Assays

Short Name: 04_stages-qPCR Assay Class: WET Assay Type: qPCR Title: qPCR of stages samples Description: qPCR of stages samples for target genes pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Phenodata: ../../phenodata_20210113.txt Featuredata: Data: Lab manager: Kristina Gruden Assay chemistry: mixed Assay optimisation protocol (file path, link or citation): Pippeting: liquid handling station and manual Reaction volume: 5 ul qPCR platform: HT7900 Fast, ABI (SDS); Viia7, ABI ...

Short Name: 03_stages-RNAisol Assay Class: WET Assay Type: RNAisol Title: Expression of target genes in CPB developmental stages and body parts Description: Expression of target genes in CPB developmental stages and body parts - RNA isolation pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Lab manager: Marko Petek Phenodata: ../../phenodata_20210113.txt Featuredata: RNA ID: $_RNA Homogenisation protocol: handheld electric rotor-stator homogenization in tubes with Trizol Date ...

Short Name: 03_RNaseItreat-wet Assay Class: WET Assay Type: wet Title: RNase If treatment of dsRNA Description: RNase If treatment of dsRNA produced in-vivo pISA Assay creation date: 2021-01-14 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data:

Short Name: 00_Ecoli-dry Assay Class: DRY Assay Type: dry Title: E coli HT115 data Description: Information on E coli bacteria used for dsRNA production pISA Assay creation date: 2021-01-14 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data:

Short Name: 07_MergeEvi-dry Assay Class: DRY Assay Type: dry Title: Merge evidence from previous assays for CPB genome Description: Merge all evidence from literature, databases and BLAST results for target gene selection pISA Assay creation date: 2021-01-14 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data:

Short Name: 04_ortho-BLAST Assay Class: DRY Assay Type: BLAST Title: BLAST to determine orthologs to model organisms Description: BLAST against D melanogaster and T castaneum to identify putative orthologous genes of CPB pISA Assay creation date: 2021-01-14 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data:

Short Name: 02_UlrichTop100-BLAST Assay Class: DRY Assay Type: BLAST Title: BLAST Ulrich top100 hits from Tribolium against CPB Description: Analysis of BLAST results from a query of top100 differentially expressed genes of Tribolium castaneum against Colorado potato beetle transcripts pISA Assay creation date: 2021-01-13 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data: