Studies

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Characterization of bacterial and fungal communities in switchgrass rhizosphere

The extent to which distinct bacterial endophyte communities occur between different plant organs and species is unclear, and has implications for bioprospecting efforts. Using V3 region of bacterial 16S rRNA gene, we investigated the diversity patterns in total, uncultured bacterial endophyte communities of three rainforest plant species, comparing leaf, stem, and root endophytes, plus rhizosphere soil community employing MiSeq. There was extensive overlap in bacterial communities between plant ...

Secondary metabolites like pyrrolizidine alkaloids (PAs) act as defense compounds against aboveground and belowground attackers. We studied to get a better understanding of relevance of microbial diversity in the rhizosphere for plant growth and behavior, specifically related to secondary metabolites production. We made soil dilution to compare the composition of soil microbial communities of soil suspension, incubation and rhizosphere soil by 16s rRNA high throughput pyrosequencing, as a ...