reports/december2016_feeding_assay

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weighing_nov2016

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Weighing individual larvae grown in plastic cups. Larvae hached 25.-28.11. Freshly detached potato plants cv. Desiree were exchanged every day.
dsRNA treatments were done at 2nd larval stage on 7.12. in 24-well plates (bottom 250 ul 1% agar) on leaf disks (R=1cm; 3x 0.5ul conc 0.5 ug/ul) and 11.12. in 24-well plates (R=1cm; 3x 1ul conc 0.5 ug/ul).
								mean larval mass					mass se
2016-12-08T0:0:0+0000	dpt [days post 1st treatment]			1			dpt [days post 1st treatment]	H2O	egfp	alas	JHBP	mesh	H2O	egfp	alas	JHBP	mesh
	treatments with dsRNAs:						1	10.1875	8.375	9.1875	9.375	10	0.449247055267663	0.4170831252080733	0.4934466367636255	0.40697051490249264	0.695221787153807
Larva	H2O	egfp	alas	JHBP	mesh		2	21.125	15.9375	17.125	19.0625	19.125	0.8702250666733674	0.5661915900705932	1.1542493953503579	1.0664143581804089	1.5380588415272025
1	12	8	11	8	10		3	42.125	31.0625	30.6875	36.266666666666666	28	1.827737672643424	1.8381801462315928	2.2980403789605903	2.2156728885840424	3.4250210555220284
2	8	5	11	10	11		4	70.75	53.13333333333333	47.5625	58.2	36.666666666666664	3.00069436408753	3.3691410049315382	3.996841200665012	5.229221921544534	10.397649306988148
3	10	8	7	9	6		5	91.625	73.26666666666667	71.78571428571429	83.92857142857143	47.5	3.9389878141471826	5.454108688621963	4.205149343924611	7.501255781837636	11.5
4	12	7	12	8	9		6	134.8	108.4	109.38461538461539	132.08333333333334	83	4.494652907834472	7.623147706320597	6.150399675921078	8.116069295421322
5	9	7	7	9	7		7	177.4	165.92857142857142	158.69230769230768	159.08333333333334	119	4.543755526622695	8.526921997069815	6.08211397900837	8.970032881711752
6	12	10	9	11	13
7	8	7	8	10	7
8	12	9	9	6	10
9	9	8	10	10	7
10	11	8	9	12	14
11	8	10	6	12	8
12	10	9	8	8	12
13	10	10	9	11	14
14	14	9	12	9	12
15	9	7	12	8	13
16	9	12	7	9	7
average:	10.1875	8.375	9.1875	9.375	10
median:	10	8	9	9	10
SE:	0.449247055267663	0.4170831252080733	0.4934466367636255	0.40697051490249264	0.695221787153807
dead larvae:	0	0	0	0	0
t-test p-val		0.006026497753656734	0.14453442587055654	0.19028437425723221	0.8225913655059007	slightly biased egfp sample?

2016-12-09T0:0:0+0000	dpt [days post 1st treatment]			2
	treatments with dsRNAs:
Larva	H2O	egfp	alas	JHBP	mesh
1	25	18	12	12	16
2	24	16	16	14	23
3	20	17	17	17	29
4	18	19	25	17	28
5	20	16	20	19	10
6	25	14	15	18	12
7	15	15	19	28	17
8	19	19	13	19	26
9	28	17	18	23	24
10	18	15	26	24	18
11	24	13	9	16	14
12	19	19	20	18	16
13	20	11	14	23	25
14	18	16	21	24	22
15	21	16	13	15	14
16	24	14	16	18	12
average:	21.125	15.9375	17.125	19.0625	19.125			larval survival
median:	20	16	16.5	18	17.5		dpt [days post 1st treatment]	H2O	egfp	alas	JHBP	mesh
SE:	0.8702250666733674	0.5661915900705932	1.1542493953503579	1.0664143581804089	1.5380588415272025		1	1	1	1	1	1
dead larvae:	0	0	0	0	0		2	1	1	1	1	1
t-test p-val		3.47071152286333E-5	0.009926736661627193	0.14488262771598037	0.2690569215992069		3	1	1	1	0.9375	0.8125
							4	1	0.9375	1	0.9375	0.1875
2016-12-10T0:0:0+0000	dpt [days post 1st treatment]			3			5	1	0.9375	0.875	0.875	0.125
	treatments with dsRNAs:						6	0.9375	0.9375	0.8125	0.75	0.0625
Larva	H2O	egfp	alas	JHBP	mesh		7	0.9375	0.875	0.8125	0.75	0.0625
1	39	28	36	25	18
2	55	35	31	37	35
3	50	23	30	28	48
4	35	34	21	33	22
5	39	41	30	45	45
6	36	28	27	38	22
7	38	32	43	39	31
8	35	37	17	26	8
9	53	29	27	55	44
10	49	33	16	48	15
11	35	17	39	27	27
12	53	34	39	32	19
13	44	37	45	39	30
14	38	30	25	40	dead
15	37	42	42	32	dead
16	38	17	23	dead	dead
average:	42.125	31.0625	30.6875	36.266666666666666	28
median:	38.5	32.5	30	37	27
SE:	1.827737672643424	1.8381801462315928	2.2980403789605903	2.2156728885840424	3.4250210555220284
dead larvae:	0	0	0	0.0625	0.1875
t-test p-val		1.8216778818547295E-4	5.420324692394101E-4	0.05106220946938147	0.001798545491758251

