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{"sheet":"markop27-03-2018","index":0,"n_rows":120,"n_cols":33,"rows_kept":120,"cut":false,"hidden":false,"merged":[]}
["SDS 2.4","AQ Results",1]
["Filename","markop27-03-2018"]
["PlateID"]
["Assay Type","Absolute Quantification"]
["Run DateTime","3/27/18 6:18:27 PM"]
["Operator"]
["ThermalCycleParams"]
[]
["Sample Information"]
[]
["Well","Sample Name","Detector Name","Reporter","Task","Ct","cDNA","dCt","Quantity","Qty Mean","Qty StdDev","Ct Median","Ct Mean","Ct StdDev","Ct Type","Template Name","Baseline Type","Baseline Start","Baseline Stop","Threshold Type","Threshold","FOS","HMD","LME","EW","BPR","NAW","HNS","HRN","EAF","BAF","TAF","CAF"]
[97,"E1","dsGFP","SYBR","HT115::dsGFP",20.865969,10000,{"$f":"AVERAGE(F14:F15)-AVERAGE(F12:F13)","v":3.2825935000000044},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.18199947]
[98,"E2","dsGFP","SYBR","HT115::dsGFP",20.524393,10000,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.18199947]
[99,"E3","dsGFP","SYBR","HT115::dsGFP",24.130405,1000,{"$f":"AVERAGE(F16:F17)-AVERAGE(F14:F15)","v":3.6492949999999986},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.18199947]
[100,"E4","dsGFP","SYBR","HT115::dsGFP",23.825144000000002,1000,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.18199947]
[101,"E5","dsGFP","SYBR","HT115::dsGFP",27.419460000000001,100,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.18199947]
[102,"E6","dsGFP","SYBR","HT115::dsGFP",27.834679000000001,100,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.18199947]
[null,null,"dsGFP","SYBR","HT115::dsMesh",null,10000]
[null,null,"dsGFP","SYBR","HT115::dsMesh",null,10000]
[null,null,"dsGFP","SYBR","HT115::dsMesh",null,1000]
[null,null,"dsGFP","SYBR","HT115::dsMesh",null,1000]
[null,null,"dsGFP","SYBR","HT115::dsMesh",null,100]
[null,null,"dsGFP","SYBR","HT115::dsMesh",null,100]
[null,null,"dsGFP","SYBR","dsMesh in-vitro",null,10000]
[null,null,"dsGFP","SYBR","dsMesh in-vitro",null,10000]
[null,null,"dsGFP","SYBR","dsMesh in-vitro",null,1000]
[null,null,"dsGFP","SYBR","dsMesh in-vitro",null,1000]
[null,null,"dsGFP","SYBR","dsMesh in-vitro",null,100]
[null,null,"dsGFP","SYBR","dsMesh in-vitro",null,100]
[115,"E19","dsGFP","SYBR","Unknown",20.722065000000001,10000,{"$f":"AVERAGE(F32:F33)-AVERAGE(F30:F31)","v":3.6300264999999996},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.18199947]
[116,"E20","dsGFP","SYBR","Unknown",21.533809999999999,10000,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.18199947]
[117,"E21","dsGFP","SYBR","Unknown",24.679556000000002,1000,{"$f":"AVERAGE(F34:F35)-AVERAGE(F32:F33)","v":3.9321029999999979},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.18199947]
[118,"E22","dsGFP","SYBR","Unknown",24.836372000000001,1000,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.18199947]
[119,"E23","dsGFP","SYBR","Unknown",28.631209999999999,100,{"$f":"AVERAGE(F36:F37)-AVERAGE(F34:F35)","v":4.1803060000000016},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.18199947]
[120,"E24","dsGFP","SYBR","Unknown",28.748923999999999,100,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.18199947]
[139,"F19","dsGFP","SYBR","Unknown",32.380108,10,{"$f":"AVERAGE(F38:F39)-AVERAGE(F36:F37)","v":3.0184984999999998},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.18199947]
[140,"F20","dsGFP","SYBR","Unknown",33.360638000000002,10,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.18199947]
[141,"F21","dsGFP","SYBR","Unknown",37.037193000000002,1,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.18199947]
[142,"F22","dsGFP","SYBR","Unknown",34.740549999999999,1,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.18199947]
[143,"F23","dsGFP","SYBR","Unknown","Undetermined","NTC",{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.18199947,{"$nulls":5},true,null,null,true]
[144,"F24","dsGFP","SYBR","Unknown","Undetermined","NTC",{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.18199947,{"$nulls":5},true,null,null,true]
["Slope",null,"cycles/log decade"]
["Y-Intercept"]
["R^2"]
[]
[]
["Well","Sample Name","Detector Name","Reporter","Task","Ct","cDNA","dCt","Quantity","Qty Mean","Qty StdDev","Ct Median","Ct Mean","Ct StdDev","Ct Type","Template Name","Baseline Type","Baseline Start","Baseline Stop","Threshold Type","Threshold","FOS","HMD","LME","EW","BPR","NAW","HNS","HRN","EAF","BAF","TAF","CAF"]
