output/pPCR_RP4_JHBP_SYBR/qPCR_RNAinVAL

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																																	ORIG. SORT	SORT	Well Position	sample name	RP4	JHBP	Rel quant
	RP4												JHBP																				1	1	A1	egg early 3	17.3	30.17	100000
	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24									2	2	A2	egg early 3	17.42	30.62	100000
A	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x									3	3	A3	egg early 3	21.67	34.97	10000
B	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x									4	4	A4	egg early 3	21.62	34.84	10000
C	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x									13	5	B1	egg early 4	17.18	30.66	100000
D	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	lukenj/amplikon:		160		(2 x 2 x 39 vz)				14	6	B2	egg early 4	17.25	30.97	100000
E	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	NTC, umeritvena:		10						15	7	B3	egg early 4	21.34		10000
F	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	procent izgub:		20	%					16	8	B4	egg early 4	21.22	34.15	10000
G	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	10x	10x	100x	100x	št. reakcij z upoštevano izgubo:		204						25	9	C1	eggs early 2	15.68	26.71	100000
H	10x	10x	100x	100x	10x	10x	100x	100x	NTC	NTC	NTC	NTC	10x	10x	100x	100x	10x	10x	100x	100x	NTC	NTC	NTC	NTC									26	10	C2	eggs early 2	15.82	27.07	100000
I	10x	10x	100x	100x	10x	10x	100x	100x					10x	10x	100x	100x	10x	10x	100x	100x													27	11	C3	eggs early 2	19.49	28.96	10000
J	10x	10x	100x	100x	10x	10x	100x	100x					10x	10x	100x	100x	10x	10x	100x	100x													28	12	C4	eggs early 2	19.53	28.88	10000
K	10x	10x	100x	100x	10x	10x	100x	100x					10x	10x	100x	100x	10x	10x	100x	100x													37	13	D1	eggs early 3	18.15	28.93	100000
L	10x	10x	100x	100x	10x	10x	100x	100x					10x	10x	100x	100x	10x	10x	100x	100x													38	14	D2	eggs early 3	18.15	29.36	100000
M	10x	10x	100x	100x	10x	10x	100x	100x					10x	10x	100x	100x	10x	10x	100x	100x					Syber Green	JHBP, LdRP4							39	15	D3	eggs early 3	22.36	33.19	10000
N	10x	10x	100x	100x	10x	10x	100x	100x			NTC	NTC	10x	10x	100x	100x	10x	10x	100x	100x			NTC	NTC									40	16	D4	eggs early 3	22.53	33.17	10000
O	10x	10x	100x	100x	10x	10x	100x	100x	10	10	100	100	10x	10x	100x	100x	10x	10x	100x	100x	10	10	100	100	no.:	204							49	17	E1	egg late 1	15.62	16.67	100000
P	10x	10x	100x	100x	10x	10x	100x	100x	1000	1000	10k	10k	10x	10x	100x	100x	10x	10x	100x	100x	1000	1000	10k	10k	FP:	300 nM	0.05						50	18	E2	egg late 1	15.8	16.72	100000
																									RP:	300 nM	0.05						51	19	E3	egg late 1	19.55	20.46	10000
																																	52	20	E4	egg late 1	19.39	20.51	10000
																																	61	21	F1	egg late 2	15.98	17.76	100000
	redčenje cDNA pool-a za umeritveno krivuljo: -ročno 90 sigma dH2O + 10 POOL cDNA (od 2016)																								UMM (2x)	510							62	22	F2	egg late 2	15.95	17.78	100000
	redčenje cDNA vzorcev: - na robotu 45+5 ul; 2 redčini na isto plato																								FP	10.200000000000001			MasterMix used:				63	23	F3	egg late 2	19.7	21.83	10000
																									RP	10.200000000000001			ABI SYBR power				64	24	F4	egg late 2	19.72	21.83	10000
																									water	81.59999999999991							73	25	G1	egg late 3	16.13	17.3	100000
																									sample	408							74	26	G2	egg late 3	16.27	17.53	100000
																									sum	1020							75	27	G3	egg late 3	20.14	21.51	10000
																									pipet	3							76	28	G4	egg late 3	20.07	21.6	10000
																																	85	29	H1	neonatal 1	17.06	17.58	100000
																																	86	30	H2	neonatal 1	17.01	17.56	100000
																																	87	31	H3	neonatal 1	21.03	21.71	10000
																																	88	32	H4	neonatal 1	21.01	21.7	10000
																																	93	33	I1	neonatal 2	16.89	18.64	100000
																																	94	34	I2	neonatal 2	16.92	18.65	100000
																									primerji:								95	35	I3	neonatal 2	19.91	21.85	10000
																									Stock	Delovne raztopine za 5 ul reakcije:							96	36	I4	neonatal 2	19.96	21.9	10000
																									Primerji		priprava	uM	ul v mix	V reakcije	konc/reakcijo	nM	101	37	J1	neonatal 3	16.1	16.88	100000
																									100 uM	30 uM	30+70	30	0.15	5	0.9	900	102	38	J2	neonatal 3	16.05	16.95	100000
																												30	0.09999999999999999	5	0.6	599.9999999999999	103	39	J3	neonatal 3	20.09	21.29	10000
																												30	0.049999999999999996	5	0.3	299.99999999999994	104	40	J4	neonatal 3	20.11	21.3	10000
