{"text_format":"2","workbook":"xlsx","n_sheets":4,"sheets":["NIB","LC480","LC480+EFF","RNAinVAL_Ecoli_ds_production"],"not_rendered":{"charts":0,"images":0,"macros":0,"external_links":0,"defined_names":1,"comments":0,"pivot_tables":0},"unopened":[],"bounds":{"document_bytes":16777216,"sheet_cells":100000,"run_cost":"one","rows_first":2000,"rows_last":500,"rows_between":2000,"halvings":0,"cell_chars":16384,"cell_head_chars":4096},"cells_cut":0,"cells_raw":0,"read":"whole","date_system":"1900"}

{"sheet":"NIB","index":0,"n_rows":38,"n_cols":26,"rows_kept":38,"cut":false,"hidden":true,"merged":[]}
[null,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24]
["A","0+1",null,null,null,"kont3-2",null,null,null,"bliz3wt3",null,null,null,"kont24+1",null,null,null,"bliz24-2",null,null,null,"inok24wt3",null,null,null," - 2 red?itvi -- za preverjanje u?inkovitosti vzorca"]
["B","0+2",null,null,null,"kont3-3",null,null,null,"bliz3coi1",null,null,null,"kont24+2",null,null,null,"bliz24-3",null,null,null,"inok24coi1",null,null,null,"   - ?e k preve? odstopa od k umeritvene, se vzorec izlo?i"]
["C","0+3",null,null,null,"kont3wt1",null,null,null,"bliz3coi2",null,null,null,"kont24+3",null,null,null,"bliz24wt1",null,null,null,"inok24coi2"]
["D","0-1",null,null,null,"kont3wt2",null,null,null,"bliz3coi3",null,null,null,"kont24-1",null,null,null,"bliz24wt2",null,null,null,"inok24coi3"]
["E","0-2",null,null,null,"kont3wt3",null,null,null,"inok3+1",null,null,null,"kont24-2",null,null,null,"bliz24wt3"]
["F","0-3",null,null,null,"kont3coi1",null,null,null,"inok3+2",null,null,null,"kont24-3",null,null,null,"bliz24coi1"]
["G","0wt1",null,null,null,"kont3coi2",null,null,null,"inok3+3",null,null,null,"kont24wt1",null,null,null,"bliz24coi2"]
["H","0wt2",null,null,null,"kont3coi3",null,null,null,"inok3-1",null,null,null,"kont24wt2",null,null,null,"bliz24coi3"]
["I","0wt3",null,null,null,"bliz3+1",null,null,null,"inok3-2",null,null,null,"kont24wt3",null,null,null,"inok24+1"]
["J","0coi1",null,null,null,"bliz3+2",null,null,null,"inok3-3",null,null,null,"kont24coi1",null,null,null,"inok24+2"]
["K","0coi2",null,null,null,"bliz3+3",null,null,null,"inok3wt1",null,null,null,"kont24coi2",null,null,null,"inok24+3"]
["L","0coi3",null,null,null,"bliz3-1",null,null,null,"inok3wt2",null,null,null,"kont24coi3",null,null,null,"inok24-1",{"$nulls":5},"ntc"]
["M","kont3+1",null,null,null,"bliz3-2",null,null,null,"inok3wt3",null,null,null,"bliz24+1",null,null,null,"inok24-2",{"$nulls":5},"10 x",null,"umeritvena krivulja kalibracijskega vzorca"]
["N","kont3+2",null,null,null,"bliz3-3",null,null,null,"inok3coi1",null,null,null,"bliz24+2",null,null,null,"inok24-3",{"$nulls":5},"100 x",null," -  glede na to ra?unamo abs. koli?ine cDNA"]