[null,null,"dsMesh","SYBR","HT115::dsGFP",null,10000]
[null,null,"dsMesh","SYBR","HT115::dsGFP",null,10000]
[null,null,"dsMesh","SYBR","HT115::dsGFP",null,1000]
[null,null,"dsMesh","SYBR","HT115::dsGFP",null,1000]
[null,null,"dsMesh","SYBR","HT115::dsGFP",null,100]
[null,null,"dsMesh","SYBR","HT115::dsGFP",null,100]
[49,"C1","dsMesh","SYBR","HT115::dsMesh",8.9545429999999993,10000,{"$f":"AVERAGE(F56:F57)-AVERAGE(F54:F55)","v":2.7279904999999989},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998]
[50,"C2","dsMesh","SYBR","HT115::dsMesh",9.9699170000000006,10000,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998]
[51,"C3","dsMesh","SYBR","HT115::dsMesh",12.077254999999999,1000,{"$f":"AVERAGE(F58:F59)-AVERAGE(F56:F57)","v":3.3240550000000013},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998]
[52,"C4","dsMesh","SYBR","HT115::dsMesh",12.303186,1000,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998]
[53,"C5","dsMesh","SYBR","HT115::dsMesh",15.377641000000001,100,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998]
[54,"C6","dsMesh","SYBR","HT115::dsMesh",15.65091,100,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998]
[55,"C7","dsMesh","SYBR","dsMesh in-vitro",14.807805,10000,{"$f":"AVERAGE(F62:F63)-AVERAGE(F60:F61)","v":3.1707214999999991},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998]
[56,"C8","dsMesh","SYBR","dsMesh in-vitro",14.734721,10000,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998]
[57,"C9","dsMesh","SYBR","dsMesh in-vitro",17.918835000000001,1000,{"$f":"AVERAGE(F64:F65)-AVERAGE(F62:F63)","v":4.2718264999999995},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998]
[58,"C10","dsMesh","SYBR","dsMesh in-vitro",17.965133999999999,1000,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998]
[59,"C11","dsMesh","SYBR","dsMesh in-vitro",22.035515,100,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998]
[60,"C12","dsMesh","SYBR","dsMesh in-vitro",22.392106999999999,100,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998]
[67,"C19","dsMesh","SYBR","Unknown",9.785736,10000,{"$f":"AVERAGE(F68:F69)-AVERAGE(F66:F67)","v":3.3896937500000011},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998]
[68,"C20","dsMesh","SYBR","Unknown",9.9397859999999998,10000,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998]
[69,"C21","dsMesh","SYBR","Unknown",13.217267,1000,{"$f":"AVERAGE(F70:F71)-AVERAGE(F68:F69)","v":3.62318125},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998]
[70,"C22","dsMesh","SYBR","Unknown",13.2876425,1000,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998]
[71,"C23","dsMesh","SYBR","Unknown",16.795953999999998,100,{"$f":"AVERAGE(F72:F73)-AVERAGE(F70:F71)","v":3.4398519999999984},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998]
[72,"C24","dsMesh","SYBR","Unknown",16.955317999999998,100,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998]
[91,"D19","dsMesh","SYBR","Unknown",20.290292999999998,10,{"$f":"AVERAGE(F74:F75)-AVERAGE(F72:F73)","v":3.6135405000000027},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998]
[92,"D20","dsMesh","SYBR","Unknown",20.340682999999999,10,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998]
[93,"D21","dsMesh","SYBR","Unknown",23.939769999999999,1,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998]
[94,"D22","dsMesh","SYBR","Unknown",23.918286999999999,1,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998]
[95,"D23","dsMesh","SYBR","Unknown","Undetermined","NTC",{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998,{"$nulls":5},true,null,null,true]
[96,"D24","dsMesh","SYBR","Unknown","Undetermined","NTC",{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",0.64408379999999998,{"$nulls":5},true,null,null,true]
["Slope",null,"cycles/log decade"]
["Y-Intercept"]
["R^2"]
[]
[]
["Well","Sample Name","Detector Name","Reporter","Task","Ct","cDNA","dCt","Quantity","Qty Mean","Qty StdDev","Ct Median","Ct Mean","Ct StdDev","Ct Type","Template Name","Baseline Type","Baseline Start","Baseline Stop","Threshold Type","Threshold","FOS","HMD","LME","EW","BPR","NAW","HNS","HRN","EAF","BAF","TAF","CAF"]
[1,"A1","E.coli_16SrDNA","FAM","HT115::dsGFP",11.544214,10000,{"$f":"AVERAGE(F86:F87)-AVERAGE(F84:F85)","v":2.5569884999999992},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true]
[2,"A2","E.coli_16SrDNA","FAM","HT115::dsGFP",11.354792,10000,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true]
