reports/october2016_feeding_assay

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weighing_nov2016

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Weighing individual larvae grown in plastic cups. Larvae were all hached on 16.10. Fresh potato leaves cv. Desiree were exchanged every day.
dsRNA treatments were done at 2nd larval stage on 20.10. in 96-well plates (bottom 20 ul 1% agar) on small leaf disks (2x 1ul of conc 0.5 ug/ul) and on 24.10. in 24-well plates (bottom 250 ul 1% agar) on leaf disks (R=1cm; 3x 1ul conc 0.5 ug/ul).
							mean larval mass				mass se
2016-12-08T0:0:0+0000	days old:	4				larval age [days]	H2O	egfp	alas	JHBP	H2O	egfp	alas	JHBP
	treatments with dsRNAs:					4	6.6875	6.875	5.875	4.75	0.45386075324193137	0.4460474563690878	0.40697051490249264	0.4232808366400098
Larva	H2O	egfp	alas	JHBP		5	11.5	12	11.375	9	0.8010409893798611	0.8106434833777776	0.7063698275926947	0.9692233691951199
1	8	8	5	4	due to a mistake in transferring larvae from the well plate I had to discard 3 larvae in the JHBP group and 6 larvae in the H2O group (the one from the H2O group were then replaced with the ones from the remaining colony)	6	18.25	17.8	16.125	14.818181818181818	1.1198958284888227	1.3172843638478722	1.33190027154188	2.048583465440457
2	7	7	4	6		7	28.375	27.8	23.1875	23	1.6903525273346582	2.46923932763571	1.6788357821220434	2.4312776705080625
3	6	10	7	7		8	39.46666666666667	33.09090909090909	30.714285714285715	30.375	2.184069305255383	2.2500688694694135	2.278491334842276	3.89568213355688
4	9	7	7	3		9	58.92857142857143	44.45454545454545	43.61538461538461	37	5.80589344925594	6.2338717523537	5.4837036678835736	6.519202405202648
5	5	6	9	4		10	87.61538461538461	61	52.54545454545455	50.42857142857143	7.283224896398486	10.317191693694774	7.9901799232940665	10.471860577069302
6	9	4	4	4		11	99.66666666666667	75.75	69.44444444444444	72.66666666666667	6.877903427321116	11.714485335116803	5.7084516073175555	16.841747072214464
7	9	8	8	5		12	142.5	93.5	93.42857142857143	105.6	5.133920807447545	9.29605651158956	4.965869908241471	11.483030958766934
8	6	9	6	4
9	6	8	4	4
10	8	9	7	6
11	5	4	6	3
12	7	7	7	3
13	4	5	6	9
14	4	5	6	5
15	5	6	3	3
16	9	7	5	6
average:	6.6875	6.875	5.875	4.75
median:	6.5	7	6	4
SE:	0.45386075324193137	0.4460474563690878	0.40697051490249264	0.4232808366400098
dead larvae:	0	0	0	0
t-test p-val		0.770293685153874	0.19273740792097194	0.00396950693353403	oops, somewhat biased sample for JHBP!!

2016-10-21T0:0:0+0000	days old:	5
	treatments with dsRNAs:
Larva	H2O	egfp	alas	JHBP	larval molting
1	15	13	13	9	removed due to accidental mixup
2	7	18	9	7
3	9	12	11	16
4	11	12	12	10
5	14	12	14	3
6	9	11	9	11
7	14	7	9	11
8	18	10	14	9
9	15	7	12	7
10	8	8	13	6
11	13	12	11	12
12	13	13	12	7
13	9	14	7	dead
14	8	16	13	removed
15	12	15	17	removed
16	9	dead	6	removed
average:	11.5	12	11.375	9
median:	11.5	12	12	9
SE:	0.8010409893798611	0.8106434833777776	0.7063698275926947	0.9692233691951199
dead larvae:	0	0.0625	0	0.07692307692307693
t-test p-val		0.6641155423784701	0.9076196590255805	0.05870208816697403

2016-10-22T0:0:0+0000	days old:	6
	treatments with dsRNAs:
Larva	H2O	egfp	alas	JHBP
1	24	26	27	22
2	25	15	20	16
3	14	17	22	16
4	26	21	20	14
5	16	27	19	11
6	12	9	19	31
7	16	16	13	13
8	23	14	18	6
9	20	15	15	14
10	19	25	19	11
11	19	16	15	9
12	15	12	13	dead
13	15	19	11	dead
14	16	18	12	removed
15	12	17	8	removed
16	20	dead	7	removed
average:	18.25	17.8	16.125	14.818181818181818
median:	17.5	17	16.5	14
SE:	1.1198958284888227	1.3172843638478722	1.33190027154188	2.048583465440457
dead larvae:	0	0.0625	0	0.15384615384615385
t-test p-val		0.7965664049255405	0.2318141644094615	0.16106858771217675