["O","kont3+3",null,null,null,"bliz3wt1",null,null,null,"inok3coi2",null,null,null,"bliz24+3",null,null,null,"inok24wt1",{"$nulls":5},"1000 x"]
["P","kont3-1",null,null,null,"bliz3wt2",null,null,null,"inok3coi3",null,null,null,"bliz24-1",null,null,null,"inok24wt2",{"$nulls":5},"10000 x"]
[]
["bolj logi?no PO SKUPINAH:"]
[null,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24]
["A","0+1",null,null,null,"bliz24+2",null,null,null,"inok3-3",null,null,null,"bliz3wt1",null,null,null,"0coi2",null,null,null,"bliz24coi3",null,null,null," - 2 red?itvi -- za preverjanje u?inkovitosti vzorca"]
["B","0+2",null,null,null,"bliz24+3",null,null,null,"kont24-1",null,null,null,"bliz3wt2",null,null,null,"0coi3",null,null,null,"inok24coi1",null,null,null,"   - ?e k preve? odstopa od k umeritvene, se vzorec izlo?i"]
["C","0+3",null,null,null,"inok24+1",null,null,null,"kont24-2",null,null,null,"bliz3wt3",null,null,null,"kont3coi1",null,null,null,"inok24coi2"]
["D","kont3+1",null,null,null,"inok24+2",null,null,null,"kont24-3",null,null,null,"inok3wt1",null,null,null,"kont3coi2",null,null,null,"inok24coi3"]
["E","kont3+2",null,null,null,"inok24+3",null,null,null,"bliz24-1",null,null,null,"inok3wt2",null,null,null,"kont3coi3"]
["F","kont3+3",null,null,null,"0-1",null,null,null,"bliz24-2",null,null,null,"inok3wt3",null,null,null,"bliz3coi1"]
["G","bliz3+1",null,null,null,"0-2",null,null,null,"bliz24-3",null,null,null,"kont24wt1",null,null,null,"bliz3coi2"]
["H","bliz3+2",null,null,null,"0-3",null,null,null,"inok24-1",null,null,null,"kont24wt2",null,null,null,"bliz3coi3"]
["I","bliz3+3",null,null,null,"kont3-1",null,null,null,"inok24-2",null,null,null,"kont24wt3",null,null,null,"inok3coi1"]
["J","inok3+1",null,null,null,"kont3-2",null,null,null,"inok24-3",null,null,null,"bliz24wt1",null,null,null,"inok3coi2"]
["K","inok3+2",null,null,null,"kont3-3",null,null,null,"0wt1",null,null,null,"bliz24wt2",null,null,null,"inok3coi3"]
["L","inok3+3",null,null,null,"bliz3-1",null,null,null,"0wt2",null,null,null,"bliz24wt3",null,null,null,"kont24coi1",{"$nulls":5},"ntc",null,"umeritvena krivulja kalibracijskega vzorca"]
["M","kont24+1",null,null,null,"bliz3-2",null,null,null,"0wt3",null,null,null,"inok24wt1",null,null,null,"kont24coi2",{"$nulls":5},"10 x",null," -  glede na to ra?unamo abs. koli?ine cDNA"]
["N","kont24+2",null,null,null,"bliz3-3",null,null,null,"kont3wt1",null,null,null,"inok24wt2",null,null,null,"kont24coi3",{"$nulls":5},"100 x"]
["O","kont24+3",null,null,null,"inok3-1",null,null,null,"kont3wt2",null,null,null,"inok24wt3",null,null,null,"bliz24coi1",{"$nulls":5},"1000 x"]
["P","bliz24+1",null,null,null,"inok3-2",null,null,null,"kont3wt3",null,null,null,"0coi1",null,null,null,"bliz24coi2",{"$nulls":5},"10000 x"]
[]
[null,"NA VSAKI PLOŠ?I?I BI MERIL SAMO 1 GEN - v enem run-u bi pomeril vse vzorce!!!"]

{"sheet":"LC480","index":1,"n_rows":54,"n_cols":26,"rows_kept":54,"cut":false,"hidden":true,"merged":[]}
[null,"References",{"$nulls":5},"Targets"]
[null,"18S",null,"COX",null,"EF-2",null,"ETR1",null,"ETR4",null,"CTR1",null,"EIN3",null,"ERF1",null,"WIPK",null,"JAR1",null,"coi1",null,"StPPS3"]
[null,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24]
["A","0+1",null,"0+1",null,"0+1",null,"0+1",null,"0+1",null,"0+1",null,"0+1",null,"0+1",null,"0+1",null,"0+1",null,"0+1",null,"0+1"]
["B","0+2",null,"0+2",null,"0+2",null,"0+2",null,"0+2",null,"0+2",null,"0+2",null,"0+2",null,"0+2",null,"0+2",null,"0+2",null,"0+2"]
["C","0+3",null,"0+3",null,"0+3",null,"0+3",null,"0+3",null,"0+3",null,"0+3",null,"0+3",null,"0+3",null,"0+3",null,"0+3",null,"0+3"]
["D","kont3+1",null,"kont3+1",null,"kont3+1",null,"kont3+1",null,"kont3+1",null,"kont3+1",null,"kont3+1",null,"kont3+1",null,"kont3+1",null,"kont3+1",null,"kont3+1",null,"kont3+1"]
["E","kont3+2",null,"kont3+2",null,"kont3+2",null,"kont3+2",null,"kont3+2",null,"kont3+2",null,"kont3+2",null,"kont3+2",null,"kont3+2",null,"kont3+2",null,"kont3+2",null,"kont3+2"]
["F","kont3+3",null,"kont3+3",null,"kont3+3",null,"kont3+3",null,"kont3+3",null,"kont3+3",null,"kont3+3",null,"kont3+3",null,"kont3+3",null,"kont3+3",null,"kont3+3",null,"kont3+3"]
["G","bliz3+1",null,"bliz3+1",null,"bliz3+1",null,"bliz3+1",null,"bliz3+1",null,"bliz3+1",null,"bliz3+1",null,"bliz3+1",null,"bliz3+1",null,"bliz3+1",null,"bliz3+1",null,"bliz3+1"]
["H","bliz3+2",null,"bliz3+2",null,"bliz3+2",null,"bliz3+2",null,"bliz3+2",null,"bliz3+2",null,"bliz3+2",null,"bliz3+2",null,"bliz3+2",null,"bliz3+2",null,"bliz3+2",null,"bliz3+2"]
["I","bliz3+3",null,"bliz3+3",null,"bliz3+3",null,"bliz3+3",null,"bliz3+3",null,"bliz3+3",null,"bliz3+3",null,"bliz3+3",null,"bliz3+3",null,"bliz3+3",null,"bliz3+3",null,"bliz3+3"]
["J","inok3+1",null,"inok3+1",null,"inok3+1",null,"inok3+1",null,"inok3+1",null,"inok3+1",null,"inok3+1",null,"inok3+1",null,"inok3+1",null,"inok3+1",null,"inok3+1",null,"inok3+1"]
["K","inok3+2",null,"inok3+2",null,"inok3+2",null,"inok3+2",null,"inok3+2",null,"inok3+2",null,"inok3+2",null,"inok3+2",null,"inok3+2",null,"inok3+2",null,"inok3+2",null,"inok3+2"]
["L","inok3+3",null,"inok3+3",null,"inok3+3",null,"inok3+3",null,"inok3+3",null,"inok3+3",null,"inok3+3",null,"inok3+3",null,"inok3+3",null,"inok3+3",null,"inok3+3",null,"inok3+3"]
["M","ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc"]
["N"]
["O",{"$f":"\"PosCal\"&\" \"&B2","v":"PosCal 18S"},null,{"$f":"\"PosCal\"&\" \"&D2","v":"PosCal COX"},null,{"$f":"\"PosCal\"&\" \"&F2","v":"PosCal EF-2"},null,{"$f":"\"PosCal\"&\" \"&H2","v":"PosCal ETR1"},null,{"$f":"\"PosCal\"&\" \"&J2","v":"PosCal ETR4"},null,{"$f":"\"PosCal\"&\" \"&L2","v":"PosCal CTR1"},null,{"$f":"\"PosCal\"&\" \"&N2","v":"PosCal EIN3"},null,{"$f":"\"PosCal\"&\" \"&P2","v":"PosCal ERF1"},null,{"$f":"\"PosCal\"&\" \"&R2","v":"PosCal WIPK"},null,{"$f":"\"PosCal\"&\" \"&T2","v":"PosCal JAR1"},null,{"$f":"\"PosCal\"&\" \"&V2","v":"PosCal coi1"},null,{"$f":"\"PosCal\"&\" \"&X2","v":"PosCal StPPS3"},null," - kalibracijski vzorec - pool, za primerjavo med ploš?icami"]
["P"]
[]
[null,"References",{"$nulls":5},"Targets"]
[null,"18S",null,"COX",null,"EF-2",null,"ETR1",null,"ETR4",null,"CTR1",null,"EIN3",null,"ERF1",null,"WIPK",null,"JAR1",null,"coi1",null,"StPPS3"]
[null,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24]
["A","kont24+1",null,"kont24+1",null,"kont24+1",null,"kont24+1",null,"kont24+1",null,"kont24+1",null,"kont24+1",null,"kont24+1",null,"kont24+1",null,"kont24+1",null,"kont24+1",null,"kont24+1"]
["B","kont24+2",null,"kont24+2",null,"kont24+2",null,"kont24+2",null,"kont24+2",null,"kont24+2",null,"kont24+2",null,"kont24+2",null,"kont24+2",null,"kont24+2",null,"kont24+2",null,"kont24+2"]
["C","kont24+3",null,"kont24+3",null,"kont24+3",null,"kont24+3",null,"kont24+3",null,"kont24+3",null,"kont24+3",null,"kont24+3",null,"kont24+3",null,"kont24+3",null,"kont24+3",null,"kont24+3"]
["D","bliz24+1",null,"bliz24+1",null,"bliz24+1",null,"bliz24+1",null,"bliz24+1",null,"bliz24+1",null,"bliz24+1",null,"bliz24+1",null,"bliz24+1",null,"bliz24+1",null,"bliz24+1",null,"bliz24+1"]
["E","bliz24+2",null,"bliz24+2",null,"bliz24+2",null,"bliz24+2",null,"bliz24+2",null,"bliz24+2",null,"bliz24+2",null,"bliz24+2",null,"bliz24+2",null,"bliz24+2",null,"bliz24+2",null,"bliz24+2"]
["F","bliz24+3",null,"bliz24+3",null,"bliz24+3",null,"bliz24+3",null,"bliz24+3",null,"bliz24+3",null,"bliz24+3",null,"bliz24+3",null,"bliz24+3",null,"bliz24+3",null,"bliz24+3",null,"bliz24+3"]
["G","inok24+1",null,"inok24+1",null,"inok24+1",null,"inok24+1",null,"inok24+1",null,"inok24+1",null,"inok24+1",null,"inok24+1",null,"inok24+1",null,"inok24+1",null,"inok24+1",null,"inok24+1"]
["H","inok24+2",null,"inok24+2",null,"inok24+2",null,"inok24+2",null,"inok24+2",null,"inok24+2",null,"inok24+2",null,"inok24+2",null,"inok24+2",null,"inok24+2",null,"inok24+2",null,"inok24+2"]
["I","inok24+3",null,"inok24+3",null,"inok24+3",null,"inok24+3",null,"inok24+3",null,"inok24+3",null,"inok24+3",null,"inok24+3",null,"inok24+3",null,"inok24+3",null,"inok24+3",null,"inok24+3"]
["J","ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc"]
["K"]
["L"]
["M"]
["N"]
["O",{"$f":"\"PosCal\"&\" \"&B22","v":"PosCal 18S"},null,{"$f":"\"PosCal\"&\" \"&D22","v":"PosCal COX"},null,{"$f":"\"PosCal\"&\" \"&F22","v":"PosCal EF-2"},null,{"$f":"\"PosCal\"&\" \"&H22","v":"PosCal ETR1"},null,{"$f":"\"PosCal\"&\" \"&J22","v":"PosCal ETR4"},null,{"$f":"\"PosCal\"&\" \"&L22","v":"PosCal CTR1"},null,{"$f":"\"PosCal\"&\" \"&N22","v":"PosCal EIN3"},null,{"$f":"\"PosCal\"&\" \"&P22","v":"PosCal ERF1"},null,{"$f":"\"PosCal\"&\" \"&R22","v":"PosCal WIPK"},null,{"$f":"\"PosCal\"&\" \"&T22","v":"PosCal JAR1"},null,{"$f":"\"PosCal\"&\" \"&V22","v":"PosCal coi1"},null,{"$f":"\"PosCal\"&\" \"&X22","v":"PosCal StPPS3"}]
["P"]
[null,"Slabosti te postavitve:"]
[null," - pripravljanje 6 mixov (60ul/ampl) na eno ploš?ico - SLABŠE ZA PIPETIRANJE Z ROBOTOM"]
[null," - na dveh ploš?icah analiziram samo 21 vzorcev in 3 tar?ne gene -> cel exp (84 vzorcev, 36 genov): 2*4*12 = 96 ploš?ic"]
[null," - ne moreš vedet ali gre pri vzorcu, ki odstopa za biol. variabilnost ali inhibicije!"]