[3,"A3","E.coli_16SrDNA","FAM","HT115::dsGFP",14.029467,1000,{"$f":"AVERAGE(F88:F89)-AVERAGE(F86:F87)","v":3.1763145000000002},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true]
[4,"A4","E.coli_16SrDNA","FAM","HT115::dsGFP",13.983516,1000,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true]
[5,"A5","E.coli_16SrDNA","FAM","HT115::dsGFP",17.209896000000001,100,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true]
[6,"A6","E.coli_16SrDNA","FAM","HT115::dsGFP",17.155716000000002,100,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true]
[7,"A7","E.coli_16SrDNA","FAM","HT115::dsMesh",11.349843,10000,{"$f":"AVERAGE(F92:F93)-AVERAGE(F90:F91)","v":2.9293929999999992},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true]
[8,"A8","E.coli_16SrDNA","FAM","HT115::dsMesh",11.413762999999999,10000,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true]
[9,"A9","E.coli_16SrDNA","FAM","HT115::dsMesh",14.348087,1000,{"$f":"AVERAGE(F94:F95)-AVERAGE(F92:F93)","v":3.3942720000000008},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true]
[10,"A10","E.coli_16SrDNA","FAM","HT115::dsMesh",14.274305,1000,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true]
[11,"A11","E.coli_16SrDNA","FAM","HT115::dsMesh",17.641565,100,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true]
[12,"A12","E.coli_16SrDNA","FAM","HT115::dsMesh",17.769371,100,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true]
[13,"A13","E.coli_16SrDNA","FAM","dsMesh in-vitro",33.914645999999998,10000,{"$f":"AVERAGE(F98:F99)-AVERAGE(F96:F97)","v":1.9869825000000034},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true]
[14,"A14","E.coli_16SrDNA","FAM","dsMesh in-vitro",32.945152,10000,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true]
[15,"A15","E.coli_16SrDNA","FAM","dsMesh in-vitro",35.317318,1000,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true]
[16,"A16","E.coli_16SrDNA","FAM","dsMesh in-vitro",35.516444999999997,1000,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true]
[17,"A17","E.coli_16SrDNA","FAM","dsMesh in-vitro","Undetermined",100,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true,null,null,true]
[18,"A18","E.coli_16SrDNA","FAM","dsMesh in-vitro","Undetermined",100,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true,null,null,true]
[19,"A19","E.coli_16SrDNA","FAM","Unknown",11.569440999999999,10000,{"$f":"AVERAGE(F104:F105)-AVERAGE(F102:F103)","v":3.1774830000000005},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true]
[20,"A20","E.coli_16SrDNA","FAM","Unknown",11.696725000000001,10000,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true]
[21,"A21","E.coli_16SrDNA","FAM","Unknown",14.679221,1000,{"$f":"AVERAGE(F106:F107)-AVERAGE(F104:F105)","v":3.3100194999999974},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true,null,null,true]
[22,"A22","E.coli_16SrDNA","FAM","Unknown",14.941910999999999,1000,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true,null,null,true]
[23,"A23","E.coli_16SrDNA","FAM","Unknown",18.203769999999999,100,{"$f":"AVERAGE(F108:F109)-AVERAGE(F106:F107)","v":2.8070060000000012},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true,null,null,true]
[24,"A24","E.coli_16SrDNA","FAM","Unknown",18.037400999999999,100,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true,null,null,true]
[43,"B19","E.coli_16SrDNA","FAM","Unknown",20.9191,10,{"$f":"AVERAGE(F110:F111)-AVERAGE(F108:F109)","v":3.9106665000000049},{"$nulls":6},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true,null,null,true]
[44,"B20","E.coli_16SrDNA","FAM","Unknown",20.936083,10,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true,null,null,true]
[45,"B21","E.coli_16SrDNA","FAM","Unknown",24.372696000000001,1,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true,null,null,true]
[46,"B22","E.coli_16SrDNA","FAM","Unknown",25.303820000000002,1,{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true,null,null,true]
[47,"B23","E.coli_16SrDNA","FAM","Unknown",36.606856999999998,"NTC",{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true,null,null,true]
[48,"B24","E.coli_16SrDNA","FAM","Unknown","Undetermined","NTC",{"$nulls":7},"Automatic Ct",null,"Automatic",null,null,"Automatic",2.8777357E-2,{"$nulls":5},true,null,null,true]
["Slope",null,"cycles/log decade"]
["Y-Intercept"]
["R^2"]
[]
[]
[]
["NAP","LPL"]