Selected Cell
Cell:
Value:
RNAinVAL_JUN2017_exp
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| RUN ON ABI 9600 MACHINE | |||||||||||||||||||||||||||||||||||
| amplikon | JHBP_TqMn | alas | vATPaseE | ||||||||||||||||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | ||||||||||||
| A | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | |||||||||||
| B | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | |||||||||||
| C | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | vzorcev/amplikon: | 24 | |||||||||
| D | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | lukenj/vz: | 4 | |||||||||
| E | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | NTC, umeritvena: | 10 | |||||||||
| F | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | procent izgub: | 20 | % | ||||||||
| G | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | št. reakcij z upoštevano izgubo: | 127.2 | |||||||||
| H | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | |||||||||||
| I | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | |||||||||||||||||||||||
| J | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | |||||||||||||||||||||||
| K | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | |||||||||||||||||||||||
| L | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | RNAinVAL škatla | ||||||||||||||||||||||
| M | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | TaqMan amplikoni | ||||||||||||||||||||||
| N | 10x | 10x | 100x | 100x | NTC | NTC | 10x | 10x | 100x | 100x | NTC | NTC | 10x | 10x | 100x | 100x | NTC | NTC | št. reakcij: | 128 | |||||||||||||||
| O | 10x | 10x | 100x | 100x | 10 | 10 | 100 | 100 | 10x | 10x | 100x | 100x | 10 | 10 | 100 | 100 | 10x | 10x | 100x | 100x | 10 | 10 | 100 | 100 | sonda: | 150 | 0.075 | MasterMix used: | |||||||
| P | 10x | 10x | 100x | 100x | 1000 | 1000 | 10k | 10k | 10x | 10x | 100x | 100x | 1000 | 1000 | 10k | 10k | 10x | 10x | 100x | 100x | 1000 | 1000 | 10k | 10k | FP: | 300 | 0.05 | Roche Probes Master with ROX | |||||||
| RP: | 300 | 0.05 | SYBR Power Abi | ||||||||||||||||||||||||||||||||
| 1) | za vzorce pripraviti redčine 10x, 100x, 1000x, 10kx | ||||||||||||||||||||||||||||||||||
| MIX for each Taqman amplicon | 18S assay: | MIX for each SYBR amplicon | |||||||||||||||||||||||||||||||||
| 2) | pool redčit 10x, 100x, 1000x, 10 000x | UMM (2x) | 320 | ul | 320 | ul | 320 | ul | |||||||||||||||||||||||||||
| RUN ON ROCHE LC480 MACHINE | FP | 6.4 | ul | 32 | ul | 6.4 | ul | ||||||||||||||||||||||||||||
| amplikon | JHBP_SYBR | LdRP4_SYBR | CONTIG33_SYBR (LdSmt3) | RP | 6.4 | ul | / | ul | 6.4 | ul | |||||||||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | sonda | 9.6 | ul | / | ul | / | ul | |||||
| A | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | ddH2O | 41.60000000000002 | ul | 32 | ul | 51.200000000000045 | ul | ||||
| B | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | vzorci | 256 | 256 | 256 | |||||||
| C | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | skupaj mixa | 384 | ul | 384 | ul | 384 | ul | ||||
| D | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | pipetiramo | 3 | ul | 3 | ul | 3 | ul | ||||
| E | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | skupaj Mix+Vz | 640 | ul | 640 | ul | 640 | ul | ||||
| F | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | ostane mixa | 66 | ul | 66 | ul | 66 | ul | ||||
| G | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | |||||||||||
| H | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | |||||||||||
| I | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | primerji: | ||||||||||||||||||||||
| J | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | Stock | Delovne raztopine za 5 ul reakcije: | |||||||||||||||||||||
| K | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | Primerji | priprava | uM | ul v mix | V reakcije | konc/reakcijo | nM | ||||||||||||||||
| L | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 100 uM | 30 uM | 30+70 | 30 | 0.15 | 5 | 0.9 | 900 | |||||||||||||||
| M | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 10x | 10x | 100x | 100x | 30 | 0.09999999999999999 | 5 | 0.6 | 599.9999999999999 | ||||||||||||||||||
| N | 10x | 10x | 100x | 100x | NTC | NTC | 10x | 10x | 100x | 100x | NTC | NTC | 10x | 10x | 100x | 100x | NTC | NTC | 30 | 0.049999999999999996 | 5 | 0.3 | 299.99999999999994 | ||||||||||||
| O | 10x | 10x | 100x | 100x | 10 | 10 | 100 | 100 | 10x | 10x | 100x | 100x | 10 | 10 | 100 | 100 | 10x | 10x | 100x | 100x | 10 | 10 | 100 | 100 | Sonda | ||||||||||
| P | 10x | 10x | 100x | 100x | 1000 | 1000 | 10k | 10k | 10x | 10x | 100x | 100x | 1000 | 1000 | 10k | 10k | 10x | 10x | 100x | 100x | 1000 | 1000 | 10k | 10k | 100 uM | 10 uM | 10+90 | 10 | 0.125 | 5 | 0.25 | 250 | |||
| 10 | 0.075 | 5 | 0.15 | 150 | |||||||||||||||||||||||||||||||
| RUN ON ROCHE LC480 MACHINE | |||||||||||||||||||||||||||||||||||
| amplikon | 18S (VIC!) | ||||||||||||||||||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | ||||||||||||
| A | 1000x | 1000x | 10kx | 10kx | 1000x | 1000x | 10kx | 10kx | |||||||||||||||||||||||||||
| B | 1000x | 1000x | 10kx | 10kx | 1000x | 1000x | 10kx | 10kx | |||||||||||||||||||||||||||
| C | 1000x | 1000x | 10kx | 10kx | 1000x | 1000x | 10kx | 10kx | |||||||||||||||||||||||||||
| D | 1000x | 1000x | 10kx | 10kx | 1000x | 1000x | 10kx | 10kx | |||||||||||||||||||||||||||
| E | 1000x | 1000x | 10kx | 10kx | 1000x | 1000x | 10kx | 10kx | |||||||||||||||||||||||||||
| F | 1000x | 1000x | 10kx | 10kx | 1000x | 1000x | 10kx | 10kx | |||||||||||||||||||||||||||
| G | 1000x | 1000x | 10kx | 10kx | 1000x | 1000x | 10kx | 10kx | |||||||||||||||||||||||||||
| H | 1000x | 1000x | 10kx | 10kx | 1000x | 1000x | 10kx | 10kx | |||||||||||||||||||||||||||
| I | 1000x | 1000x | 10kx | 10kx | |||||||||||||||||||||||||||||||
| J | 1000x | 1000x | 10kx | 10kx | |||||||||||||||||||||||||||||||
| K | 1000x | 1000x | 10kx | 10kx | |||||||||||||||||||||||||||||||
| L | 1000x | 1000x | 10kx | 10kx | |||||||||||||||||||||||||||||||
| M | 1000x | 1000x | 10kx | 10kx | |||||||||||||||||||||||||||||||
| N | 1000x | 1000x | 10kx | 10kx | NTC | NTC | |||||||||||||||||||||||||||||
| O | 1000x | 1000x | 10kx | 10kx | 10 | 10 | 100 | 100 | |||||||||||||||||||||||||||
| P | 1000x | 1000x | 10kx | 10kx | 1000 | 1000 | 10k | 10k |