[]
[null,"Prednosti te postavitve:"]
[null," - avtomati?na obdelava podatkov v Roche software-u"]
[null," - \"by the book\" postopek relativne kvantifikacije"]
[]
[null,"LEGENDA VZORCEV:"]
[null," - tretma: kont, bliz, inok",{"$nulls":5},3]
[null," - ?asovna to?ka: 0, 3h, 24h",{"$nulls":5},2]
[null," - skupina: +, - , wt, coi",{"$nulls":5},4]
[null," - bio. pon.: 1, 2, 3",{"$nulls":5},3]
[{"$nulls":6},"sum:",{"$f":"H53*H52*H51*H50 + H53*H52","v":84}]

{"sheet":"LC480+EFF","index":2,"n_rows":53,"n_cols":26,"rows_kept":53,"cut":false,"hidden":true,"merged":[]}
[null,"References",{"$nulls":11},"Targets"]
[null,"18S_1k",null,"18S_10k",null,"COX_1k",null,"COX_10k",null,"EF-2_10",null,"EF-2_100",null,"ETR1_10",null,"ETR1_100",null,"ETR4_10",null,"ETR4_100",null,"CTR1_10",null,"CTR1_100"]
[null,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24]
["A","0+1",null,"0+1",null,"0+1",null,"0+1",null,"0+1",null,"0+1",null,"0+1",null,"0+1",null,"0+1",null,"0+1",null,"0+1",null,"0+1"]
["B","0+2",null,"0+2",null,"0+2",null,"0+2",null,"0+2",null,"0+2",null,"0+2",null,"0+2",null,"0+2",null,"0+2",null,"0+2",null,"0+2"]
["C","0+3",null,"0+3",null,"0+3",null,"0+3",null,"0+3",null,"0+3",null,"0+3",null,"0+3",null,"0+3",null,"0+3",null,"0+3",null,"0+3"]
["D","kont3+1",null,"kont3+1",null,"kont3+1",null,"kont3+1",null,"kont3+1",null,"kont3+1",null,"kont3+1",null,"kont3+1",null,"kont3+1",null,"kont3+1",null,"kont3+1",null,"kont3+1"]
["E","kont3+2",null,"kont3+2",null,"kont3+2",null,"kont3+2",null,"kont3+2",null,"kont3+2",null,"kont3+2",null,"kont3+2",null,"kont3+2",null,"kont3+2",null,"kont3+2",null,"kont3+2"]
["F","kont3+3",null,"kont3+3",null,"kont3+3",null,"kont3+3",null,"kont3+3",null,"kont3+3",null,"kont3+3",null,"kont3+3",null,"kont3+3",null,"kont3+3",null,"kont3+3",null,"kont3+3"]
["G","bliz3+1",null,"bliz3+1",null,"bliz3+1",null,"bliz3+1",null,"bliz3+1",null,"bliz3+1",null,"bliz3+1",null,"bliz3+1",null,"bliz3+1",null,"bliz3+1",null,"bliz3+1",null,"bliz3+1"]
["H","bliz3+2",null,"bliz3+2",null,"bliz3+2",null,"bliz3+2",null,"bliz3+2",null,"bliz3+2",null,"bliz3+2",null,"bliz3+2",null,"bliz3+2",null,"bliz3+2",null,"bliz3+2",null,"bliz3+2"]
["I","bliz3+3",null,"bliz3+3",null,"bliz3+3",null,"bliz3+3",null,"bliz3+3",null,"bliz3+3",null,"bliz3+3",null,"bliz3+3",null,"bliz3+3",null,"bliz3+3",null,"bliz3+3",null,"bliz3+3"]
["J","inok3+1",null,"inok3+1",null,"inok3+1",null,"inok3+1",null,"inok3+1",null,"inok3+1",null,"inok3+1",null,"inok3+1",null,"inok3+1",null,"inok3+1",null,"inok3+1",null,"inok3+1"]
["K","inok3+2",null,"inok3+2",null,"inok3+2",null,"inok3+2",null,"inok3+2",null,"inok3+2",null,"inok3+2",null,"inok3+2",null,"inok3+2",null,"inok3+2",null,"inok3+2",null,"inok3+2"]
["L","inok3+3",null,"inok3+3",null,"inok3+3",null,"inok3+3",null,"inok3+3",null,"inok3+3",null,"inok3+3",null,"inok3+3",null,"inok3+3",null,"inok3+3",null,"inok3+3",null,"inok3+3"]