2016-10-23T0:0:0+0000	days old:	7
	treatments with dsRNAs:
Larva	H2O	egfp	alas	JHBP
1	27	20	36	23
2	26	26	22	18
3	33	19	32	16
4	38	38	24	25
5	33	47	16	20
6	24	12	25	28
7	29	35	19	10
8	16	26	14	32
9	38	30	26	22
10	27	26	26	36
11	36	30	31	dead
12	24	43	16	dead
13	37	22	21	dead
14	22	23	29	removed
15	21	20	13	removed
16	23	dead	21	removed
average:	28.375	27.8	23.1875	23
median:	27	26	23	22.5
SE:	1.6903525273346582	2.46923932763571	1.6788357821220434	2.4312776705080625
dead larvae:	0	0.0625	0	0.23076923076923078
t-test p-val		0.84917044169385	0.03744570608867845	0.08680419889675085

2016-10-24T0:0:0+0000	days old:	8
	treatments with dsRNAs:
Larva	H2O	egfp	alas	JHBP
1	42	32	45	44
2	40	47	20	35
3	38	26	26	44
4	30	32	38	30
5	46	39	36	21
6	36	35	25	12
7	31	35	21	25
8	36	33	39	32
9	38	17	25	dead
10	58	32	22	dead
11	50	36	31	dead
12	46	did not eat	30	dead
13	42	did not eat	45	dead
14	35	dead	27	removed
15	24	dead	did not eat	removed
16	dead	dead	did not eat	removed
average:	39.46666666666667	33.09090909090909	30.714285714285715	30.375
median:	38	33	28.5	31
SE:	2.184069305255383	2.2500688694694135	2.278491334842276	3.89568213355688
dead larvae:	0.0625	0.1875	0	0.38461538461538464
t-test p-val		0.053681830117184	0.009974655293389959	0.0654434428365054

2016-10-25T0:0:0+0000	days old:	9
	treatments with dsRNAs:
Larva	H2O	egfp	alas	JHBP
1	67	94	91	27
2	51	52	29	31
3	88	55	52	38
4	36	28	49	79
5	46	24	36	38
6	48	32	73	16
7	89	45	26	31
8	72	31	40	36
9	88	26	28	dead
10	74	41	52	dead
11	45	61	25	dead
12	63	did not eat	34	dead
13	39	did not eat	32	dead
14	19	dead	dead	removed
15	dead	dead	did not eat	removed
16	dead	dead	did not eat	removed
average:	58.92857142857143	44.45454545454545	43.61538461538461	37
median:	57	41	36	33.5
SE:	5.80589344925594	6.2338717523537	5.4837036678835736	6.519202405202648
dead larvae:	0.125	0.1875	0.0625	0.38461538461538464
t-test p-val		0.10334825534914541	0.06667775538817729	0.0225212208600719

2016-10-26T0:0:0+0000	days old:	10
	treatments with dsRNAs:
Larva	H2O	egfp	alas	JHBP
1	96	57	55	14	SAMPLED
2	114	78	38	35
3	89	24	22	43
4	60	81	29	44
5	101	50	32	104
6	65	74	50	57
7	61	29	59	56
8	116	121	52	dead
9	116	35	121	dead
10	126	dead	65	dead
11	87	dead	55	dead
12	54	did not eat	dead	dead
13	54	did not eat	dead	dead
14	dead	dead	dead	removed
15	dead	dead	did not eat	removed
16	dead	dead	did not eat	removed
average:	87.61538461538461	61	52.54545454545455	50.42857142857143
median:	89	57	52	44
SE:	7.283224896398486	10.317191693694774	7.9901799232940665	10.471860577069302
dead larvae:	0.1875	0.3125	0.1875	0.46153846153846156
t-test p-val		0.051840209911202285	0.0038406414484431807	0.013128916996876605

2016-10-27T0:0:0+0000	days old:	11
	treatments with dsRNAs:
Larva	H2O	egfp	alas	JHBP
1	130	79	76	61
2	92	102	78	52
3	79	45	86	93
4	115	77	72	13
5	86	dissapeared	50	82
6	85	dissapeared	94	135
7	74	dissapeared	55	dissapeared
8	114	dead	42	dissapeared
9	122	dead	72	dead
10	dissapeared	SAMPLED	dissapeared	dead
11	SAMPLED	SAMPLED	dissapeared	dead
12	SAMPLED	did not eat	dissapeared	dead
13	SAMPLED	did not eat	SAMPLED	dead
14	SAMPLED	dead	dead	removed
15	dead	dead	did not eat	removed
16	dead	dead	did not eat	removed
average:	99.66666666666667	75.75	69.44444444444444	72.66666666666667
median:	92	78	72	71.5
SE:	6.877903427321116	11.714485335116803	5.7084516073175555	16.841747072214464