																									Sonda								109	41	K1	2nd larva 2	17.17	15.33	100000
																									100 uM	10 uM	10+90	10	0.125	5	0.25	250	110	42	K2	2nd larva 2	17.03	15.49	100000
																												10	0.075	5	0.15	150	111	43	K3	2nd larva 2	20.98	19.74	10000
																																	112	44	K4	2nd larva 2	20.98	19.77	10000
																																	117	45	L1	2nd larva 3	16.05	14.17	100000
																																	118	46	L2	2nd larva 3	16.01	14.33	100000
																																	119	47	L3	2nd larva 3	19.9	18.39	10000
																																	120	48	L4	2nd larva 3	19.99	18.47	10000
																																	125	49	M1	2nd larva 4	16.37	14.52	100000
																																	126	50	M2	2nd larva 4	16.33	14.59	100000
																																	127	51	M3	2nd larva 4	20.19	18.62	10000
																																	128	52	M4	2nd larva 4	20.18	18.62	10000
																																	133	53	N1	2nd larva 5	16.35	15.18	100000
																																	134	54	N2	2nd larva 5	16.27	15.23	100000
																																	135	55	N3	2nd larva 5	20.22	19.33	10000
																																	136	56	N4	2nd larva 5	20.18	19.3	10000
																																	143	57	O1	3rd larva 1	18.15	16.02	100000
																																	144	58	O2	3rd larva 1	18.12	16.04	100000
																																	145	59	O3	3rd larva 1	22.13	19.99	10000
																																	146	60	O4	3rd larva 1	22.17	20	10000
																																	155	61	P1	3rd larva 2	16.74	13.99	100000
																																	156	62	P2	3rd larva 2	16.73	14.12	100000
																																	157	63	P3	3rd larva 2	20.67	18.12	10000
																																	158	64	P4	3rd larva 2	20.66	18.13	10000
																																	5	65	A5	3rd larva 3	17.62	15.92	100000
																																	6	66	A6	3rd larva 3	17.26	15.98	100000
																																	7	67	A7	3rd larva 3	21.52	20.07	10000
																																	8	68	A8	3rd larva 3	21.52	20.06	10000
																																	17	69	B5	4th early larva 1	16.11	13.87	100000
																																	18	70	B6	4th early larva 1	17.31	13.81	100000
																																	19	71	B7	4th early larva 1	20.78	17.86	10000
																																	20	72	B8	4th early larva 1	20.73	17.84	10000
																																	29	73	C5	4th early larva 2	16.9	14.34	100000
																																	30	74	C6	4th early larva 2	17.82	14.27	100000
																																	31	75	C7	4th early larva 2	21.9	18.73	10000
																																	32	76	C8	4th early larva 2	21.84	18.72	10000
																																	41	77	D5	4th early larva 3	16.58	17.57	100000
																																	42	78	D6	4th early larva 3	16.05	17.67	100000
																																	43	79	D7	4th early larva 3	20.28	21.93	10000
																																	44	80	D8	4th early larva 3	20.27	21.95	10000
																																	53	81	E5	4th early larva 4	17.07	13.92	100000
																																	54	82	E6	4th early larva 4	16.69	13.98	100000
																																	55	83	E7	4th early larva 4	20.76	17.92	10000
																																	56	84	E8	4th early larva 4	20.8	17.99	10000
																																	65	85	F5	4th late larva 1	17.77	16.65	100000
																																	66	86	F6	4th late larva 1	17.77	16.62	100000
																																	67	87	F7	4th late larva 1	21.73	20.66	10000
																																	68	88	F8	4th late larva 1	21.77	20.8	10000
																																	77	89	G5	4th late larva 2	18.27	16.13	100000
																																	78	90	G6	4th late larva 2	18.17	16.2	100000
																																	79	91	G7	4th late larva 2	22.24	20.54	10000
																																	80	92	G8	4th late larva 2	22.2	20.52	10000
																																	89	93	H5	4th late larva 3	18.62	15.38	100000
																																	90	94	H6	4th late larva 3	18.63	15.41	100000
																																	91	95	H7	4th late larva 3	22.32	19.47	10000
																																	92	96	H8	4th late larva 3	22.36	19.37	10000
																																	97	97	I5	4th late larva 4	16.57	14.49	100000
																																	98	98	I6	4th late larva 4	17.26	14.32	100000
																																	99	99	I7	4th late larva 4	20.76	18.13	10000
																																	100	100	I8	4th late larva 4	20.73	18.06	10000
																																	105	101	J5	pupa 1	18.54	30.84	100000
