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reference gene Referenčni geni
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| Data | Standard curve | ||||||||||||||||||||||||
| Involved | Sample name | Cq value | Average Cq value | Replicates Δ Cq | Relative quantity | log10(Relative quantity) | Slope | Slope difference | Calculated copy no. | CV copy no. | Average copy no. | dilution corrected copy no. | CV dilutions | Quality controled copy no. | Involved | Cq value | Relative quantity | Cq | Replicates Δ Cq | Relative quantity | log10(Relative quantity) | Parcial slope | |||
| no | HT115::dsGFP | 11.54 | / | / | 10000 | 4 | -3.18000000000002 | 0.03228571428573 | / | / | / | / | 1.47330367634989 | / | yes | 11.57 | 10000 | 11.57 | 0.13 | 10000 | 4 | -3.17500000000001 | |||
| no | HT115::dsGFP | 11.35 | 10000 | 4 | / | / | yes | 11.7 | 10000 | 11.7 | 10000 | 4 | |||||||||||||
| yes | HT115::dsGFP | 14.03 | 14.005 | 0.05 | 1000 | 3 | 1769.465299075639 | 2.58290002039474 | 1802.383786370901 | 1802.383786370901 | 1802.383786370901 | yes | 14.68 | 1000 | 14.68 | 0.26 | 1000 | 3 | -3.31000000000001 | ||||||
| yes | HT115::dsGFP | 13.98 | 1000 | 3 | 1835.302273666163 | 2.58290002039474 | yes | 14.94 | 1000 | 14.94 | 1000 | 3 | |||||||||||||
| yes | HT115::dsGFP | 17.21 | 17.185 | 0.05 | 100 | 2 | 173.2977445269528 | 2.58290002039489 | 176.5217125835676 | 1765.217125835676 | 1765.217125835676 | yes | 18.2 | 100 | 18.2 | 0.16 | 100 | 2 | -2.81 | ||||||
| yes | HT115::dsGFP | 17.16 | 100 | 2 | 179.7456806401825 | 2.58290002039489 | yes | 18.04 | 100 | 18.04 | 100 | 2 | |||||||||||||
| yes | HT115::dsMesh | 11.35 | 11.38 | 0.06 | 10000 | 4 | -3.1625 | 0.01478571428572 | 12538.21361614037 | 3.09932838616303 | 12269.32448463975 | 1226.932448463975 | 10.07663702627242 | 1226.932448463975 | yes | 20.92 | 10 | 20.92 | 0.02 | 10 | 1 | -3.44000000000001 | |||
| yes | HT115::dsMesh | 11.41 | 10000 | 4 | 12000.43535313914 | 3.09932838616303 | yes | 20.94 | 10 | 20.94 | 10 | 1 | |||||||||||||
| yes | HT115::dsMesh | 14.35 | 14.31 | 0.08 | 1000 | 3 | 1400.562564814874 | 4.13192330409009 | 1442.714513269711 | 1442.714513269711 | 1442.714513269711 | yes | 24.37 | 1 | 24.37 | 0 | 1 | 0 | / | ||||||
| yes | HT115::dsMesh | 14.27 | 1000 | 3 | 1484.866461724547 | 4.13192330409009 | no | 25.3 | 1 | ||||||||||||||||
| yes | HT115::dsMesh | 17.64 | 17.705 | 0.13 | 100 | 2 | 126.5753563689587 | 6.71124209000836 | 120.8407789105648 | 1208.407789105647 | 1208.407789105647 | ||||||||||||||
| yes | HT115::dsMesh | 17.77 | 100 | 2 | 115.1062014521708 | 6.71124209000836 | |||||||||||||||||||
| yes | dsMesh in-vitro | 33.91 | 33.43 | 0.95999999999999 | 10000 | 4 | -1.99000000000007 | 1.15771428571422 | 8.7035889395E-4 | 47.65910231791122 | 0.00131275998657 | 1.3127599866E-4 | 53.18808419323068 | / | Slope: | -3.14771428571428 | |||||||||
| yes | dsMesh in-vitro | 32.95 | 10000 | 4 | 0.00175516107918 | 47.65910231791122 | Inverse slope: | -0.317311050227 | |||||||||||||||||
| yes | dsMesh in-vitro | 35.32 | 35.42 | 0.2 | 1000 | 3 | 3.1066318525E-4 | 10.31440186642039 | 2.8954552006E-4 | 2.8954552006E-4 | / | Correlation cofficient (rsq): | 0.99880452581452 | ||||||||||||
| yes | dsMesh in-vitro | 35.52 | 1000 | 3 | 2.6842785486E-4 | 10.31440186642039 | Intercept: | 7.69971608480735 | |||||||||||||||||
| no | dsMesh in-vitro | Undetermined | / | / | 100 | 2 | / | / | / | / | / | ||||||||||||||
| no | dsMesh in-vitro | Undetermined | 100 | 2 | / | / | |||||||||||||||||||
| Experiment | |||||||||||||||||||||||||
| Name: | Ecoli_dsRNA_production | ||||||||||||||||||||||||
| Gene | |||||||||||||||||||||||||
| Name: | Referenčni geni 16S | ||||||||||||||||||||||||
| Type: | reference_gene | ||||||||||||||||||||||||
| Conditions | |||||||||||||||||||||||||
| Extraction quality control (CtExtC): | 25 | ||||||||||||||||||||||||
| Slope between: | between(-3.8, -3) | ||||||||||||||||||||||||
| Slope difference: | 0.5 |
