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AgroRNA_dsRNA_ciscena05-05-2016
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| NANODROP MEASUREMENTS | PREPARATION OF dsRNA LEAF SPRAY | |||||||||||||
| Sample ID | ng/ul | 260/280 | 260/230 | orig. conc. [ug/ul] | amount in 149 ml [ug] | larvae/leaf | ul for 10 ug/larva | 2x spray V for 1 leaf [ul] | n leaves | dsrna for n leaves [ul] | dH2O for n leaves [ul] | conc in spray [ug/ul] | tot spray V [ml] | |
| 1) | egfp dsRNA 50x | 100.11 | 2.39 | 2.63 | 5.0055 | 745.8195 | 10 | 19.978024173409253 | 168 | 30 | 599.3407252022776 | 5040 | 0.5319770778511336 | 5.639340725202277 |
| 2) | vATPaseE dsRNA 50x | 105.96 | 2.14 | 2.59 | 5.298 | 789.402 | 10 | 18.87504718761797 | 244 | 30 | 566.2514156285391 | 7320 | 0.38040887132443757 | 7.886251415628538 |
| 3) | alas dsRNA 50x | 119.62 | 2.12 | 2.51 | 5.981 | 891.169 | 10 | 16.719612104999165 | 264 | 30 | 501.58836314997495 | 7920 | 0.3562273374850505 | 8.421588363149974 |
| 4) | JHBP-like dsRNA 50x | 117.88 | 2.15 | 2.54 | 5.894 | 878.206 | 10 | 16.9664065151001 | 264 | 30 | 508.99219545300303 | 7920 | 0.35591443560931774 | 8.428992195453004 |
| 5) | mesh dsRNA 50x | 112.33 | 2.11 | 2.57 | 5.6165 | 836.8585 | 10 | 17.804682631532092 | 244 | 30 | 534.1404789459627 | 7320 | 0.38196413828373543 | 7.854140478945963 |
