Assays

Assay: _A_01_LitData-dry Short Name: 01_LitData-dry Assay Class: DRY Assay Type: dry Title: Compiling literature data on RNAi screens in insects Description: Compiling articles and final results of insect RNAi screens in model organisms (e.g. Drosophila melanogaster, Tribolium castaneum) pISA Assay creation date: 2021-01-13 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data:

Assay: _A_05_CPB_gene-annot Short Name: 05_CPB_gene-annot Assay Class: DRY Assay Type: annot Title: CPB gene annotation using Blast2GO Description: Annotation of CPB predicted genes/proteins using Blast2GO (B2G) suite pISA Assay creation date: 2021-01-14 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data:

Assay: _A_01-phenotyping Short Name: 01-phenotyping Assay Class: WET Assay Type: phenotyping Title: Measuring feeding trial parameters Description: Hatching, weight gain, survival, photos and other phenotypic observations pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Phenodata: ../../phenodata_20210113.txt Featuredata: Data:

Assay: _A_01_pIsol-wet Short Name: 01_pIsol-wet Assay Class: WET Assay Type: wet Title: Plasmid isolation from bacterial culture Description: Plasmids were obtained as bacterial culture from Addgene and isolated to be used for cloning pISA Assay creation date: 2021-01-14 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data:

Assay: _A_02-RNAisol Short Name: 02-RNAisol Assay Class: WET Assay Type: RNAisol Title: RNA isolation and pre-qPCR sample prep Description: RNA isolation and sample preparation for qPCR pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Lab manager: Marko Petek Phenodata: ../../phenodata_20210113.txt Featuredata: RNA ID: $_RNA Homogenisation protocol: handheld electric rotor-stator homogenization in tubes with Trizol Date Homogenisation: 2017-08-17 Isolation Protocol: ZymoRNA ...

Assay: _A_02_qPCR_ampl_test-wet Short Name: 02_qPCR_ampl_test-wet Assay Class: WET Assay Type: wet Title: Test of qPCR amplicons Description: Test of qPCR ampliconed designed to follow expression of target genes pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Phenodata: None Featuredata: Data:

Assay: _A_02-RNAisol Short Name: 02-RNAisol Assay Class: WET Assay Type: RNAisol Title: RNA isolation and pre-qPCR sample prep Description: RNA isolation and sample preparation for qPCR pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Lab manager: Marko Petek Phenodata: ../../phenodata_20210113.txt Featuredata: RNA ID: $_RNA Homogenisation protocol: handheld electric rotor-stator homogenization in tubes with Trizol Date Homogenisation: 2017-04-26 Isolation Protocol: ZymoRNA ...