["M","ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc"]
["N"]
["O",{"$f":"\"PosCal\"&\" \"&B2","v":"PosCal 18S_1k"},null,{"$f":"\"PosCal\"&\" \"&D2","v":"PosCal 18S_10k"},null,{"$f":"\"PosCal\"&\" \"&F2","v":"PosCal COX_1k"},null,{"$f":"\"PosCal\"&\" \"&H2","v":"PosCal COX_10k"},null,{"$f":"\"PosCal\"&\" \"&J2","v":"PosCal EF-2_10"},null,{"$f":"\"PosCal\"&\" \"&L2","v":"PosCal EF-2_100"},null,{"$f":"\"PosCal\"&\" \"&N2","v":"PosCal ETR1_10"},null,{"$f":"\"PosCal\"&\" \"&P2","v":"PosCal ETR1_100"},null,{"$f":"\"PosCal\"&\" \"&R2","v":"PosCal ETR4_10"},null,{"$f":"\"PosCal\"&\" \"&T2","v":"PosCal ETR4_100"},null,{"$f":"\"PosCal\"&\" \"&V2","v":"PosCal CTR1_10"},null,{"$f":"\"PosCal\"&\" \"&X2","v":"PosCal CTR1_100"},null," - kalibracijski vzorec - pool, za primerjavo med ploš?icai"]
["P"]
[]
[null,"References",{"$nulls":11},"Targets"]
[null,"18S_1k",null,"18S_10k",null,"COX_1k",null,"COX_10k",null,"EF-2_10",null,"EF-2_100",null,"ETR1_10",null,"ETR1_100",null,"ETR4_10",null,"ETR4_100",null,"CTR1_10",null,"CTR1_100"]
[null,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24]
["A","kont24+1",null,"kont24+1",null,"kont24+1",null,"kont24+1",null,"kont24+1",null,"kont24+1",null,"kont24+1",null,"kont24+1",null,"kont24+1",null,"kont24+1",null,"kont24+1",null,"kont24+1"]
["B","kont24+2",null,"kont24+2",null,"kont24+2",null,"kont24+2",null,"kont24+2",null,"kont24+2",null,"kont24+2",null,"kont24+2",null,"kont24+2",null,"kont24+2",null,"kont24+2",null,"kont24+2"]
["C","kont24+3",null,"kont24+3",null,"kont24+3",null,"kont24+3",null,"kont24+3",null,"kont24+3",null,"kont24+3",null,"kont24+3",null,"kont24+3",null,"kont24+3",null,"kont24+3",null,"kont24+3"]
["D","bliz24+1",null,"bliz24+1",null,"bliz24+1",null,"bliz24+1",null,"bliz24+1",null,"bliz24+1",null,"bliz24+1",null,"bliz24+1",null,"bliz24+1",null,"bliz24+1",null,"bliz24+1",null,"bliz24+1"]
["E","bliz24+2",null,"bliz24+2",null,"bliz24+2",null,"bliz24+2",null,"bliz24+2",null,"bliz24+2",null,"bliz24+2",null,"bliz24+2",null,"bliz24+2",null,"bliz24+2",null,"bliz24+2",null,"bliz24+2"]
["F","bliz24+3",null,"bliz24+3",null,"bliz24+3",null,"bliz24+3",null,"bliz24+3",null,"bliz24+3",null,"bliz24+3",null,"bliz24+3",null,"bliz24+3",null,"bliz24+3",null,"bliz24+3",null,"bliz24+3"]
["G","inok24+1",null,"inok24+1",null,"inok24+1",null,"inok24+1",null,"inok24+1",null,"inok24+1",null,"inok24+1",null,"inok24+1",null,"inok24+1",null,"inok24+1",null,"inok24+1",null,"inok24+1"]
["H","inok24+2",null,"inok24+2",null,"inok24+2",null,"inok24+2",null,"inok24+2",null,"inok24+2",null,"inok24+2",null,"inok24+2",null,"inok24+2",null,"inok24+2",null,"inok24+2",null,"inok24+2"]
["I","inok24+3",null,"inok24+3",null,"inok24+3",null,"inok24+3",null,"inok24+3",null,"inok24+3",null,"inok24+3",null,"inok24+3",null,"inok24+3",null,"inok24+3",null,"inok24+3",null,"inok24+3"]
["J","ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc",null,"ntc"]