2016-12-11T0:0:0+0000	dpt [days post 1st treatment]			4
	treatments with dsRNAs:
Larva	H2O	egfp	alas	JHBP	mesh
1	86	56	65	47	45
2	83	45	39	80	16
3	69	43	83	73	49
4	61	56	56	33	dead
5	66	74	45	93	dead
6	79	50	52	72	dead
7	57	41	36	78	dead
8	66	36	67	81	dead
9	58	51	44	56	dead
10	85	64	34	50	dead
11	53	70	53	62	dead
12	73	63	53	34	dead
13	80	29	42	45	dead
14	57	68	43	33	dead
15	91	51	35	36	dead
16	68	dead	14	dead	dead
average:	70.75	53.13333333333333	47.5625	58.2	36.666666666666664
median:	68.5	51	44.5	56	45
SE:	3.00069436408753	3.3691410049315382	3.996841200665012	5.229221921544534	10.397649306988148
dead larvae:	0	0.0625	0	0.0625	0.8125
t-test p-val		5.331520368510841E-4	7.523599635798248E-5	0.048972800601822165	0.07138284863052094

2016-12-12T0:0:0+0000	dpt [days post 1st treatment]			5
	treatments with dsRNAs:
Larva	H2O	egfp	alas	JHBP	mesh
1	77	81	99	112	59
2	80	91	77	84	36
3	107	96	75	128	dead
4	91	68	91	70	dead
5	90	77	84	72	dead
6	73	62	62	97	dead
7	74	93	81	107	dead
8	75	71	71	81	dead
9	95	80	78	63	dead
10	117	66	53	86	dead
11	84	71	46	124	dead
12	80	95	68	76	dead
13	89	32	74	42	dead
14	111	90	46	33	dead
15	121	26	dead	dead	dead
16	102	dead	dead	dead	dead
average:	91.625	73.26666666666667	71.78571428571429	83.92857142857143	47.5
median:	89.5	77	74.5	82.5	47.5
SE:	3.9389878141471826	5.454108688621963	4.205149343924611	7.501255781837636	11.5
dead larvae:	0	0.0625	0.125	0.125	0.875
t-test p-val		0.011279982723914176	0.00185851279048324	0.3745679031922172	0.13108447699824213

2016-12-13T0:0:0+0000	dpt [days post 1st treatment]			6
	treatments with dsRNAs:
Larva	H2O	egfp	alas	JHBP	mesh
1	148	130	98	121	83
2	122	119	91	144	dead
3	127	98	138	110	dead
4	130	129	127	146	dead
5	104	127	102	177	dead
6	162	106	100	131	dead
7	151	97	158	123	dead
8	143	111	113	135	dead
9	135	131	92	186	dead
10	138	109	100	90	dead
11	117	107	73	105	dead
12	161	145	117	117	dead
13	148	129	113	dead	dead
14	123	48	dead	dead	dead
15	113	40	dead	dead	dead
16	dead	dead	dead	dead	dead
average:	134.8	108.4	109.38461538461539	132.08333333333334	83
median:	135	111	102	127	83
SE:	4.494652907834472	7.623147706320597	6.150399675921078	8.116069295421322
dead larvae:	0.0625	0.0625	0.1875	0.25	0.9375
t-test p-val		0.006710759745985407	0.0029044442466199393	0.7731037463207053

2016-12-14T0:0:0+0000	dpt [days post 1st treatment]			7
	treatments with dsRNAs:
Larva	H2O	egfp	alas	JHBP	mesh	SAMPLING DAY
1	199	178	147	203	119
2	153	80	167	157	dead
3	153	200	159	135	dead
4	192	191	152	142	dead
5	177	168	147	85	dead
6	203	187	125	188	dead
7	158	178	137	170	dead
8	166	182	205	175	dead
9	190	183	159	157	dead
10	191	145	172	141	dead
11	201	122	193	184	dead
12	171	164	142	172	dead
13	164	185	158	dead	dead
14	164	160	dead	dead	dead
15	179	dead	dead	dead	dead
16	dead	dead	dead	dead	dead
average:	177.4	165.92857142857142	158.69230769230768	159.08333333333334	119
median:	177	178	158	163.5	119
SE:	4.543755526622695	8.526921997069815	6.08211397900837	8.970032881711752
dead larvae:	0.0625	0.125	0.1875	0.25	0.9375
t-test p-val		0.2490729219050398	0.021637134694693423	0.0866772602056823