Intracellular metabolite measurements used for validation of the gycerol synthesis pathway model
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Created: 19th Aug 2011 at 11:30
Last updated: 12th Dec 2012 at 16:32
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Version 1 (earliest) Created 19th Aug 2011 at 11:30 by Katy Wolstencroft
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Projects: PSYSMO, MOSES, SysMOLAB, SulfoSys, SysMO DB
Institutions: Manchester Centre for Integrative Systems Biology, University of Manchester, University of Stellenbosch, HITS gGmbH
Projects: SysMO DB, Virtual Liver Network Developers
Institutions: School of Computer Science, University of Manchester, HITS gGmbH
Expertise: Biochemistry, Bioinformatics, Data Management
Tools: Data Management, Transcriptomics, Databases, Workflows, Web services, Taverna, Ontologies
I am a Research Fellow at the University of Manchester, working in Bioinformatics and Computer Science. I am also a guest researcher at the Vrije Universiteit.My research interests include scientific workflows, semantic discovery, and applying ontology technologies to biological data
The main objectives of SysMO-DB are to: facilitate the web-based exchange of data between research groups within- and inter- consortia, and to provide an integrated platform for the dissemination of the results of the SysMO projects to the scientific community. We aim to devise a progressive and scalable solution to the data management needs of the SysMO initiative, that:
- maximises the ‘shelf life’ and utility of data generated by SysMO;
- provides an integrated platform for the dissemination ...
Programme: Dummy programme
Public web page: http://www.sysmo-db.org/
Organisms: Lactic Acid Bacteria, Agapornis
Glycerol, a major by-product of ethanol fermentation by Saccharomyces cerevisiae, is of significant importance to the wine, beer, and ethanol production industries. This investigation aims to gain a clearer understanding of and to quantify the extent to which parameters of the pathway affect glycerol flux in S. cerevisiae.
This investigation represents the data and models submitted to JWS online by Cronwright et al (http://jjj.biochem.sun.ac.za/cgi-bin/getMoreInfo.py?modeldir=cronwright) and ...
Submitter: Katy Wolstencroft
Studies: Glycerol Synthesis Pathway
Assays: Extracellular Glycerol, Glycerol Synthesis Model construction, Intracellular Metabolites, fffff, test assay
Snapshots: No snapshots
This is a study of the glycerol synthesis pathway, using model predictions and experimental measurements
Submitter: Katy Wolstencroft
Investigation: Glycerol Synthesis in Saccharomyces cerevisiae
Assays: Extracellular Glycerol, Glycerol Synthesis Model construction, Intracellular Metabolites, test assay
Snapshots: No snapshots
A kinetic model of the glycerol synthesis pathway has been constructed. Kinetic parameters were collected from published values. Maximal enzyme activities and intracellular effector concentrations were determined experimentally.
Submitter: Katy Wolstencroft
Biological problem addressed: Metabolism
Investigation: Glycerol Synthesis in Saccharomyces cerevisiae
Study: Glycerol Synthesis Pathway
Organisms: Callimico goeldii
Models: Metabolic Control Analysis of Glycerol Synthesi...
SOPs: No SOPs
Data files: Cronwright Parameter Data, SysMO template
Snapshots: No snapshots
Intracellular metabolite concentrations were determined enzymatically by measuring the oxidation or reduction of NADH or NAD+, respectively, at 340 nm in a Beckman Coulter DU640 spectrophotometer
Submitter: Katy Wolstencroft
Assay type: Intracellular Metabolite Concentration
Technology type: Enzymatic Activity Measurements
Investigation: Glycerol Synthesis in Saccharomyces cerevisiae
Study: Glycerol Synthesis Pathway
Organisms: Callimico goeldii
SOPs: No SOPs
Data files: Cronwright Parameter Data
Snapshots: No snapshots