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| qPCR for testing efficiency of knockdown with dsRNAs | |||||||||||||
| treatments: | water | dsegfp | dsvATPaseE | dsalas | dsJHBP | dsmesh | SUM | ||||||
| jun2016_target_selection_5dpt | 4 | 4 | 4 | 4 | 4 | 4 | 24 | ||||||
| jun2016_mesh_RNAseq_1dpt | 4 | 3 | 7 | ||||||||||
| jul2016_MeJA_14dpt | 8 | 8 | 8 | 24 | |||||||||
| oct2016_2x1ug_8dpt | 4 | 4 | 3 | 11 | |||||||||
| dec2016_2x1ug_7dpt | 4 | 4 | 4 | 4 | 1 | 17 | |||||||
| SUM | 12 | 24 | 4 | 19 | 16 | 8 | 83 | ||||||
| na kontrolah qPCR za vse gene v poskusu | |||||||||||||
| water | dsegfp | dsvATPaseE | dsalas | dsJHBP | dsmesh | SUM | |||||||
| jun2016_target_selection_5dpt | 4 | 4 | 4 | 4 | 4 | 4 | 24 | ||||||
| dec2016_2x1ug_7dpt | 4 | 4 | 4 | 4 | 16 | ||||||||
| SUM | 8 | 8 | 4 | 8 | 8 | 4 | 40 | ||||||
| vsi vzorci na 18S in RP4 - na robota | 1 plata, 2 amplikona, 40 vzorcev! | ||||||||||||
| 16 kontrolnih vzorcev na vseh 4 tarčnih amplikonih + | |||||||||||||
| -- alas in JHBP dodatno 8 vz | mix na robota za 24 vz,12 vzorcev na robota, 8 za vsak amplikon na roke? | 1 plata, 2 amplikona, 24 vz. | |||||||||||
| -- vATPaseE in mesh dodatno 4 vz | mix na robota za 20 vz, 12 vzorcev na robota, 4 za vsak amplikon na roke? | 1 plata, 2 amplikona, 20 vz. | |||||||||||
