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| First Author | Year | ug dsRNA used/larva | larvae/treatment group in one replicate | Treated instar | Feeding with treated leaves | Controls | Sampling RNA | Exp. Repetitions used for RNA isolation | Weigthing | Other measurements |
| Baum | 2007 | varying | 40 | neonate | NOT LEAVES - ARTIFICIAL DIET! | untreated | 12h, 1d, 2d, 4d, 6d | 1 | 0-12 dpt | larval stunting, mortality |
| Zhu | 2011 | 10 | 20 | 2nd | starved 6h prior, 5 larvae/petri dish, fed on treated leaves 6 days, 6 on nontreated | EV, malE | 6 dpt, 3 larvae/treatment | 3 | 0, 3, 6 , 9 dpt | survival 3, 6, 9, 12 dpt |
| Bachman | 2013 | 2.5 | 24 | neonate | NOT LEAVES - ARTIFICIAL DIET! | untreated | NA | 1 | NA | survival 12 dpt |
| Zhou | 2013 | leaf dipped in dsRNA | 10 | eggs, 1st, 2nd, 3rd, 4th, pupa, adult | starved 4 h prior, 3 days, then transffered to untreated leaves | PBS buffer, egfp | 3 dpt and 5 dpt, 10 larvae/treatment | 3 | 0, 3, 6, 9 dpt | survival 3, 6, 9 dpt; pupation; adult emergence in 4 week period |
| Fu | 2014 | leaf dipped in dsRNA | 10 | 2nd||4th | starved 2 h prior, 3 days, then transffered to untreated leaves in cup with soil, 10 larvae per petri dish | PBS buffer, egfp | 4 dpt and 6 dpt, 10 larvae/treatment | 3||3 | 3 dpt | growth/instars; pupation; adult emergence in 4 week period; adult weight at emergence; eggs per female; JH and 20E titer; expression of hormonal pathway genes |
| Liu | 2014 | leaf dipped in dsRNA | 10 | 4th | 3 days, then transffered to untreated leaves in cup with soil, 10 larvae per petri dish | PBS buffer, egfp | 3 dpt, 10 larvae/treatment | 3 | NA | pupation rate; pupal progress; JH and 20E titer; expression of hormonal pathway genes |
| Miguel and Scott | 2015 | 2.5 | 40-140 | 2nd | 4 days, then replaced with untreated leaves | tap water, egfp | 7 dpt, all surviving larve | 1 | 7 dpt | 7 dpt; % mortality, % reaching 4th instar |
| Shi | 2015 | leaf dipped in dsRNA | 10 | 2nd||4th | starved 4 h prior, 3 days, then transffered to untreated leaves, 10 larvae per petri dish | PBS buffer, egfp | 3 dpt, 10 larvae/treatment | 3||3 | 3 dpt | growth/instars; pupation; adult emergence in 4 week period; JH and 20E titer |
| Meng | 2015 | leaf dipped in dsRNA | 10 | 2nd | starved 2 h prior,10 larvae per petri dish, replaced leaf every day | PBS buffer, egfp | 3 dpt and 6 dpt, 10 larvae/treatment | 3 | 4 dpt | growth/instars; pupation; adult emergence in 4 week period; JH titer; expression of hormonal pathway genes |
| Zhu | 2015 | leaf dipped in dsRNA | 10 | 3rd||4th | starved 4 h prior, 3 days, then transffered to untreated leaves | PBS buffer, egfp | 3 dpt, 10 larvae/treatment | 3||3 | 3 dpt | 4th instar rate; pupation rate; adult emergence in 4 week period; pupal weight; JH and 20E titer; expression of hormonal pathway genes |
| Lü | 2015 | leaf dipped in dsRNA | 10 | 4th | 3 days, then transffered to untreated leaves in cup with soil, 10 larvae per petri dish | PBS buffer, egfp | 3 dpt, 10 larvae/treatment | 3 | 3dpt? | survivorship; pupation rate; pupal progress; JH and 20E titer; expression of hormonal pathway genes |
| Fu | 2016 | leaf dipped in dsRNA | 21 | 3rd newly-ecdysed | 3 days, then transffered to plastic cups with untreated leaves | PBS buffer, egfp | 3 dpt, 9 larvae/treatment | 1 | 3 dpt | remaining 12 left for observation - pupation checked for 3 larvae on day 4,8, 12 after pupation, 3 left for adult emergence |
| Kong | 2016 | leaf dipped in dsRNA | 10 | 2nd||4th | starved 4 h prior, 10 larvae per petri dish, replaced leaf every day | PBS buffer, egfp | 4 dpt and 5 dpt, 10 larvae/treatment | 3||3 | 5, 9 dpt | survival 5, 9 dpt; pupation; adult emergence in 4 week period |
| Guo | 2015a | varying | 10 | 1st, 2nd, 3rd, 4th||4th | 6 days, then transffered to untreated leaves in cup with soil, 10 larvae per petri dish | PBS buffer, egfp | 3 dpt, 10 larvae/treatment||3 dpt, 10 larvae/treatment | 3 | 6 dpt | mortality 6 dpt, pupation during 4 weeks, RNAi pathway gene expression |
| Guo | 2015b | leaf dipped in dsRNA | 10 | 4th | 3 days, then transffered to untreated leaves in cup with soil for another 5 days, 10 larvae per petri dish | PBS buffer, egfp | 3 dpt, 10 larvae/treatment | 3 | NA | survivorship; pupation rate; 20E titer; expression of hormonal pathway genes |