["K"]
["L"]
["M"]
["N"]
["O",{"$f":"\"PosCal\"&\" \"&B22","v":"PosCal 18S_1k"},null,{"$f":"\"PosCal\"&\" \"&D22","v":"PosCal 18S_10k"},null,{"$f":"\"PosCal\"&\" \"&F22","v":"PosCal COX_1k"},null,{"$f":"\"PosCal\"&\" \"&H22","v":"PosCal COX_10k"},null,{"$f":"\"PosCal\"&\" \"&J22","v":"PosCal EF-2_10"},null,{"$f":"\"PosCal\"&\" \"&L22","v":"PosCal EF-2_100"},null,{"$f":"\"PosCal\"&\" \"&N22","v":"PosCal ETR1_10"},null,{"$f":"\"PosCal\"&\" \"&P22","v":"PosCal ETR1_100"},null,{"$f":"\"PosCal\"&\" \"&R22","v":"PosCal ETR4_10"},null,{"$f":"\"PosCal\"&\" \"&T22","v":"PosCal ETR4_100"},null,{"$f":"\"PosCal\"&\" \"&V22","v":"PosCal CTR1_10"},null,{"$f":"\"PosCal\"&\" \"&X22","v":"PosCal CTR1_100"}]
["P"]
[null,"Slabosti te postavitve:"]
[null," - pripravljanje 12 mixov (60ul/ampl) na eno ploš?ico - SLABŠE ZA PIPETIRANJE Z ROBOTOM"]
[null," - na dveh ploš?icah analiziram samo 21 vzorcev in 9 tar?nih genov -> cel exp (84 vzorcev, 36 genov): 2*4*4 = 32 ploš?ic"]
[]
[null,"Prednosti te postavitve:"]
[null," - avtomati?na obdelava podatkov v Roche software-u"]
[null," - \"by the book\" postopek relativne kvantifikacije"]
[]
[null,"LEGENDA VZORCEV:"]
[null," - tretma: kont, bliz, inok",{"$nulls":5},3]
[null," - ?asovna to?ka: 0, 3h, 24h",{"$nulls":5},2]
[null," - skupina: +, - , wt, coi",{"$nulls":5},4]
[null," - bio. pon.: 1, 2, 3",{"$nulls":5},3]
[{"$nulls":6},"sum:",{"$f":"H52*H51*H50*H49 + H52*H51","v":84}]

{"sheet":"RNAinVAL_Ecoli_ds_production","index":3,"n_rows":44,"n_cols":36,"rows_kept":44,"cut":false,"hidden":false,"merged":[]}
[]
[{"$nulls":21},"pool",{"$nulls":8},"MasterMix used: ",null,null,null,"MasterMix used: "]
["amplikon",null,null,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,null,null,null,"Roche Probes Master with ROX",null,null,null,"SYBR Power Abi"]
["16S TaqMan",null,"A","vz1 (Ecoli_dsGFP) 100x",null,"vz1 1000x",null,"vz1 10 000x",null,"vz2 (Ecoli_dsMesh) 100x",null,"vz2 1000x",null,"vz2 10 000x",null,"vz3 (dsMesh K) 100x",null,"vz3 1000x",null,"vz3 10 000x",null,100,100,1000,1000,"10 000","10 000"]
[null,null,"B",{"$nulls":18},"100 000","100 000","1mio","1mio","NTC","NTC",null,null,null,"16S Taqman",null,null,null,"SYBR"]
["mesh_SYBR",null,"C","vz2 (Ecoli_dsMesh) 100x",null,"vz2 1000x",null,"vz2 10 000x",null,"vz3 (dsMesh K) 100x",null,"vz3 1000x",null,"vz3 10 000x",{"$nulls":7},100,100,1000,1000,"10 000","10 000",null,"vzorcev/amplikon:",null,3,null,null,null,2]
[null,null,"D",{"$nulls":18},"100 000","100 000","1mio","1mio","NTC","NTC",null,"lukenj/vz:",null,6,null,null,null,6]
["GFP_SYBR",null,"E","vz1 (Ecoli_dsGFP) 100x",null,"vz2 1000x",null,"vz2 10 000x",{"$nulls":13},100,100,1000,1000,"10 000","10 000",null,"NTC, umeritvena:",null,12,null,null,null,12]
[null,null,"F",{"$nulls":18},"100 000","100 000","1mio","1mio","NTC","NTC",null,"procent izgub:",null,10,"%",null,null,10,"%"]
