target genes Description: Quantitative PCR (qPCR) for selected target genes using the established methodology at NIB. pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Phenodata: ../../phenodata_20210113.txt Featuredata: Data: Lab manager: Kristina Gruden Assay chemistry: mixed Assay optimisation protocol (file path, link or citation): Pippeting: liquid handling station Reaction volume: 5 ul qPCR platform: ...

Short Name: 02-RNAisol Assay Class: WET Assay Type: RNAisol Title: RNA isolation and pre-qPCR sample prep Description: RNA isolation and sample preparation for qPCR pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Lab manager: Marko Petek Phenodata: ../../phenodata_20210113.txt Featuredata: RNA ID: $_RNA Homogenisation protocol: handheld electric rotor-stator homogenization in tubes with Trizol Date Homogenisation: 2017-07-17 Isolation Protocol: ZymoRNA Date Isolation: 2017-07-17 ...

Short Name: 00_dsRNA_stabil-wet Assay Class: WET Assay Type: wet Title: Stability testing of in-vitro synthetised dsRNA Description: Stability testing by gel electrophoresis before/after DNase and RNase reactions pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data:

Short Name: 04_stages-qPCR Assay Class: WET Assay Type: qPCR Title: qPCR of stages samples Description: qPCR of stages samples for target genes pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Phenodata: ../../phenodata_20210113.txt Featuredata: Data: Lab manager: Kristina Gruden Assay chemistry: mixed Assay optimisation protocol (file path, link or citation): Pippeting: liquid handling station and manual Reaction volume: 5 ul qPCR platform: HT7900 Fast, ABI (SDS); Viia7, ABI ...

Short Name: 03_stages-RNAisol Assay Class: WET Assay Type: RNAisol Title: Expression of target genes in CPB developmental stages and body parts Description: Expression of target genes in CPB developmental stages and body parts - RNA isolation pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Lab manager: Marko Petek Phenodata: ../../phenodata_20210113.txt Featuredata: RNA ID: $_RNA Homogenisation protocol: handheld electric rotor-stator homogenization in tubes with Trizol Date ...