																																	106	102	J6	pupa 1	19.02	29.86	100000
																																	107	103	J7	pupa 1	22.38	32.11	10000
																																	108	104	J8	pupa 1	22.34	33.66	10000
																																	113	105	K5	pupa 2	18.75	29.49	100000
																																	114	106	K6	pupa 2	18.24	29.54	100000
																																	115	107	K7	pupa 2	21.98	37.08	10000
																																	116	108	K8	pupa 2	22		10000
																																	121	109	L5	pupa 3	18.37	30.17	100000
																																	122	110	L6	pupa 3	18.19	30.27	100000
																																	123	111	L7	pupa 3	21.84	33.9	10000
																																	124	112	L8	pupa 3	21.82	33.5	10000
																																	129	113	M5	pupa 4	19.99	30.95	100000
																																	130	114	M6	pupa 4	19.68	30.84	100000
																																	131	115	M7	pupa 4	23.64	32.88	10000
																																	132	116	M8	pupa 4	23.59	32.27	10000
																																	137	117	N5	adult 1	18.03	14.69	100000
																																	138	118	N6	adult 1	17.99	14.69	100000
																																	139	119	N7	adult 1	21.76	18.52	10000
																																	140	120	N8	adult 1	21.72	18.55	10000
																																	147	121	O5	adult 2	17.19	14.85	100000
																																	148	122	O6	adult 2	17.08	14.85	100000
																																	149	123	O7	adult 2	20.81	18.81	10000
																																	150	124	O8	adult 2	20.85	18.75	10000
																																	159	125	P5	adult 3	18.61	13.87	100000
																																	160	126	P6	adult 3	18.48	13.85	100000
																																	161	127	P7	adult 3	22.06	17.65	10000
																																	162	128	P8	adult 3	22.04	17.64	10000
																																	9	129	A9	adult 4	17.53	15.56	100000
																																	10	130	A10	adult 4	17.59	15.62	100000
																																	11	131	A11	adult 4	20.58	19.02	10000
																																	12	132	A12	adult 4	21.16	18.98	10000
																																	21	133	B9	foregut	18	19.25	100000
																																	22	134	B10	foregut	18.03	19.24	100000
																																	23	135	B11	foregut	21.6	23.46	10000
																																	24	136	B12	foregut	21.83	23.37	10000
																																	33	137	C9	midgut	17.91	14.78	100000
																																	34	138	C10	midgut	17.9	14.79	100000
																																	35	139	C11	midgut	21.55	18.55	10000
																																	36	140	C12	midgut	21.58	18.72	10000
																																	45	141	D9	hindgut	23.28	28.99	100000
																																	46	142	D10	hindgut	23.24	29.12	100000
																																	47	143	D11	hindgut	26.72	32.76	10000
																																	48	144	D12	hindgut	26.68	33.13	10000
																																	57	145	E9	haemolymph	17.96	17.03	100000
																																	58	146	E10	haemolymph	17.88	16.92	100000
																																	59	147	E11	haemolymph	21.6	20.97	10000
																																	60	148	E12	haemolymph	21.66	20.93	10000
																																	69	149	F9	carcass	17.33	14.37	100000
																																	70	150	F10	carcass	17.05	14.29	100000
																																	71	151	F11	carcass	20.73	18.05	10000
																																	72	152	F12	carcass	20.74	17.94	10000
																																	81	153	G9	legs	18.58	15.15	100000
																																	82	154	G10	legs	18.57	15.09	100000
																																	83	155	G11	legs	22.39	19.16	10000
																																	84	156	G12	legs	22.28	19.29	10000
																																	151	157	O21	STD_10x	17.55	15.27	1000000	-3.8049999999999997	-4.095000000000001
																																	152	158	O22	STD_10x	17.57	15.32	1000000
																																	153	159	O23	STD_100x	21.26	19.46	100000	-3.844999999999999	-3.7300000000000004
																																	154	160	O24	STD_100x	21.47	19.32	100000
																																	163	161	P21	STD_1000x	25.15	23.12	10000	-3.035	-3.1549999999999976
																																	164	162	P22	STD_1000x	25.27	23.12	10000
																																	165	163	P23	STD_10kx	28.32	26.18	1000
																																	166	164	P24	STD_10kx	28.17	26.37	1000
																																	141	165	N23	NTC
																																	142	166	N24	NTC