[null,null,"G",{"$nulls":25},"št. reakcij z upoštevano izgubo:",null,{"$f":"(AE6*AE7+AE8)*(100+AE9)/100","v":33},null,null,null,{"$f":"(AI6*AI7+AI8)*(100+AI9)/100","v":26.4}]
[null,null,"H"]
[null,null,"I"]
[null,null,"J"]
[null,null,"K"]
[null,null,"L"]
[null,null,"M",{"$nulls":25},"TaqMan amplikoni",null,null,null,"SybrGreen amplikoni"]
[null,null,"N",{"$nulls":25},"št. reakcij:",{"$f":"ROUNDUP(AE10,0)","v":33},null,null,"št. reakcij:",{"$f":"ROUNDUP(AI10,0)","v":27}]
[null,null,"O",{"$nulls":25},"sonda:",150,7.4999999999999997E-2,null,"sonda:"]
[null,null,"P",{"$nulls":25},"FP:",300,0.05,null,"FP:",300,0.05]
[{"$nulls":28},"RP:",300,0.05,null,"RP:",300,0.05]
[]
[{"$nulls":4},"1)","za vzorce pripraviti redčine: 10x, 100x, 1000x, 10.000x"]
[{"$nulls":4},"2)","PRIPRAVITI POOL vz 1 in 2 (100x = 2ul + 198ul H2O)",{"$nulls":23},"MIX for each amplicon"]
[{"$nulls":4},"3)","pool redčit iz 100x, 1000x, 10 000x, 100 000x, 1 miox",{"$nulls":22},"UMM (2x)",{"$f":"2.5*AD17","v":82.5},"ul",null,"UMM (2x)",{"$f":"2.5*AH17","v":67.5},"ul"]
[{"$nulls":28},"FP",{"$f":"AE19*AD17","v":1.6500000000000001},"ul",null,"FP",{"$f":"AI19*AH17","v":1.35},"ul"]
[{"$nulls":28},"RP",{"$f":"AE19*AD17","v":1.6500000000000001},"ul",null,"RP",{"$f":"AI19*AH17","v":1.35},"ul"]
[{"$nulls":28},"sonda",{"$f":"AE18*AD17","v":2.4750000000000001},"ul",null,null,null,"ul"]
[{"$nulls":28},"ddH2O",{"$f":"(5*AD17)-(SUM(AD24:AD27,AD29))","v":10.724999999999994},"ul",null,"ddH2O",{"$f":"(5*AH17)-(SUM(AH24:AH27,AH29))","v":10.800000000000011},"ul"]
[{"$nulls":28},"vzorci",{"$f":"2*AD17","v":66},null,null,"vzorci",{"$f":"2*AH17","v":54}]
[{"$nulls":28},"skupaj mixa",{"$f":"SUM(AD24:AD28)","v":99},"ul",null,"skupaj mixa",{"$f":"SUM(AH24:AH28)","v":81},"ul"]
[{"$nulls":28},"pipetiramo",3,"ul",null,"pipetiramo",3,"ul"]
[{"$nulls":28},"skupaj Mix+Vz",{"$f":"+AD29+AD30","v":165},"ul",null,"skupaj Mix+Vz",{"$f":"+AH29+AH30","v":135},"ul"]
[{"$nulls":28},"ostane mixa",{"$f":"+AD30-(AE6*AE7+AE8)*3","v":9},"ul",null,"ostane mixa",{"$f":"+AH30-(AI6*AI7+AI8)*3","v":9},"ul"]
[]
[]
[{"$nulls":28},"primerji:"]
[{"$nulls":28},"Stock","Delovne raztopine za 5 ul reakcije:"]
[{"$nulls":28},"Primerji",null,"priprava","uM","ul v mix","V reakcije","konc/reakcijo","nM"]
[{"$nulls":28},"100 uM","30 uM","30+70",30,{"$f":"AI39/(AF39/AH39)","v":0.15},5,0.9,{"$f":"AF39*AG39/AH39*1000","v":900}]
[{"$nulls":31},30,{"$f":"AI40/(AF40/AH40)","v":9.9999999999999992E-2},5,0.6,{"$f":"AF40*AG40/AH40*1000","v":599.99999999999989}]
[{"$nulls":31},30,{"$f":"AI41/(AF41/AH41)","v":4.9999999999999996E-2},5,0.3,{"$f":"AF41*AG41/AH41*1000","v":299.99999999999994}]
[{"$nulls":28},"Sonda"]
[{"$nulls":28},"100 uM","10 uM","10+90",10,{"$f":"AI43/(AF43/AH43)","v":0.125},5,0.25,{"$f":"AF43*AG43/AH43*1000","v":250}]
[{"$nulls":31},10,{"$f":"AI44/(AF44/AH44)","v":7.4999999999999997E-2},5,0.15,{"$f":"AF44*AG44/AH44*1000","v":150}]